Gene description for DIAPH3
Gene name diaphanous-related formin 3
Gene symbol DIAPH3
Other names/aliases AN
AUNA1
DIA2
DRF3
NSDAN
diap3
mDia2
Species Homo sapiens
 Database cross references - DIAPH3
ExoCarta ExoCarta_81624
Vesiclepedia VP_81624
Entrez Gene 81624
HGNC 15480
MIM 614567
UniProt Q9NSV4  
 DIAPH3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for DIAPH3
Molecular Function
    actin binding GO:0003779 IEA
    microtubule binding GO:0008017 IEA
    small GTPase binding GO:0031267 IEA
    protein homodimerization activity GO:0042803 IEA
    cadherin binding GO:0045296 HDA
Biological Process
    in utero embryonic development GO:0001701 IEA
    cytoskeleton organization GO:0007010 ISS
    negative regulation of microtubule depolymerization GO:0007026 IEA
    chromosome segregation GO:0007059 IEA
    integrin-mediated signaling pathway GO:0007229 IEA
    sensory perception of sound GO:0007605 IEA
    gene expression GO:0010467 IEA
    endosomal transport GO:0016197 IEA
    establishment of cell polarity GO:0030010 IEA
    cell projection organization GO:0030030 IEA
    actin cytoskeleton organization GO:0030036 ISS
    actin filament polymerization GO:0030041 IBA
    actin filament polymerization GO:0030041 ISS
    macrophage differentiation GO:0030225 IEA
    protein-containing complex remodeling GO:0034367 IEA
    erythrocyte enucleation GO:0043131 IEA
    actin nucleation GO:0045010 IEA
    microtubule polymerization GO:0046785 IEA
    actin filament bundle assembly GO:0051017 IEA
    actin crosslink formation GO:0051764 IEA
    inner ear receptor cell differentiation GO:0060113 IEA
    head development GO:0060322 IEA
    autophagosome-lysosome fusion GO:0061909 IEA
    podosome assembly GO:0071800 IEA
Subcellular Localization
    ESCRT I complex GO:0000813 IEA
    spindle pole GO:0000922 IEA
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISS
    microtubule organizing center GO:0005815 IEA
    cytosol GO:0005829 TAS
    actin filament GO:0005884 IBA
    filamentous actin GO:0031941 IEA
    cleavage furrow GO:0032154 IEA
    actin filament bundle GO:0032432 IEA
    ribbon synapse GO:0097470 IEA
    stereocilia tip-link density GO:1990427 IEA
 Experiment description of studies that identified DIAPH3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for DIAPH3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PCM1 5108
Proximity Label-MS Homo sapiens
2 GYPA  
Affinity Capture-MS Homo sapiens
3 PHLPP1  
Proximity Label-MS Homo sapiens
4 CCDC96  
Affinity Capture-MS Homo sapiens
5 LAMTOR1 55004
Proximity Label-MS Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 FASN 2194
Positive Genetic Homo sapiens
8 DCTN2 10540
Proximity Label-MS Homo sapiens
9 RAB35 11021
Proximity Label-MS Homo sapiens
10 KIF14 9928
Affinity Capture-MS Homo sapiens
11 RPL37A 6168
Cross-Linking-MS (XL-MS) Homo sapiens
12 NXF1 10482
Affinity Capture-RNA Homo sapiens
13 CCNB2  
Co-fractionation Homo sapiens
14 BTNL9  
Affinity Capture-MS Homo sapiens
15 XPO1 7514
Affinity Capture-MS Homo sapiens
16 WWOX 51741
Affinity Capture-MS Homo sapiens
17 PFN1 5216
Proximity Label-MS Homo sapiens
18 TPTE2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 KRT8 3856
Proximity Label-MS Homo sapiens
20 TOLLIP 54472
Proximity Label-MS Homo sapiens
21 EZR 7430
Proximity Label-MS Homo sapiens
22 CCDC183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 HS2ST1 9653
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SQSTM1 8878
Proximity Label-MS Homo sapiens
25 C19orf38  
Affinity Capture-MS Homo sapiens
26 NCKIPSD 51517
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
27 TMEM126A 84233
Affinity Capture-MS Homo sapiens
28 VTA1 51534
Affinity Capture-MS Homo sapiens
29 SIRT1  
Affinity Capture-MS Homo sapiens
30 PNKD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 MAPRE1 22919
Proximity Label-MS Homo sapiens
32 CENPA  
Affinity Capture-Western Homo sapiens
33 CDC42 998
Reconstituted Complex Homo sapiens
34 ANAPC2 29882
Proximity Label-MS Homo sapiens
35 NDC80 10403
Proximity Label-MS Homo sapiens
36 RAB5A 5868
Proximity Label-MS Homo sapiens
37 MAPRE3  
Proximity Label-MS Homo sapiens
38 PRKACA 5566
Proximity Label-MS Homo sapiens
39 COMTD1 118881
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 TRIM37  
Proximity Label-MS Homo sapiens
41 CCNB1 891
Co-fractionation Homo sapiens
42 S100A4 6275
Affinity Capture-MS Homo sapiens
43 CDH1 999
Proximity Label-MS Homo sapiens
44 C3orf18  
Affinity Capture-MS Homo sapiens
45 ENPP6 133121
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 SMAD9  
Two-hybrid Homo sapiens
47 VCP 7415
Affinity Capture-MS Homo sapiens
48 RAC1 5879
Reconstituted Complex Homo sapiens
49 RAB11A 8766
Proximity Label-MS Homo sapiens
50 GOLGA1  
Proximity Label-MS Homo sapiens
51 BCHE 590
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 SIX5  
Affinity Capture-MS Homo sapiens
53 DCTN1 1639
Proximity Label-MS Homo sapiens
54 ACTB 60
Proximity Label-MS Homo sapiens
55 SCN2B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 SNW1 22938
Affinity Capture-MS Homo sapiens
57 ENO1 2023
Affinity Capture-RNA Homo sapiens
58 EBAG9 9166
Proximity Label-MS Homo sapiens
59 RHOB 388
Proximity Label-MS Homo sapiens
60 KRAS 3845
Proximity Label-MS Homo sapiens
61 HDAC6 10013
Affinity Capture-Western Homo sapiens
62 VASN 114990
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 RHOA 387
Reconstituted Complex Homo sapiens
64 RPA3 6119
Proximity Label-MS Homo sapiens
65 CAV1 857
Proximity Label-MS Homo sapiens
66 EPHA1 2041
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 STX6 10228
Proximity Label-MS Homo sapiens
68 ACBD5 91452
Proximity Label-MS Homo sapiens
69 RMDN3 55177
Proximity Label-MS Homo sapiens
70 CAPN2 824
Affinity Capture-MS Homo sapiens
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