Gene description for PUS7
Gene name pseudouridylate synthase 7 (putative)
Gene symbol PUS7
Other names/aliases -
Species Homo sapiens
 Database cross references - PUS7
ExoCarta ExoCarta_54517
Vesiclepedia VP_54517
Entrez Gene 54517
HGNC 26033
MIM 616261
UniProt Q96PZ0  
 PUS7 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PUS7
Molecular Function
    RNA binding GO:0003723 HDA
    pseudouridine synthase activity GO:0009982 IBA
    pseudouridine synthase activity GO:0009982 IDA
    pseudouridine synthase activity GO:0009982 IMP
    enzyme binding GO:0019899 IPI
    tRNA pseudouridine(13) synthase activity GO:0160150 IDA
Biological Process
    pseudouridine synthesis GO:0001522 IBA
    mRNA processing GO:0006397 IEA
    RNA splicing GO:0008380 IEA
    negative regulation of translation GO:0017148 IDA
    negative regulation of translation GO:0017148 IMP
    tRNA pseudouridine synthesis GO:0031119 IDA
    regulation of hematopoietic stem cell differentiation GO:1902036 IMP
    mRNA pseudouridine synthesis GO:1990481 IDA
    mRNA pseudouridine synthesis GO:1990481 IMP
    regulation of mesoderm development GO:2000380 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified PUS7 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
EM
Biophysical techniques
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PUS7
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF4G3 8672
Co-fractionation Homo sapiens
2 POLR2G 5436
Co-fractionation Homo sapiens
3 MCM3 4172
Co-fractionation Homo sapiens
4 ZNF174  
Affinity Capture-MS Homo sapiens
5 SPRTN  
Affinity Capture-MS Homo sapiens
6 ACRBP  
Affinity Capture-MS Homo sapiens
7 MED17  
Affinity Capture-MS Homo sapiens
8 DNAJC8 22826
Affinity Capture-MS Homo sapiens
9 S100A6 6277
Affinity Capture-MS Homo sapiens
10 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
11 ACTC1 70
Proximity Label-MS Homo sapiens
12 STRN3 29966
Affinity Capture-MS Homo sapiens
13 BYSL 705
Co-fractionation Homo sapiens
14 SSB 6741
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
15 PPP4R2  
Affinity Capture-MS Homo sapiens
16 MILR1  
Affinity Capture-MS Homo sapiens
17 PEX1 5189
Affinity Capture-MS Homo sapiens
18 APEX1 328
Affinity Capture-RNA Homo sapiens
19 REG3A  
Affinity Capture-MS Homo sapiens
20 ERF  
Affinity Capture-MS Homo sapiens
21 DPYSL2 1808
Co-fractionation Homo sapiens
22 WDR74 54663
Co-fractionation Homo sapiens
23 ANKRD49  
Affinity Capture-MS Homo sapiens
24 STAT3 6774
Co-fractionation Homo sapiens
25 HSPH1 10808
Co-fractionation Homo sapiens
26 FBL 2091
Co-fractionation Homo sapiens
27 DNAJB1 3337
Proximity Label-MS Homo sapiens
28 ATP2A3 489
Affinity Capture-MS Homo sapiens
29 CES2 8824
Affinity Capture-MS Homo sapiens
30 CTPS1 1503
Co-fractionation Homo sapiens
31 ANAPC16  
Affinity Capture-MS Homo sapiens
32 RBM8A 9939
Co-fractionation Homo sapiens
33 GAR1 54433
Co-fractionation Homo sapiens
34 ENOPH1 58478
Co-fractionation Homo sapiens
35 UNC45A 55898
Affinity Capture-MS Homo sapiens
36 PTGES2 80142
Affinity Capture-MS Homo sapiens
37 NSUN2 54888
Co-fractionation Homo sapiens
38 IDE 3416
Co-fractionation Homo sapiens
39 ZNF444  
Affinity Capture-MS Homo sapiens
40 ACOT13  
Co-fractionation Homo sapiens
41 POLR3K  
Affinity Capture-MS Homo sapiens
42 PARK2  
Affinity Capture-MS Homo sapiens
43 APOBEC3C 27350
Affinity Capture-MS Homo sapiens
44 CBX3 11335
Proximity Label-MS Homo sapiens
45 ACTR3B 57180
Affinity Capture-MS Homo sapiens
46 BBS1 582
Affinity Capture-MS Homo sapiens
47 ACTN4 81
Co-fractionation Homo sapiens
48 NTRK1 4914
Affinity Capture-MS Homo sapiens
49 KDM4B  
Affinity Capture-MS Homo sapiens
50 LUC7L2 51631
Affinity Capture-MS Homo sapiens
51 S100B 6285
Affinity Capture-MS Homo sapiens
52 ENO1 2023
Co-fractionation Homo sapiens
53 HAUS7  
Affinity Capture-MS Homo sapiens
54 RYBP  
Affinity Capture-MS Homo sapiens
55 PIPSL 266971
Affinity Capture-MS Homo sapiens
56 ENO3 2027
Co-fractionation Homo sapiens
57 ZBTB2 57621
Affinity Capture-MS Homo sapiens
58 S100P 6286
Affinity Capture-MS Homo sapiens
59 CCM2 83605
Affinity Capture-MS Homo sapiens
60 RABGGTA 5875
Affinity Capture-MS Homo sapiens
61 THUMPD1 55623
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 NOC3L 64318
Co-fractionation Homo sapiens
63 ATP5F1 515
Affinity Capture-MS Homo sapiens
64 EIF4G1 1981
Co-fractionation Homo sapiens
65 DOK4 55715
Affinity Capture-MS Homo sapiens
66 SF1 7536
Two-hybrid Homo sapiens
67 SYT6  
Affinity Capture-MS Homo sapiens
68 TYW3  
Affinity Capture-MS Homo sapiens
69 DOHH 83475
Co-fractionation Homo sapiens
70 POLR2D  
Co-fractionation Homo sapiens
71 GBF1 8729
Co-fractionation Homo sapiens
72 ACTR6  
Co-fractionation Homo sapiens
73 DMAP1 55929
Affinity Capture-MS Homo sapiens
74 ARHGAP44  
Affinity Capture-MS Homo sapiens
75 TJP2 9414
Co-fractionation Homo sapiens
76 KIF1BP 26128
Affinity Capture-MS Homo sapiens
77 RPA3 6119
Proximity Label-MS Homo sapiens
78 AHR 196
Affinity Capture-MS Homo sapiens
79 SUV39H2 79723
Affinity Capture-MS Homo sapiens
80 NHP2L1 4809
Co-fractionation Homo sapiens
81 JUNB  
Affinity Capture-MS Homo sapiens
82 ENO2 2026
Co-fractionation Homo sapiens
83 NMD3 51068
Co-fractionation Homo sapiens
84 CLPB 81570
Affinity Capture-MS Homo sapiens
85 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
86 LMNA 4000
Proximity Label-MS Homo sapiens
87 CBWD1 55871
Affinity Capture-MS Homo sapiens
88 GATAD2A 54815
Affinity Capture-MS Homo sapiens
89 FTL 2512
Affinity Capture-MS Homo sapiens
90 DHX15 1665
Co-fractionation Homo sapiens
91 TMEM51 55092
Affinity Capture-MS Homo sapiens
92 PRKCSH 5589
Affinity Capture-MS Homo sapiens
93 RAD17  
Affinity Capture-MS Homo sapiens
94 TBC1D9  
Affinity Capture-MS Homo sapiens
95 DDX3X 1654
Co-fractionation Homo sapiens
96 IQGAP3 128239
Affinity Capture-MS Homo sapiens
97 CXXC4 80319
Affinity Capture-MS Homo sapiens
98 FGB 2244
Affinity Capture-MS Homo sapiens
99 SMAD4  
Affinity Capture-MS Homo sapiens
100 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
101 AGPAT3 56894
Affinity Capture-MS Homo sapiens
102 SIRT1  
Affinity Capture-MS Homo sapiens
103 PHKA2 5256
Affinity Capture-MS Homo sapiens
104 MCM5 4174
Co-fractionation Homo sapiens
105 DHX16 8449
Co-fractionation Homo sapiens
106 ERRFI1 54206
Affinity Capture-MS Homo sapiens
107 MND1  
Affinity Capture-MS Homo sapiens
108 RHOD 29984
Affinity Capture-MS Homo sapiens
109 HSPA4L 22824
Co-fractionation Homo sapiens
110 CAB39L 81617
Affinity Capture-MS Homo sapiens
111 EP300 2033
Affinity Capture-MS Homo sapiens
112 TAGAP  
Affinity Capture-MS Homo sapiens
113 GPAT2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PUS7 is involved
PathwayEvidenceSource
Metabolism of RNA IEA Reactome
tRNA modification in the nucleus and cytosol IEA Reactome
tRNA processing IEA Reactome





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