Gene description for PYCRL
Gene name pyrroline-5-carboxylate reductase-like
Gene symbol PYCRL
Other names/aliases -
Species Homo sapiens
 Database cross references - PYCRL
ExoCarta ExoCarta_65263
Vesiclepedia VP_65263
Entrez Gene 65263
HGNC 25846
UniProt Q53H96  
 PYCRL identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for PYCRL
Molecular Function
    pyrroline-5-carboxylate reductase activity GO:0004735 IBA
    pyrroline-5-carboxylate reductase activity GO:0004735 IDA
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IEA
Biological Process
    L-proline biosynthetic process GO:0055129 IBA
    L-proline biosynthetic process GO:0055129 IDA
    L-proline biosynthetic process GO:0055129 IEA
Subcellular Localization
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    intercellular bridge GO:0045171 IDA
    mitotic spindle GO:0072686 IDA
 Experiment description of studies that identified PYCRL in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PYCRL
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 PSMA6 5687
Co-fractionation Homo sapiens
3 PSMB2 5690
Co-fractionation Homo sapiens
4 HVCN1 84329
Affinity Capture-MS Homo sapiens
5 C16orf72 29035
Affinity Capture-MS Homo sapiens
6 GTF2F1 2962
Affinity Capture-MS Homo sapiens
7 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
8 MYCN  
Affinity Capture-MS Homo sapiens
9 CCDC6 8030
Affinity Capture-MS Homo sapiens
10 SDCBP 6386
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 KCNG1 3755
Affinity Capture-MS Homo sapiens
12 DNAJB4 11080
Affinity Capture-MS Homo sapiens
13 MAP7 9053
Affinity Capture-MS Homo sapiens
14 MTMR14 64419
Affinity Capture-MS Homo sapiens
15 UIMC1  
Affinity Capture-MS Homo sapiens
16 MICU2 221154
Affinity Capture-MS Homo sapiens
17 RPL23 9349
Affinity Capture-MS Homo sapiens
18 RIN3  
Affinity Capture-MS Homo sapiens
19 PSMA4 5685
Co-fractionation Homo sapiens
20 PCMT1 5110
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 C1orf106  
Affinity Capture-MS Homo sapiens
22 USP9X 8239
Affinity Capture-MS Homo sapiens
23 PDK1 5163
Affinity Capture-MS Homo sapiens
24 CYTH2 9266
Affinity Capture-MS Homo sapiens
25 UFL1 23376
Affinity Capture-MS Homo sapiens
26 FAM96A  
Affinity Capture-MS Homo sapiens
27 DGCR8  
Affinity Capture-MS Homo sapiens
28 ISCA1  
Affinity Capture-MS Homo sapiens
29 NXPH1  
Affinity Capture-MS Homo sapiens
30 CUL3 8452
Affinity Capture-MS Homo sapiens
31 TRIM33 51592
Affinity Capture-MS Homo sapiens
32 ZCCHC9  
Affinity Capture-MS Homo sapiens
33 WDYHV1  
Two-hybrid Homo sapiens
34 PYCR1 5831
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 GPD1L 23171
Co-fractionation Homo sapiens
36 FKBP6  
Affinity Capture-MS Homo sapiens
37 FUS 2521
Affinity Capture-MS Homo sapiens
38 PYCR2 29920
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 KLRD1  
Affinity Capture-MS Homo sapiens
40 AARSD1 80755
Affinity Capture-MS Homo sapiens
41 HLA-DPB1 3115
Affinity Capture-MS Homo sapiens
42 ABT1 29777
Affinity Capture-MS Homo sapiens
43 BABAM1 29086
Affinity Capture-MS Homo sapiens
44 PRMT6  
Two-hybrid Homo sapiens
45 FBXW7  
Affinity Capture-MS Homo sapiens
46 LACRT 90070
Affinity Capture-MS Homo sapiens
47 CDKN2B 1030
Two-hybrid Homo sapiens
48 SUPT6H 6830
Affinity Capture-MS Homo sapiens
49 DYRK1A 1859
Affinity Capture-MS Homo sapiens
50 PSMA7 5688
Co-fractionation Homo sapiens
51 ZMYND8 23613
Affinity Capture-MS Homo sapiens
52 ALDH3B2  
Affinity Capture-MS Homo sapiens
53 SNX11 29916
Affinity Capture-MS Homo sapiens
54 SPRTN  
Affinity Capture-MS Homo sapiens
55 Trp53bp1  
Affinity Capture-MS Mus musculus
56 KLHDC10 23008
Affinity Capture-MS Homo sapiens
57 KLHL26  
Affinity Capture-MS Homo sapiens
58 ZNF687  
Affinity Capture-MS Homo sapiens
59 PYCRL 65263
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
60 SERBP1 26135
Affinity Capture-MS Homo sapiens
61 PSMB5 5693
Co-fractionation Homo sapiens
62 MRPS18C  
Affinity Capture-MS Homo sapiens
63 PUSL1  
Affinity Capture-MS Homo sapiens
64 KCTD12 115207
Affinity Capture-MS Homo sapiens
65 CYTH1 9267
Affinity Capture-MS Homo sapiens
66 GPX7 2882
Affinity Capture-MS Homo sapiens
67 AKT1 207
Biochemical Activity Homo sapiens
68 DNAJB1 3337
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 PRMT1 3276
Affinity Capture-MS Homo sapiens
70 PSMB1 5689
Co-fractionation Homo sapiens
71 PDK2 5164
Affinity Capture-MS Homo sapiens
72 LBP 3929
Two-hybrid Homo sapiens
73 BAG4  
Two-hybrid Homo sapiens
74 ARFGAP1 55738
Affinity Capture-MS Homo sapiens
75 PSMB3 5691
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which PYCRL is involved
PathwayEvidenceSource
Glutamate and glutamine metabolism TAS Reactome
Metabolism TAS Reactome
Metabolism of amino acids and derivatives TAS Reactome





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