Gene description for DNAJC3
Gene name DnaJ (Hsp40) homolog, subfamily C, member 3
Gene symbol DNAJC3
Other names/aliases ACPHD
ERdj6
HP58
P58
P58IPK
PRKRI
Species Homo sapiens
 Database cross references - DNAJC3
ExoCarta ExoCarta_5611
Entrez Gene 5611
HGNC 9439
MIM 601184
UniProt Q13217  
 DNAJC3 identified in exosomes derived from the following tissue/cell type
Saliva 19199708    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for DNAJC3
Molecular Function
    protein kinase inhibitor activity GO:0004860 TAS
    chaperone binding GO:0051087 IEA
    misfolded protein binding GO:0051787 IEA
Biological Process
    defense response to virus GO:0051607 IEA
    cellular protein metabolic process GO:0044267 TAS
    IRE1-mediated unfolded protein response GO:0036498 TAS
    negative regulation of apoptotic process GO:0043066 IMP
    proteolysis involved in cellular protein catabolic process GO:0051603 IEA
    negative regulation of protein kinase activity GO:0006469 TAS
    negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation GO:1903912 IMP
    endoplasmic reticulum unfolded protein response GO:0030968 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    membrane GO:0016020 IDA
    smooth endoplasmic reticulum GO:0005790 IEA
    endoplasmic reticulum Sec complex GO:0031205 IEA
    cytoplasm GO:0005737 TAS
    extracellular vesicle GO:1903561 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
 Experiment description of studies that identified DNAJC3 in exosomes
1
Experiment ID 66
ISEV standards
IEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
CD63|CD81
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
2
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DNAJC3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF2AK3  
Affinity Capture-Western Homo sapiens
Phenotypic Suppression Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 PRKRIR  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 DNAJB1 3337
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 EIF2AK2 5610
Reconstituted Complex Homo sapiens
5 HSPA4 3308
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which DNAJC3 is involved
PathwayEvidenceSource
Viral mRNA Translation TAS Reactome
XBP1(S) activates chaperone genes TAS Reactome





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