Gene description for IFT74
Gene name intraflagellar transport 74
Gene symbol IFT74
Other names/aliases CCDC2
CMG-1
CMG1
Species Homo sapiens
 Database cross references - IFT74
ExoCarta ExoCarta_80173
Vesiclepedia VP_80173
Entrez Gene 80173
HGNC 21424
MIM 608040
UniProt Q96LB3  
 IFT74 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for IFT74
Molecular Function
    chromatin binding GO:0003682 IEA
    protein binding GO:0005515 IPI
    beta-tubulin binding GO:0048487 IBA
    beta-tubulin binding GO:0048487 IDA
Biological Process
    keratinocyte development GO:0003334 IEA
    Notch signaling pathway GO:0007219 IEA
    determination of left/right symmetry GO:0007368 IEA
    heart development GO:0007507 IEA
    negative regulation of keratinocyte proliferation GO:0010839 IEA
    positive regulation of cell adhesion mediated by integrin GO:0033630 IEA
    intraciliary anterograde transport GO:0035720 NAS
    intraciliary transport involved in cilium assembly GO:0035735 IBA
    intraciliary transport involved in cilium assembly GO:0035735 IMP
    keratinocyte proliferation GO:0043616 IEA
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    cilium assembly GO:0060271 IMP
    cilium assembly GO:0060271 NAS
    non-motile cilium assembly GO:1905515 IEA
Subcellular Localization
    acrosomal vesicle GO:0001669 IEA
    nucleus GO:0005634 IEA
    centrosome GO:0005813 IEA
    cilium GO:0005929 IBA
    cilium GO:0005929 NAS
    cilium GO:0005929 TAS
    intraciliary transport particle B GO:0030992 IBA
    intraciliary transport particle B GO:0030992 IPI
    intraciliary transport particle B GO:0030992 ISS
    motile cilium GO:0031514 ISS
    ciliary tip GO:0097542 TAS
 Experiment description of studies that identified IFT74 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IFT74
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCDC151  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 IFT27 11020
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 HOOK1  
Proximity Label-MS Homo sapiens
4 GNPDA2 132789
Affinity Capture-MS Homo sapiens
5 NPLOC4 55666
Affinity Capture-MS Homo sapiens
6 SYCE1  
Affinity Capture-MS Homo sapiens
7 UBXN10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
8 SQSTM1 8878
Proximity Label-MS Homo sapiens
9 TNRC18  
Cross-Linking-MS (XL-MS) Homo sapiens
10 C9orf78 51759
Affinity Capture-MS Homo sapiens
11 TTC30B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 APEX1 328
Affinity Capture-RNA Homo sapiens
13 SEPT9 10801
Proximity Label-MS Homo sapiens
14 IFT52 51098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 IFT88 8100
Affinity Capture-MS Homo sapiens
16 NIT1 4817
Affinity Capture-MS Homo sapiens
17 RHPN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 IFT81 28981
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 TULP3 7289
Affinity Capture-MS Homo sapiens
20 CEP135  
Proximity Label-MS Homo sapiens
21 KNSTRN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 AMOT  
Proximity Label-MS Homo sapiens
23 IFT20 90410
Affinity Capture-MS Homo sapiens
24 CCDC114  
Affinity Capture-MS Homo sapiens
25 NINL  
Proximity Label-MS Homo sapiens
26 C15orf59  
Affinity Capture-MS Homo sapiens
27 PFN1 5216
Proximity Label-MS Homo sapiens
28 KRT8 3856
Proximity Label-MS Homo sapiens
29 EGLN3  
Affinity Capture-MS Homo sapiens
30 MIB1 57534
Proximity Label-MS Homo sapiens
31 PRPH 5630
Proximity Label-MS Homo sapiens
32 HIST1H2BN 8341
Cross-Linking-MS (XL-MS) Homo sapiens
33 TPPP 11076
Co-fractionation Homo sapiens
34 LRRFIP1 9208
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 VCP 7415
Affinity Capture-MS Homo sapiens
36 DTNB 1838
Affinity Capture-MS Homo sapiens
37 FGF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 DCTN1 1639
Proximity Label-MS Homo sapiens
39 PEX14 5195
Proximity Label-MS Homo sapiens
40 IFT172 26160
Affinity Capture-MS Homo sapiens
41 RPA4  
Proximity Label-MS Homo sapiens
42 FEZF1  
Affinity Capture-MS Homo sapiens
43 HIST1H2BK 85236
Cross-Linking-MS (XL-MS) Homo sapiens
44 CT45A5  
Affinity Capture-MS Homo sapiens
45 TTC30A 92104
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 UFD1L 7353
Affinity Capture-MS Homo sapiens
47 TTC26 79989
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
48 CCDC28B  
Affinity Capture-MS Homo sapiens
49 ANKRD55  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 JPH4 84502
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 TRAF3IP1 26146
Affinity Capture-MS Homo sapiens
52 IFT57 55081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 GLI1  
Affinity Capture-MS Homo sapiens
54 NUP43 348995
Proximity Label-MS Homo sapiens
55 PTK2 5747
Proximity Label-MS Homo sapiens
56 MAPRE3  
Proximity Label-MS Homo sapiens
57 ABTB2 25841
Affinity Capture-MS Homo sapiens
58 IFT46 56912
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
59 NIN 51199
Proximity Label-MS Homo sapiens
60 TFPI2 7980
Affinity Capture-MS Homo sapiens
61 GOLGA1  
Proximity Label-MS Homo sapiens
62 NDC80 10403
Proximity Label-MS Homo sapiens
63 BICD1 636
Proximity Label-MS Homo sapiens
64 ZC3HAV1 56829
Affinity Capture-MS Homo sapiens
65 PCM1 5108
Proximity Label-MS Homo sapiens
66 ZC2HC1C  
Affinity Capture-MS Homo sapiens
67 KRT19 3880
Proximity Label-MS Homo sapiens
68 TSPYL4 23270
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 IFT22 64792
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 TXLNA 200081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 KRT18 3875
Proximity Label-MS Homo sapiens
72 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
73 ARL13B 200894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 HSPB11 51668
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 PLCD3 113026
Affinity Capture-MS Homo sapiens
76 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
77 SEPT10 151011
Proximity Label-MS Homo sapiens
78 DCTN2 10540
Proximity Label-MS Homo sapiens
79 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
80 SERPINB2 5055
Affinity Capture-MS Homo sapiens
81 CREB1  
Affinity Capture-MS Homo sapiens
82 IFT80 57560
Affinity Capture-MS Homo sapiens
83 ANAPC2 29882
Proximity Label-MS Homo sapiens
84 KDM1A 23028
Proximity Label-MS Homo sapiens
85 SIGLEC7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 PXN 5829
Proximity Label-MS Homo sapiens
87 PINK1  
Affinity Capture-MS Homo sapiens
88 KXD1 79036
Affinity Capture-MS Homo sapiens
89 ZUFSP 221302
Affinity Capture-MS Homo sapiens
90 EZR 7430
Proximity Label-MS Homo sapiens
91 C17orf59 54785
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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