Gene description for CENPE
Gene name centromere protein E, 312kDa
Gene symbol CENPE
Other names/aliases CENP-E
KIF10
MCPH13
PPP1R61
Species Homo sapiens
 Database cross references - CENPE
ExoCarta ExoCarta_1062
Entrez Gene 1062
HGNC 1856
MIM 117143
UniProt Q02224  
 CENPE identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
Prostate cancer cells 25844599    
 Gene ontology annotations for CENPE
Molecular Function
    microtubule motor activity GO:0003777 IMP
    ATPase activity GO:0016887 IBA
    ATP binding GO:0005524 IEA
    protein binding GO:0005515 IPI
    kinetochore binding GO:0043515 IDA
    microtubule binding GO:0008017 IEA
Biological Process
    metabolic process GO:0008152 IMP
    mitotic chromosome movement towards spindle pole GO:0007079 TAS
    multicellular organismal development GO:0007275 IEA
    blood coagulation GO:0007596 TAS
    mitotic cell cycle GO:0000278 TAS
    positive regulation of protein kinase activity GO:0045860 IMP
    attachment of mitotic spindle microtubules to kinetochore GO:0051315 IMP
    chromosome segregation GO:0007059 IMP
    microtubule-based movement GO:0007018 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    kinetochore assembly GO:0051382 NAS
    antigen processing and presentation of exogenous peptide antigen via MHC class II GO:0019886 TAS
    mitotic metaphase plate congression GO:0007080 IMP
    cell division GO:0051301 IEA
    regulation of mitotic metaphase/anaphase transition GO:0030071 IMP
Subcellular Localization
    midbody GO:0030496 IDA
    chromosome GO:0005694 IDA
    kinesin complex GO:0005871 IBA
    microtubule cytoskeleton GO:0015630 IDA
    condensed chromosome, centromeric region GO:0000779 IDA
    chromosome, centromeric region GO:0000775 IDA
    condensed nuclear chromosome kinetochore GO:0000778 IEA
    mitotic spindle midzone GO:1990023 IDA
    microtubule GO:0005874 IDA
    condensed chromosome outer kinetochore GO:0000940 TAS
    membrane GO:0016020 IDA
    nucleus GO:0005634 IMP
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IDA
    kinetochore GO:0000776 IDA
 Experiment description of studies that identified CENPE in exosomes
1
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
2
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for CENPE
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 BUB1B  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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