Gene description for GTF2A1
Gene name general transcription factor IIA, 1, 19/37kDa
Gene symbol GTF2A1
Other names/aliases TF2A1
TFIIA
TFIIA-42
TFIIAL
Species Homo sapiens
 Database cross references - GTF2A1
ExoCarta ExoCarta_2957
Vesiclepedia VP_2957
Entrez Gene 2957
HGNC 4646
MIM 600520
UniProt P52655  
 GTF2A1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for GTF2A1
Molecular Function
    RNA polymerase II core promoter sequence-specific DNA binding GO:0000979 IDA
    RNA polymerase II general transcription initiation factor binding GO:0001091 IPI
    DNA binding GO:0003677 IDA
    protein binding GO:0005515 IPI
    RNA polymerase II general transcription initiation factor activity GO:0016251 IDA
    RNA polymerase II general transcription initiation factor activity GO:0016251 TAS
    TBP-class protein binding GO:0017025 IPI
    protein heterodimerization activity GO:0046982 IPI
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IEA
Biological Process
    transcription by RNA polymerase II GO:0006366 IBA
    transcription by RNA polymerase II GO:0006366 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    RNA polymerase II preinitiation complex assembly GO:0051123 NAS
    positive regulation of transcription initiation by RNA polymerase II GO:0060261 IDA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    transcription factor TFIID complex GO:0005669 IDA
    transcription factor TFIIA complex GO:0005672 IBA
    transcription factor TFIIA complex GO:0005672 IDA
    transcription factor TFIIA complex GO:0005672 IPI
    cytosol GO:0005829 IDA
    transcription preinitiation complex GO:0097550 IDA
 Experiment description of studies that identified GTF2A1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for GTF2A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 RNF43  
Proximity Label-MS Homo sapiens
3 GTF2F1 2962
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
4 NCOA6  
Reconstituted Complex Homo sapiens
5 TERF2  
Affinity Capture-MS Homo sapiens
6 FAM129B 64855
Co-fractionation Homo sapiens
7 SARS 6301
Co-fractionation Homo sapiens
8 CUEDC2  
Affinity Capture-MS Homo sapiens
9 PRR20C  
Two-hybrid Homo sapiens
10 DNAJC8 22826
Proximity Label-MS Homo sapiens
11 PTCD2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TP53 7157
Co-fractionation Homo sapiens
13 PRR20A  
Two-hybrid Homo sapiens
14 PTMA 5757
Co-fractionation Homo sapiens
15 INPPL1 3636
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SP4  
Two-hybrid Homo sapiens
17 ZEB1  
Co-fractionation Homo sapiens
18 GARS 2617
Co-fractionation Homo sapiens
19 ATP6V1C1 528
Co-fractionation Homo sapiens
20 SP1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 TAF6 6878
Affinity Capture-MS Homo sapiens
22 BOP1 23246
Co-fractionation Homo sapiens
23 PRR20D  
Two-hybrid Homo sapiens
24 HMGB1 3146
Reconstituted Complex Homo sapiens
25 EGFR 1956
Negative Genetic Homo sapiens
26 TBPL1 9519
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 BCL3  
Reconstituted Complex Homo sapiens
28 SHMT2 6472
Affinity Capture-RNA Homo sapiens
29 DOCK7 85440
Affinity Capture-MS Homo sapiens
30 CREB1  
Two-hybrid Homo sapiens
31 SIRT1  
Co-fractionation Homo sapiens
32 SNX6 58533
Co-fractionation Homo sapiens
33 C17orf62 79415
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 TBCD 6904
Co-fractionation Homo sapiens
35 TAF5  
Affinity Capture-MS Homo sapiens
36 PRR20B  
Two-hybrid Homo sapiens
37 TBP  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 UGDH 7358
Co-fractionation Homo sapiens
39 NUP50 10762
Proximity Label-MS Homo sapiens
40 MYLK2 85366
Affinity Capture-MS Homo sapiens
41 RDX 5962
Co-fractionation Homo sapiens
42 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
43 MKX  
Affinity Capture-Western Homo sapiens
44 RPA3 6119
Proximity Label-MS Homo sapiens
45 GTF2E2  
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
46 KDM5B  
Affinity Capture-MS Homo sapiens
47 GORASP2 26003
Co-fractionation Homo sapiens
48 Tbp  
Affinity Capture-Western Mus musculus
49 BLMH 642
Co-fractionation Homo sapiens
50 GTF2E1 2960
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
51 GTF2A2 2958
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
52 PRR20E  
Two-hybrid Homo sapiens
53 TAF1  
Biochemical Activity Homo sapiens
54 BIRC7  
Two-hybrid Homo sapiens
55 CNOT1 23019
Affinity Capture-MS Homo sapiens
56 NFYA 4800
Two-hybrid Homo sapiens
57 RPUSD2  
Co-fractionation Homo sapiens
58 HSPA9 3313
Affinity Capture-MS Homo sapiens
59 TBPL2  
Affinity Capture-MS Homo sapiens
60 GCN1L1 10985
Affinity Capture-MS Homo sapiens
61 BCAT1 586
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 TAF4 6874
Affinity Capture-MS Homo sapiens
63 HUWE1 10075
Affinity Capture-MS Homo sapiens
64 PDIA6 10130
Co-fractionation Homo sapiens
65 ATG7 10533
Co-fractionation Homo sapiens
66 ME1 4199
Co-fractionation Homo sapiens
View the network image/svg+xml



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