Gene description for CRYAB
Gene name crystallin, alpha B
Gene symbol CRYAB
Other names/aliases CMD1II
CRYA2
CTPP2
CTRCT16
HEL-S-101
HSPB5
MFM2
Species Homo sapiens
 Database cross references - CRYAB
ExoCarta ExoCarta_1410
Entrez Gene 1410
HGNC 2389
MIM 123590
UniProt P02511  
 CRYAB identified in exosomes derived from the following tissue/cell type
Melanoma cells 25950383    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for CRYAB
Molecular Function
    unfolded protein binding GO:0051082 IPI
    identical protein binding GO:0042802 IPI
    structural constituent of eye lens GO:0005212 IEA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    microtubule binding GO:0008017 IEA
    protein homodimerization activity GO:0042803 IPI
Biological Process
    protein folding GO:0006457 IEA
    muscle organ development GO:0007517 IEA
    response to hypoxia GO:0001666 IEA
    negative regulation of apoptotic process GO:0043066 IDA
    stress-activated MAPK cascade GO:0051403 IEA
    protein homooligomerization GO:0051260 IDA
    negative regulation of cell growth GO:0030308 IEA
    muscle contraction GO:0006936 TAS
    aging GO:0007568 IEA
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IEA
    response to estradiol GO:0032355 IEA
    tubulin complex assembly GO:0007021 IEA
    microtubule polymerization or depolymerization GO:0031109 IEA
    response to hydrogen peroxide GO:0042542 IEA
    negative regulation of intracellular transport GO:0032387 IDA
    cellular response to gamma radiation GO:0071480 IMP
    negative regulation of gene expression GO:0010629 IEA
    lens development in camera-type eye GO:0002088 IEA
    negative regulation of reactive oxygen species metabolic process GO:2000378 IEA
    regulation of cell death GO:0010941 IMP
    apoptotic process involved in morphogenesis GO:0060561 IEA
Subcellular Localization
    Golgi apparatus GO:0005794 IEA
    extracellular exosome GO:0070062 IDA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 IEA
    mitochondrion GO:0005739 IEA
    cell surface GO:0009986 IEA
    microtubule cytoskeleton GO:0015630 IEA
    cytoplasm GO:0005737 IDA
    Z disc GO:0030018 IEA
    actin filament bundle GO:0032432 IEA
    nucleus GO:0005634 IDA
 Experiment description of studies that identified CRYAB in exosomes
1
Experiment ID 258
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
FLOT1|CD81
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name SKMEL28
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CRYAB
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRYAB 1410
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
2 CS 1431
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
3 PSMA3 5684
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
4 HSPB2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
5 BMPR2 659
Affinity Capture-MS Homo sapiens
6 HSPB1 3315
Invivo Homo sapiens
Invitro Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which CRYAB is involved
No pathways found





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