Experiment description of studies that identified TOM1L1 in sEVs

1 Experiment ID 1203 MISEV standards ✔ EM Biophysical techniques ✔ SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 34887515     Organism Homo sapiens Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ." Journal name Nat Cell Biol Publication year 2021 Sample Colorectal cancer cells Sample name DiFi Isolation/purification methods Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation Flotation density - Molecules identified in the study Protein miRNA Methods used in the study Western blotting Mass spectrometry RNA sequencing

2 Experiment ID 363 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 364 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

4 Experiment ID 365 MISEV standards ✘ Biophysical techniques ✔ TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1 Enriched markers ✔ DCLK1 Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 33991177     Organism Homo sapiens Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M." Journal name Proteomics Publication year 2021 Sample Gastric cancer cells Sample name MKN1 - 100K pellet Isolation/purification methods Differential centrifugation Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

5 Experiment ID 211 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ cytochrome c|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name IGROV1 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

6 Experiment ID 212 MISEV standards ✔ CEM Biophysical techniques ✔ TSG101|Alix|EpCAM|TFRC Enriched markers ✔ Cytochrome C|GOLGA2 Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 23333927     Organism Homo sapiens Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes. Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K." Journal name J Proteomics Publication year 2013 Sample Ovarian cancer cells Sample name OVCAR-3 Isolation/purification methods Differential centrifugation Ultracentrifugation Sucrose density gradient Flotation density 1.09-1.15 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry

7 Experiment ID 275 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81 Enriched markers ✔ AIF Negative markers ✔ NTA Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 25844599     Organism Homo sapiens Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel. Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T." Journal name Oncotarget Publication year 2015 Sample Prostate cancer cells Sample name DU145 - Docetaxel sensitive Isolation/purification methods Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.12-1.19 g/mL Molecules identified in the study Protein Methods used in the study Mass spectrometry/Flow cytometry/Western blotting

8 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

9 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for TOM1L1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 P3H1 64175 Co-fractionation Homo sapiens 2 GSN 2934 Two-hybrid Homo sapiens 3 NIF3L1 60491 Co-fractionation Homo sapiens 4 TMEM174   Affinity Capture-MS Homo sapiens 5 CELF1 10658 Two-hybrid Homo sapiens 6 MRPS7 51081 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 7 GLUD2 2747 Two-hybrid Homo sapiens 8 ALAD 210 Co-fractionation Homo sapiens 9 CWF19L1 55280 Two-hybrid Homo sapiens 10 ZPR1 8882 Co-fractionation Homo sapiens 11 DPP9 91039 Co-fractionation Homo sapiens 12 ARPC5 10092 Co-fractionation Homo sapiens 13 TMED9 54732 Affinity Capture-MS Homo sapiens 14 COPS7A 50813 Two-hybrid Homo sapiens 15 UBXN4 23190 Two-hybrid Homo sapiens 16 STAT5B 6777 Co-fractionation Homo sapiens 17 PRKCD 5580 Co-fractionation Homo sapiens 18 TP53 7157 Synthetic Growth Defect Homo sapiens 19 ARPC1B 10095 Co-fractionation Homo sapiens 20 PDPN   Two-hybrid Homo sapiens 21 NRSN1   Affinity Capture-MS Homo sapiens 22 ACTR2 10097 Co-fractionation Homo sapiens 23 CAV3 859 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 24 AARS 16 Co-fractionation Homo sapiens 25 HSPBP1 23640 Co-fractionation Homo sapiens 26 ARPC4 10093 Co-fractionation Homo sapiens 27 TOLLIP 54472 Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens 28 PCDHGB4 8641 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 MANSC1 54682 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 30 NOMO1 23420 Two-hybrid Homo sapiens 31 TSG101 7251 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 32 ARPC2 10109 Co-fractionation Homo sapiens 33 UBC 7316 Reconstituted Complex Homo sapiens 34 EGFR 1956 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 35 OSGEP 55644 Co-fractionation Homo sapiens 36 ARHGEF16 27237 Co-fractionation Homo sapiens 37 AGPAT3 56894 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 38 AMFR 267 Two-hybrid Homo sapiens 39 PHYH 5264 Two-hybrid Homo sapiens 40 TMEM74   Affinity Capture-MS Homo sapiens 41 PWP2 5822 Two-hybrid Homo sapiens 42 GGA1 26088 Co-fractionation Homo sapiens 43 A2M 2 Co-fractionation Homo sapiens 44 NIPA1   Affinity Capture-MS Homo sapiens 45 CTXN1   Affinity Capture-MS Homo sapiens 46 SURF2   Co-fractionation Homo sapiens 47 KCNE3   Affinity Capture-MS Homo sapiens 48 SCRN2 90507 Co-fractionation Homo sapiens 49 TUBA1B 10376 Two-hybrid Homo sapiens 50 FYN 2534 Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 51 BAG1 573 Co-fractionation Homo sapiens 52 VCP 7415 Two-hybrid Homo sapiens 53 OGT 8473 Co-fractionation Homo sapiens 54 CRTAP 10491 Co-fractionation Homo sapiens 55 TMEM136   Affinity Capture-MS Homo sapiens 56 HSPA1A 3303 Affinity Capture-MS Homo sapiens 57 RPRD1B 58490 Co-fractionation Homo sapiens 58 NDC80 10403 Two-hybrid Homo sapiens 59 TP53RK 112858 Co-fractionation Homo sapiens 60 PKN1 5585 Co-fractionation Homo sapiens 61 HGS 9146 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 62 SRC 6714 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 63 DNAJB13   Two-hybrid Homo sapiens 64 CLTC 1213 Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens Affinity Capture-Western Homo sapiens Co-localization Homo sapiens Affinity Capture-MS Homo sapiens 65 VAV2 7410 Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 66 CUEDC1 404093 Affinity Capture-MS Homo sapiens 67 PELP1 27043 Two-hybrid Homo sapiens 68 MAP3K3 4215 Two-hybrid Homo sapiens 69 UBC 444874 Reconstituted Complex Bos taurus 70 MAPK12   Co-fractionation Homo sapiens 71 CAV1 857 Affinity Capture-MS Homo sapiens 72 SP100 6672 Co-fractionation Homo sapiens 73 GRB2 2885 Reconstituted Complex Homo sapiens 74 METTL1 4234 Co-fractionation Homo sapiens 75 PPME1 51400 Co-fractionation Homo sapiens 76 CCDC47 57003 Two-hybrid Homo sapiens 77 CTLA4   Affinity Capture-MS Homo sapiens View the network

Pathways in which TOM1L1 is involved

No pathways found