Gene description for PLOD2
Gene name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
Gene symbol PLOD2
Other names/aliases BRKS2
LH2
TLH
Species Homo sapiens
 Database cross references - PLOD2
ExoCarta ExoCarta_5352
Entrez Gene 5352
HGNC 9082
MIM 601865
UniProt O00469  
 PLOD2 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Saliva 19199708    
 Gene ontology annotations for PLOD2
Molecular Function
    L-ascorbic acid binding GO:0031418 IEA
    iron ion binding GO:0005506 IEA
    procollagen-lysine 5-dioxygenase activity GO:0008475 IEA
Biological Process
    oxidation-reduction process GO:0055114 IEA
    response to hypoxia GO:0001666 IEP
    cellular protein modification process GO:0006464 TAS
    activation of mitophagy in response to mitochondrial depolarization GO:0098779 IMP
    extracellular matrix organization GO:0030198 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    endoplasmic reticulum membrane GO:0005789 TAS
    endoplasmic reticulum GO:0005783 TAS
    rough endoplasmic reticulum membrane GO:0030867 IEA
 Experiment description of studies that identified PLOD2 in exosomes
1
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
 Protein-protein interactions for PLOD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RUVBL2 10856
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PLOD2 is involved
PathwayEvidenceSource
Collagen biosynthesis and modifying enzymes TAS Reactome





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