Gene description for NCKIPSD
Gene name NCK interacting protein with SH3 domain
Gene symbol NCKIPSD
Other names/aliases AF3P21
DIP
DIP1
ORF1
SPIN90
VIP54
WASLBP
WISH
Species Homo sapiens
 Database cross references - NCKIPSD
ExoCarta ExoCarta_51517
Vesiclepedia VP_51517
Entrez Gene 51517
HGNC 15486
MIM 606671
UniProt Q9NZQ3  
 NCKIPSD identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NCKIPSD
Molecular Function
    protein binding GO:0005515 IPI
    cytoskeletal protein binding GO:0008092 NAS
    SH3 domain binding GO:0017124 IEA
    Arp2/3 complex binding GO:0071933 IBA
Biological Process
    endocytosis GO:0006897 IBA
    cytoskeleton organization GO:0007010 NAS
    positive regulation of neuron projection development GO:0010976 IEA
    regulation of postsynapse assembly GO:0150052 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    intermediate filament GO:0005882 NAS
    COP9 signalosome GO:0008180 IDA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified NCKIPSD in exosomes
1
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NCKIPSD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 ACTR2 10097
Affinity Capture-MS Homo sapiens
3 ENKD1  
Two-hybrid Homo sapiens
4 HSPA6 3310
Co-fractionation Homo sapiens
5 SNX1 6642
Affinity Capture-MS Homo sapiens
6 SNX33 257364
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 RBM15B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 GIT2 9815
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 CRK 1398
Affinity Capture-MS Homo sapiens
10 PACSIN2 11252
Affinity Capture-MS Homo sapiens
11 HSPA1L 3305
Co-fractionation Homo sapiens
12 GEMIN8 54960
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
13 HSP90AB1 3326
Co-fractionation Homo sapiens
14 CYSLTR2  
Two-hybrid Homo sapiens
15 VEZF1  
Two-hybrid Homo sapiens
16 Actb 11461
Affinity Capture-MS Mus musculus
17 SMN2 6607
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
18 DIAPH3 81624
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 IQGAP1 8826
Affinity Capture-MS Homo sapiens
20 PSMD9 5715
Two-hybrid Homo sapiens
21 FBXO28  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 SNRPC 6631
Two-hybrid Homo sapiens
23 KHDRBS1 10657
Protein-peptide Homo sapiens
24 YWHAG 7532
Affinity Capture-MS Homo sapiens
25 LGALS4 3960
Two-hybrid Homo sapiens
26 PLCG1 5335
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 EPS15L1 58513
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 PALLD 23022
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 MGC50722  
Two-hybrid Homo sapiens
30 NCK1 4690
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 RXFP3  
Two-hybrid Homo sapiens
32 GEMIN2 8487
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 KIF14 9928
Affinity Capture-MS Homo sapiens
34 Tuba3a 22144
Affinity Capture-MS Mus musculus
35 DMRTB1  
Two-hybrid Homo sapiens
36 YWHAB 7529
Affinity Capture-MS Homo sapiens
37 ZC2HC1C  
Two-hybrid Homo sapiens
38 GEMIN5 25929
Affinity Capture-MS Homo sapiens
39 CPNE2 221184
Two-hybrid Homo sapiens
40 ATP5A1 498
Co-fractionation Homo sapiens
41 FASLG 356
Protein-peptide Homo sapiens
42 SMC6  
Affinity Capture-MS Homo sapiens
43 ZBTB47  
Two-hybrid Homo sapiens
44 ARMC8 25852
Affinity Capture-MS Homo sapiens
45 LRIF1  
Affinity Capture-MS Homo sapiens
46 ABI2 10152
Two-hybrid Homo sapiens
47 DDX20 11218
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
48 ACTR3B 57180
Affinity Capture-MS Homo sapiens
49 GIT1 28964
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 CSNK1A1 1452
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 MYH9 4627
Co-fractionation Homo sapiens
52 DCAF7 10238
Affinity Capture-MS Homo sapiens
53 ARHGEF6 9459
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 COPS3 8533
Affinity Capture-MS Homo sapiens
55 MED25  
Two-hybrid Homo sapiens
56 GEMIN7  
Affinity Capture-MS Homo sapiens
57 RAB11FIP5 26056
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 PRPF31 26121
Two-hybrid Homo sapiens
59 ACTG1 71
Affinity Capture-MS Homo sapiens
60 FGFR1OP  
Affinity Capture-MS Homo sapiens
61 YWHAE 7531
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 ANLN 54443
Affinity Capture-MS Homo sapiens
63 DST 667
Affinity Capture-MS Homo sapiens
64 IMMT 10989
Proximity Label-MS Homo sapiens
65 HSPA5 3309
Co-fractionation Homo sapiens
66 KIAA1462 57608
Proximity Label-MS Homo sapiens
67 CANX 821
Co-fractionation Homo sapiens
68 XPO1 7514
Affinity Capture-MS Homo sapiens
69 RC3H2  
Proximity Label-MS Homo sapiens
70 STAC  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
71 Nrip3  
Affinity Capture-MS Mus musculus
72 YWHAZ 7534
Affinity Capture-MS Homo sapiens
73 DACH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 CTTN 2017
Affinity Capture-MS Homo sapiens
75 USP54 159195
Proximity Label-MS Homo sapiens
76 PRPF3  
Co-fractionation Homo sapiens
77 RBP7  
Two-hybrid Homo sapiens
78 JUN 3725
Affinity Capture-MS Homo sapiens
79 GEMIN4 50628
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
80 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
81 TSEN54  
Two-hybrid Homo sapiens
82 SORBS3 10174
Two-hybrid Homo sapiens
83 ZFYVE9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 AKT1 207
Biochemical Activity Homo sapiens
85 GAS7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 BAIAP2 10458
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 GSN 2934
Affinity Capture-MS Homo sapiens
88 ARHGEF7 8874
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 ITSN1 6453
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 GRB2 2885
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 PDCD2L 84306
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 PIN1 5300
Two-hybrid Homo sapiens
93 Myo1c 17913
Affinity Capture-MS Mus musculus
94 CAPZA2 830
Affinity Capture-MS Homo sapiens
95 HSPB1 3315
Two-hybrid Homo sapiens
96 PSORS1C2  
Two-hybrid Homo sapiens
97 RBM15 64783
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 EPB41L2 2037
Co-fractionation Homo sapiens
99 GEMIN6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 NCK2 8440
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
101 Rassf1  
Affinity Capture-MS Mus musculus
102 ITSN2 50618
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 POLR3C 10623
Two-hybrid Homo sapiens
104 CIT 11113
Affinity Capture-MS Homo sapiens
105 USP9Y 8287
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 FCHSD1 89848
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here