Gene description for NUP50
Gene name nucleoporin 50kDa
Gene symbol NUP50
Other names/aliases NPAP60
Species Homo sapiens
 Database cross references - NUP50
ExoCarta ExoCarta_10762
Entrez Gene 10762
HGNC 8065
MIM 604646
UniProt Q9UKX7  
 NUP50 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NUP50
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    gene expression GO:0010467 TAS
    carbohydrate metabolic process GO:0005975 TAS
    cellular protein metabolic process GO:0044267 TAS
    hexose transport GO:0008645 TAS
    mitotic nuclear envelope disassembly GO:0007077 TAS
    mRNA transport GO:0051028 IEA
    protein transport GO:0015031 IEA
    small molecule metabolic process GO:0044281 TAS
    regulation of cellular response to heat GO:1900034 TAS
    intracellular transport GO:0046907 IEA
    regulation of glucose transport GO:0010827 TAS
    viral process GO:0016032 TAS
    cellular response to heat GO:0034605 TAS
    viral transcription GO:0019083 TAS
    viral life cycle GO:0019058 TAS
    cytokine-mediated signaling pathway GO:0019221 TAS
    protein sumoylation GO:0016925 TAS
    post-translational protein modification GO:0043687 TAS
    mitotic cell cycle GO:0000278 TAS
    transmembrane transport GO:0055085 TAS
    glucose transport GO:0015758 TAS
Subcellular Localization
    nuclear membrane GO:0031965 IDA
    cytoplasm GO:0005737 IDA
    nucleoplasm GO:0005654 TAS
    nuclear pore GO:0005643 IEA
 Experiment description of studies that identified NUP50 in exosomes
Experiment ID 235
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NUP50
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KPNB1 3837
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 NUP153  
Affinity Capture-Western Homo sapiens
3 CDKN1B 1027
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
4 KPNA2 3838
Reconstituted Complex Homo sapiens
5 IPO13  
Reconstituted Complex Homo sapiens
6 XPO1 7514
Invitro Homo sapiens
Invivo Homo sapiens
View the network image/svg+xml

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