Gene description for DUSP4
Gene name dual specificity phosphatase 4
Gene symbol DUSP4
Other names/aliases HVH2
MKP-2
MKP2
TYP
Species Homo sapiens
 Database cross references - DUSP4
ExoCarta ExoCarta_1846
Entrez Gene 1846
HGNC 3070
MIM 602747
UniProt Q13115  
 DUSP4 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for DUSP4
Molecular Function
    protein tyrosine/serine/threonine phosphatase activity GO:0008138 IBA
    MAP kinase tyrosine/serine/threonine phosphatase activity GO:0017017 IEA
    protein tyrosine/threonine phosphatase activity GO:0008330 TAS
    protein tyrosine phosphatase activity GO:0004725 IEA
Biological Process
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    inactivation of MAPK activity GO:0000188 IEA
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    MAPK cascade GO:0000165 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    protein dephosphorylation GO:0006470 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    peptidyl-tyrosine dephosphorylation GO:0035335 IEA
    innate immune response GO:0045087 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    stress-activated MAPK cascade GO:0051403 TAS
    endoderm formation GO:0001706 IBA
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    nucleus GO:0005634 TAS
 Experiment description of studies that identified DUSP4 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for DUSP4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK14 1432
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which DUSP4 is involved
PathwayEvidenceSource
ERKs are inactivated TAS Reactome
ERKs are inactivated TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here