Gene description for WASH1
Gene name WAS protein family homolog 1
Gene symbol WASH1
Other names/aliases FAM39E
WASH
Species Homo sapiens
 Database cross references - WASH1
ExoCarta ExoCarta_100287171
Vesiclepedia VP_100287171
Entrez Gene 100287171
HGNC 24361
MIM 613632
UniProt A8K0Z3  
 WASH1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for WASH1
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    alpha-tubulin binding GO:0043014 IBA
    alpha-tubulin binding GO:0043014 IDA
    gamma-tubulin binding GO:0043015 IBA
    phosphatidylinositol 3-kinase inhibitor activity GO:0141039 ISS
Biological Process
    exocytosis GO:0006887 IBA
    exocytosis GO:0006887 IMP
    negative regulation of autophagy GO:0010507 ISS
    protein transport GO:0015031 IEA
    endosomal transport GO:0016197 ISS
    endosomal transport GO:0016197 NAS
    extracellular matrix disassembly GO:0022617 IMP
    positive regulation of cell migration GO:0030335 IMP
    positive regulation of pseudopodium assembly GO:0031274 IMP
    regulation of protein ubiquitination GO:0031396 ISS
    endocytic recycling GO:0032456 IBA
    endocytic recycling GO:0032456 IMP
    Arp2/3 complex-mediated actin nucleation GO:0034314 IBA
    Arp2/3 complex-mediated actin nucleation GO:0034314 IDA
    Arp2/3 complex-mediated actin nucleation GO:0034314 ISS
    regulation of Arp2/3 complex-mediated actin nucleation GO:0034315 NAS
    retrograde transport, endosome to Golgi GO:0042147 IBA
    retrograde transport, endosome to Golgi GO:0042147 IDA
    retrograde transport, endosome to Golgi GO:0042147 IMP
Subcellular Localization
    exocyst GO:0000145 IDA
    early endosome GO:0005769 IBA
    early endosome GO:0005769 IDA
    early endosome GO:0005769 ISS
    late endosome GO:0005770 IEA
    autophagosome GO:0005776 ISS
    centriole GO:0005814 IEA
    cytosol GO:0005829 IEA
    early endosome membrane GO:0031901 NAS
    recycling endosome GO:0055037 IBA
    recycling endosome GO:0055037 ISS
    recycling endosome membrane GO:0055038 IEA
    WASH complex GO:0071203 IBA
    WASH complex GO:0071203 IDA
    WASH complex GO:0071203 NAS
 Experiment description of studies that identified WASH1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for WASH1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBL5 59286
Two-hybrid Homo sapiens
2 KRT76 51350
Two-hybrid Homo sapiens
3 VPS35 55737
Affinity Capture-MS Homo sapiens
4 LIMK1 3984
Two-hybrid Homo sapiens
5 EIF3F 8665
Two-hybrid Homo sapiens
6 SNX27 81609
Affinity Capture-MS Homo sapiens
7 ABI3 51225
Two-hybrid Homo sapiens
8 LMO1  
Two-hybrid Homo sapiens
9 SDCBP 6386
Two-hybrid Homo sapiens
10 POTEKP 440915
Affinity Capture-MS Homo sapiens
11 HMGB2 3148
Affinity Capture-MS Homo sapiens
12 LMO4  
Two-hybrid Homo sapiens
13 BLOC1S2 282991
Two-hybrid Homo sapiens
14 KIAA0196 9897
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
15 EXOC8 149371
Two-hybrid Homo sapiens
16 BAG1 573
Affinity Capture-MS Homo sapiens
17 C1orf216  
Two-hybrid Homo sapiens
18 ACTA1 58
Affinity Capture-MS Homo sapiens
19 EEA1 8411
Co-localization Homo sapiens
20 RFWD2  
Affinity Capture-MS Homo sapiens
21 C14orf119  
Two-hybrid Homo sapiens
22 RABGEF1 27342
Two-hybrid Homo sapiens
23 KLC3  
Two-hybrid Homo sapiens
24 KLHDC3  
Affinity Capture-MS Homo sapiens
25 PRKAA2 5563
Two-hybrid Homo sapiens
26 SRP9 6726
Affinity Capture-MS Homo sapiens
27 NUP62CL  
Two-hybrid Homo sapiens
28 MRFAP1  
Two-hybrid Homo sapiens
29 IFT57 55081
Two-hybrid Homo sapiens
30 ACTBL2 345651
Affinity Capture-MS Homo sapiens
31 HSBP1 3281
Two-hybrid Homo sapiens
32 CEP55 55165
Two-hybrid Homo sapiens
33 FAM21A 387680
Co-fractionation Homo sapiens
34 EGFR 1956
Negative Genetic Homo sapiens
35 KRT28 162605
Two-hybrid Homo sapiens
36 CAPZB 832
Affinity Capture-MS Homo sapiens
37 ZBTB16  
Two-hybrid Homo sapiens
38 HSPA1A 3303
Affinity Capture-MS Homo sapiens
39 CDR2L  
Two-hybrid Homo sapiens
40 FHL2 2274
Two-hybrid Homo sapiens
41 CCDC93 54520
Affinity Capture-MS Homo sapiens
42 TRIM69 140691
Two-hybrid Homo sapiens
43 VPS29 51699
Affinity Capture-MS Homo sapiens
44 SRP14 6727
Affinity Capture-MS Homo sapiens
45 LMO2  
Two-hybrid Homo sapiens
46 TPM1 7168
Two-hybrid Homo sapiens
47 ABI2 10152
Two-hybrid Homo sapiens
48 WASH6P  
Affinity Capture-MS Homo sapiens
49 MORN4  
Two-hybrid Homo sapiens
50 BECN1 8678
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
51 WASH3P  
Affinity Capture-MS Homo sapiens
52 POLR1C 9533
Two-hybrid Homo sapiens
53 KRT3 3850
Two-hybrid Homo sapiens
54 RAB7A 7879
Proximity Label-MS Homo sapiens
55 GYS1 2997
Two-hybrid Homo sapiens
56 KRT75 9119
Two-hybrid Homo sapiens
57 KANK2 25959
Two-hybrid Homo sapiens
58 OSTF1 26578
Two-hybrid Homo sapiens
59 FAM21C 253725
Co-fractionation Homo sapiens
60 ATPAF2 91647
Two-hybrid Homo sapiens
61 HGS 9146
Co-localization Homo sapiens
Two-hybrid Homo sapiens
62 ACTB 60
Affinity Capture-MS Homo sapiens
63 KRT20 54474
Two-hybrid Homo sapiens
64 RNF2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
65 LOC100288778  
Affinity Capture-MS Homo sapiens
66 CCDC53 51019
Two-hybrid Homo sapiens
67 KRAS 3845
Negative Genetic Homo sapiens
68 OIP5  
Two-hybrid Homo sapiens
69 HSF2BP  
Two-hybrid Homo sapiens
70 ACTA2 59
Affinity Capture-MS Homo sapiens
71 GSPT1 2935
Affinity Capture-MS Homo sapiens
72 KIAA1033 23325
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
73 ACTG1 71
Affinity Capture-MS Homo sapiens
74 USP20  
Two-hybrid Homo sapiens
75 KRT26 353288
Two-hybrid Homo sapiens
76 TPM3 7170
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which WASH1 is involved
No pathways found





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