Gene description for ERO1L
Gene name ERO1-like (S. cerevisiae)
Gene symbol ERO1L
Other names/aliases ERO1-L
ERO1-L-alpha
ERO1-alpha
ERO1A
ERO1LA
Species Homo sapiens
 Database cross references - ERO1L
ExoCarta ExoCarta_30001
Entrez Gene 30001
HGNC 13280
MIM 615435
UniProt Q96HE7  
 ERO1L identified in exosomes derived from the following tissue/cell type
Bladder cancer cells 20224111    
Liver cancer cells 25265333    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for ERO1L
Molecular Function
    protein disulfide isomerase activity GO:0003756 IEA
    protein disulfide oxidoreductase activity GO:0015035 IEA
    oxidoreductase activity GO:0016491 IDA
    oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor GO:0016671 IEA
    protein binding GO:0005515 IPI
Biological Process
    oxidation-reduction process GO:0055114 IEA
    brown fat cell differentiation GO:0050873 IEA
    chaperone mediated protein folding requiring cofactor GO:0051085 IDA
    protein folding GO:0006457 TAS
    4-hydroxyproline metabolic process GO:0019471 IEA
    cell redox homeostasis GO:0045454 IEA
    response to endoplasmic reticulum stress GO:0034976 ISS
    cellular protein metabolic process GO:0044267 TAS
    endoplasmic reticulum unfolded protein response GO:0030968 IEA
    intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress GO:0070059 ISS
    cellular protein modification process GO:0006464 TAS
    release of sequestered calcium ion into cytosol GO:0051209 ISS
    response to reactive oxygen species GO:0000302 TAS
    protein maturation by protein folding GO:0022417 IEA
    extracellular matrix organization GO:0030198 IEA
    response to temperature stimulus GO:0009266 TAS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 IEA
    intracellular membrane-bounded organelle GO:0043231 TAS
    endoplasmic reticulum GO:0005783 IDA
    endoplasmic reticulum lumen GO:0005788 TAS
    membrane GO:0016020 IDA
 Experiment description of studies that identified ERO1L in exosomes
1
Experiment ID 76
ISEV standards
EM
EV Biophysical techniques
TSG101|GAPDH|HSP90
EV Cytosolic markers
CD81|CD9|CD63|LAMP1|MHCI
EV Membrane markers
HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
2
Experiment ID 267
ISEV standards
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EV Membrane markers
CANX|HSP90B1
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25265333    
Organism Homo sapiens
Experiment description Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh‑7 Cells Using SILAC Labeling and LC−MS/MS
Authors Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J
Journal name J Proteome Res
Publication year 2014
Sample Liver cancer cells
Sample name Huh-7 - Transfected with HBx plasmids
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ERO1L
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ERP44 23071
Invivo Homo sapiens
2 P4HB 5034
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which ERO1L is involved
PathwayEvidenceSource
Detoxification of Reactive Oxygen Species TAS Reactome
Insulin processing TAS Reactome





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