Gene description for GCC2
Gene name GRIP and coiled-coil domain containing 2
Gene symbol GCC2
Other names/aliases GCC185
RANBP2L4
REN53
Species Homo sapiens
 Database cross references - GCC2
ExoCarta ExoCarta_9648
Vesiclepedia VP_9648
Entrez Gene 9648
HGNC 23218
MIM 612711
UniProt Q8IWJ2  
 GCC2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GCC2
Molecular Function
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 ISS
    identical protein binding GO:0042802 IPI
Biological Process
    protein targeting to lysosome GO:0006622 IMP
    microtubule organizing center organization GO:0031023 IMP
    protein localization to Golgi apparatus GO:0034067 IMP
    microtubule anchoring GO:0034453 IMP
    late endosome to Golgi transport GO:0034499 IBA
    late endosome to Golgi transport GO:0034499 IMP
    retrograde transport, endosome to Golgi GO:0042147 IMP
    Golgi to plasma membrane protein transport GO:0043001 IMP
    regulation of protein exit from endoplasmic reticulum GO:0070861 IMP
    recycling endosome to Golgi transport GO:0071955 IMP
    Golgi ribbon formation GO:0090161 IMP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    Golgi apparatus GO:0005794 IBA
    Golgi apparatus GO:0005794 IDA
    trans-Golgi network GO:0005802 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified GCC2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GCC2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 GJD3  
Proximity Label-MS Homo sapiens
3 AP2B1 163
Proximity Label-MS Homo sapiens
4 METTL7A 25840
Proximity Label-MS Homo sapiens
5 LAMP3  
Proximity Label-MS Homo sapiens
6 GJA1 2697
Proximity Label-MS Homo sapiens
7 YWHAE 7531
Affinity Capture-MS Homo sapiens
8 SPERT  
Affinity Capture-MS Homo sapiens
9 LAMP2 3920
Proximity Label-MS Homo sapiens
10 PMF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 B3GAT1  
Proximity Label-MS Homo sapiens
12 MLLT4 4301
Proximity Label-MS Homo sapiens
13 Cep55 74107
Affinity Capture-MS Mus musculus
14 Cetn2  
Affinity Capture-MS Mus musculus
15 CCDC53 51019
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 FBXW8 26259
Affinity Capture-MS Homo sapiens
17 YWHAG 7532
Affinity Capture-MS Homo sapiens
18 STX6 10228
Proximity Label-MS Homo sapiens
19 MLLT6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 HINT3 135114
Affinity Capture-MS Homo sapiens
21 Mcm3 17215
Affinity Capture-MS Mus musculus
22 ARL1 400
Two-hybrid Homo sapiens
23 BRK1 55845
Affinity Capture-MS Homo sapiens
24 EBAG9 9166
Proximity Label-MS Homo sapiens
25 EEF1A1 1915
Cross-Linking-MS (XL-MS) Homo sapiens
26 RAD23A 5886
Affinity Capture-MS Homo sapiens
27 EXOC3 11336
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CLASP2 23122
Affinity Capture-MS Homo sapiens
29 SMC4 10051
Cross-Linking-MS (XL-MS) Homo sapiens
30 YWHAB 7529
Affinity Capture-MS Homo sapiens
31 NUP155 9631
Proximity Label-MS Homo sapiens
32 MAPK1 5594
Affinity Capture-MS Homo sapiens
33 PFN1 5216
Proximity Label-MS Homo sapiens
34 SPC25 57405
Affinity Capture-MS Homo sapiens
35 TCP10L  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 TTC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 MRE11A 4361
Co-fractionation Homo sapiens
38 RPS10 6204
Cross-Linking-MS (XL-MS) Homo sapiens
39 MAPRE1 22919
Affinity Capture-MS Homo sapiens
40 RAB4A 5867
Proximity Label-MS Homo sapiens
41 TUBA1B 10376
Affinity Capture-MS Homo sapiens
42 PSMD2 5708
Affinity Capture-MS Homo sapiens
43 SYNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 CCDC107  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 PEX14 5195
Proximity Label-MS Homo sapiens
46 ELOVL5 60481
Proximity Label-MS Homo sapiens
47 WHAMMP3  
Affinity Capture-MS Homo sapiens
48 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
49 YWHAZ 7534
Affinity Capture-MS Homo sapiens
50 LMNB1 4001
Affinity Capture-MS Homo sapiens
51 PSMD13 5719
Affinity Capture-MS Homo sapiens
52 ATG12  
Proximity Label-MS Homo sapiens
53 HSD3B7 80270
Proximity Label-MS Homo sapiens
54 CUL3 8452
Affinity Capture-MS Homo sapiens
55 NR3C1 2908
Proximity Label-MS Homo sapiens
56 TUBB4B 10383
Affinity Capture-MS Homo sapiens
57 TFPT  
Affinity Capture-MS Homo sapiens
58 NAP1L1 4673
Cross-Linking-MS (XL-MS) Homo sapiens
59 RAB9A 9367
Proximity Label-MS Homo sapiens
60 GOLGA1  
Proximity Label-MS Homo sapiens
61 NHP2L1 4809
Co-fractionation Homo sapiens
62 NDC80 10403
Proximity Label-MS Homo sapiens
63 PSMC5 5705
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 PKD2 5311
Affinity Capture-MS Homo sapiens
65 PCM1 5108
Proximity Label-MS Homo sapiens
66 KRT19 3880
Proximity Label-MS Homo sapiens
67 PSMB1 5689
Affinity Capture-MS Homo sapiens
68 POLR1D 51082
Affinity Capture-MS Homo sapiens
69 AIMP1 9255
Affinity Capture-MS Homo sapiens
70 ZRANB1 54764
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 ANKFY1 51479
Proximity Label-MS Homo sapiens
72 SPOP  
Two-hybrid Homo sapiens
73 BCAS2 10286
Affinity Capture-MS Homo sapiens
74 LAMTOR1 55004
Proximity Label-MS Homo sapiens
75 PDIA4 9601
Co-fractionation Homo sapiens
76 ADCYAP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 LCK 3932
Proximity Label-MS Homo sapiens
78 SEPT10 151011
Proximity Label-MS Homo sapiens
79 DCTN2 10540
Proximity Label-MS Homo sapiens
80 KIFAP3 22920
Affinity Capture-MS Homo sapiens
81 GORASP1 64689
Proximity Label-MS Homo sapiens
82 ANAPC2 29882
Proximity Label-MS Homo sapiens
83 UFL1 23376
Affinity Capture-MS Homo sapiens
84 CLASP1 23332
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
85 KDM1A 23028
Proximity Label-MS Homo sapiens
86 DHX16 8449
Co-fractionation Homo sapiens
87 RAB2A 5862
Proximity Label-MS Homo sapiens
88 Dctn3  
Affinity Capture-MS Mus musculus
89 PXN 5829
Proximity Label-MS Homo sapiens
90 RAB11A 8766
Proximity Label-MS Homo sapiens
91 KXD1 79036
Affinity Capture-MS Homo sapiens
92 RAB5C 5878
Proximity Label-MS Homo sapiens
93 CCDC136  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 SSC5D 284297
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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