Gene description for NADK2
Gene name NAD kinase 2, mitochondrial
Gene symbol NADK2
Other names/aliases C5orf33
DECRD
MNADK
NADKD1
Species Homo sapiens
 Database cross references - NADK2
ExoCarta ExoCarta_133686
Vesiclepedia VP_133686
Entrez Gene 133686
HGNC 26404
MIM 615787
UniProt Q4G0N4  
 NADK2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for NADK2
Molecular Function
    NAD+ kinase activity GO:0003951 IBA
    NAD+ kinase activity GO:0003951 IDA
    ATP binding GO:0005524 IEA
    protein homodimerization activity GO:0042803 IDA
Biological Process
    NADP biosynthetic process GO:0006741 IEA
    NAD metabolic process GO:0019674 IBA
    NAD metabolic process GO:0019674 IDA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified NADK2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for NADK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MPST 4357
Co-fractionation Homo sapiens
2 CLPP 8192
Proximity Label-MS Homo sapiens
3 LYRM5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 ERMAP 114625
Co-fractionation Homo sapiens
5 DNAJB11 51726
Co-fractionation Homo sapiens
6 NPTX1  
Co-fractionation Homo sapiens
7 CHN1  
Co-fractionation Homo sapiens
8 KIF14 9928
Affinity Capture-MS Homo sapiens
9 HTRA2 27429
Co-fractionation Homo sapiens
10 MTPN 136319
Affinity Capture-MS Homo sapiens
11 FOLR1 2348
Affinity Capture-MS Homo sapiens
12 MDH2 4191
Proximity Label-MS Homo sapiens
13 RPA2 6118
Proximity Label-MS Homo sapiens
14 FERMT2 10979
Co-fractionation Homo sapiens
15 PNP 4860
Co-fractionation Homo sapiens
16 KIF20A 10112
Affinity Capture-MS Homo sapiens
17 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
18 PARK2  
Affinity Capture-MS Homo sapiens
19 TMEM177  
Co-fractionation Homo sapiens
20 IRF2BPL 64207
Co-fractionation Homo sapiens
21 VPS29 51699
Co-fractionation Homo sapiens
22 TIMM9  
Co-fractionation Homo sapiens
23 TMEM189  
Co-fractionation Homo sapiens
24 SUCLA2 8803
Co-fractionation Homo sapiens
25 STIM2 57620
Affinity Capture-MS Homo sapiens
26 STMN1 3925
Co-fractionation Homo sapiens
27 ACTB 60
Affinity Capture-MS Homo sapiens
28 MTX3  
Affinity Capture-MS Homo sapiens
29 SPECC1L 23384
Affinity Capture-MS Homo sapiens
30 RPA3 6119
Proximity Label-MS Homo sapiens
31 DYNLL1 8655
Co-fractionation Homo sapiens
32 ANPEP 290
Co-fractionation Homo sapiens
View the network image/svg+xml



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