Gene description for PNPT1
Gene name polyribonucleotide nucleotidyltransferase 1
Gene symbol PNPT1
Other names/aliases COXPD13
DFNB70
OLD35
PNPASE
old-35
Species Homo sapiens
 Database cross references - PNPT1
ExoCarta ExoCarta_87178
Vesiclepedia VP_87178
Entrez Gene 87178
HGNC 23166
MIM 610316
UniProt Q8TCS8  
 PNPT1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
T lymphocytes 34108659    
 Gene ontology annotations for PNPT1
Molecular Function
    3'-5'-RNA exonuclease activity GO:0000175 IBA
    3'-5'-RNA exonuclease activity GO:0000175 IDA
    3'-5'-RNA exonuclease activity GO:0000175 TAS
    RNA binding GO:0003723 HDA
    polyribonucleotide nucleotidyltransferase activity GO:0004654 IBA
    polyribonucleotide nucleotidyltransferase activity GO:0004654 IDA
    protein binding GO:0005515 IPI
    poly(U) RNA binding GO:0008266 IDA
    poly(G) binding GO:0034046 IDA
    miRNA binding GO:0035198 IDA
    identical protein binding GO:0042802 IPI
Biological Process
    mitochondrial RNA catabolic process GO:0000957 IDA
    mitochondrial RNA catabolic process GO:0000957 TAS
    mitochondrial mRNA catabolic process GO:0000958 IBA
    mitochondrial mRNA catabolic process GO:0000958 IDA
    positive regulation of mitochondrial RNA catabolic process GO:0000962 IDA
    mitochondrial RNA 5'-end processing GO:0000964 IMP
    mitochondrial RNA 3'-end processing GO:0000965 IBA
    mitochondrial RNA 3'-end processing GO:0000965 IMP
    mRNA processing GO:0006397 IEA
    RNA catabolic process GO:0006401 IDA
    mRNA catabolic process GO:0006402 IDA
    mitochondrion organization GO:0007005 ISS
    cellular response to oxidative stress GO:0034599 IDA
    cellular response to interferon-beta GO:0035458 IDA
    RNA import into mitochondrion GO:0035927 IDA
    rRNA import into mitochondrion GO:0035928 IDA
    regulation of cellular respiration GO:0043457 ISS
    negative regulation of growth GO:0045926 IDA
    protein homooligomerization GO:0051260 IDA
    response to cAMP GO:0051591 IEA
    regulation of cell cycle GO:0051726 IDA
    response to growth hormone GO:0060416 IEA
    positive regulation of mRNA catabolic process GO:0061014 IMP
    protein homotrimerization GO:0070207 IDA
    nuclear polyadenylation-dependent mRNA catabolic process GO:0071042 IDA
    mitochondrial mRNA polyadenylation GO:0097222 IMP
    liver regeneration GO:0097421 IEA
    positive regulation of miRNA catabolic process GO:2000627 IDA
    regulation of cellular senescence GO:2000772 IDA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial intermembrane space GO:0005758 IDA
    mitochondrial matrix GO:0005759 IDA
    mitochondrial matrix GO:0005759 TAS
    endoplasmic reticulum membrane GO:0005789 IEA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    ribosome GO:0005840 IEA
    mitochondrial degradosome GO:0045025 IDA
 Experiment description of studies that identified PNPT1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
8
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
9
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
11
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
12
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
13
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
14
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
15
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
16
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
17
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
18
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
20
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
 Protein-protein interactions for PNPT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 AMT  
Co-fractionation Homo sapiens
2 SLIRP 81892
Proximity Label-MS Homo sapiens
3 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
4 PMPCA 23203
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
5 CLIP1 6249
Affinity Capture-MS Homo sapiens
6 PTBP1 5725
Co-fractionation Homo sapiens
7 RIT1 6016
Negative Genetic Homo sapiens
8 HINT2 84681
Proximity Label-MS Homo sapiens
9 TRUB2  
Proximity Label-MS Homo sapiens
10 MTG2  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 EIF4A1 1973
Co-fractionation Homo sapiens
12 RPUSD4 84881
Proximity Label-MS Homo sapiens
13 CLTB 1212
Co-fractionation Homo sapiens
14 LTN1 26046
Affinity Capture-MS Homo sapiens
15 MTRF1  
Proximity Label-MS Homo sapiens
16 GFM2 84340
Proximity Label-MS Homo sapiens
17 LRPPRC 10128
Proximity Label-MS Homo sapiens
18 KIAA1429 25962
Affinity Capture-MS Homo sapiens
19 ATP5D 513
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 HEATR3 55027
Affinity Capture-MS Homo sapiens
21 TFAM 7019
Proximity Label-MS Homo sapiens
22 TMEM183A  
Affinity Capture-MS Homo sapiens
23 SUPV3L1 6832
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 NUP153 9972
Co-fractionation Homo sapiens
25 MRPL23 6150
Affinity Capture-MS Homo sapiens
26 YARS2  
Affinity Capture-MS Homo sapiens
27 TMED2 10959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 MRPL12 6182
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 FTSJ2 29960
Affinity Capture-MS Homo sapiens
30 ZSCAN31  
Affinity Capture-MS Homo sapiens
31 MRPS26 64949
Proximity Label-MS Homo sapiens
32 MALSU1  
Affinity Capture-MS Homo sapiens
33 ADARB1 104
Two-hybrid Homo sapiens
34 EXD2  
Proximity Label-MS Homo sapiens
35 SSBP1 6742
Proximity Label-MS Homo sapiens
36 MECP2 4204
Affinity Capture-MS Homo sapiens
37 PMPCB 9512
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
38 TACO1  
Proximity Label-MS Homo sapiens
39 FASTKD2  
Proximity Label-MS Homo sapiens
40 VWA8 23078
Proximity Label-MS Homo sapiens
41 YAF2  
Affinity Capture-MS Homo sapiens
42 PDLIM1 9124
Co-fractionation Homo sapiens
43 MTERF3  
Proximity Label-MS Homo sapiens
44 MDH2 4191
Proximity Label-MS Homo sapiens
45 SLC25A10 1468
Affinity Capture-MS Homo sapiens
46 C6orf203  
Proximity Label-MS Homo sapiens
47 FASTKD5  
Proximity Label-MS Homo sapiens
48 PARK2  
Affinity Capture-MS Homo sapiens
49 NCAPD2 9918
Co-fractionation Homo sapiens
50 TUFM 7284
Proximity Label-MS Homo sapiens
51 HSCB 150274
Proximity Label-MS Homo sapiens
52 HMGN1  
Co-fractionation Homo sapiens
53 NTRK1 4914
Affinity Capture-MS Homo sapiens
54 GFM1 85476
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
55 PPA2 27068
Affinity Capture-MS Homo sapiens
56 HMGCL 3155
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 MRPL11 65003
Proximity Label-MS Homo sapiens
58 MTRF1L  
Proximity Label-MS Homo sapiens
59 NCL 4691
Co-fractionation Homo sapiens
60 PCDHA4 56144
Affinity Capture-MS Homo sapiens
61 PDE12 201626
Affinity Capture-MS Homo sapiens
62 MRRF  
Proximity Label-MS Homo sapiens
63 MRPS12  
Proximity Label-MS Homo sapiens
64 MTIF2 4528
Proximity Label-MS Homo sapiens
65 CALR3  
Affinity Capture-MS Homo sapiens
66 SIRT7  
Affinity Capture-MS Homo sapiens
67 MTIF3  
Proximity Label-MS Homo sapiens
68 TCL1A  
Affinity Capture-MS Homo sapiens
69 FAHD1 81889
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 OSGEP 55644
Affinity Capture-MS Homo sapiens
71 NUP214 8021
Co-fractionation Homo sapiens
72 NXF1 10482
Affinity Capture-RNA Homo sapiens
73 AURKB 9212
Affinity Capture-MS Homo sapiens
74 GATC  
Co-fractionation Homo sapiens
75 C12orf65  
Proximity Label-MS Homo sapiens
76 MYCN  
Affinity Capture-MS Homo sapiens
77 PRR3  
Affinity Capture-MS Homo sapiens
78 MTG1  
Proximity Label-MS Homo sapiens
79 ACAD9 28976
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 KRAS 3845
Negative Genetic Homo sapiens
81 MRPS17 51373
Affinity Capture-MS Homo sapiens
82 METTL15  
Proximity Label-MS Homo sapiens
83 PFDN5 5204
Affinity Capture-MS Homo sapiens
84 CLPP 8192
Proximity Label-MS Homo sapiens
85 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
86 MRPL42  
Affinity Capture-MS Homo sapiens
87 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
88 PRKCSH 5589
Co-fractionation Homo sapiens
89 LRFN2  
Affinity Capture-MS Homo sapiens
90 TSFM 10102
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
91 AUH 549
Proximity Label-MS Homo sapiens
92 SERPINA12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 ICT1 3396
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
94 LONP1 9361
Proximity Label-MS Homo sapiens
95 DPY30 84661
Co-fractionation Homo sapiens
96 PDHA1 5160
Proximity Label-MS Homo sapiens
97 TRMT61B  
Affinity Capture-MS Homo sapiens
98 HSPB1 3315
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
99 COX8A  
Proximity Label-MS Homo sapiens
100 SND1 27044
Co-fractionation Homo sapiens
101 C21orf33  
Proximity Label-MS Homo sapiens
102 WIF1 11197
Affinity Capture-MS Homo sapiens
103 FASTKD3  
Proximity Label-MS Homo sapiens
104 NUP107 57122
Co-fractionation Homo sapiens
105 BCL2L13 23786
Co-fractionation Homo sapiens
106 TBRG4 9238
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 AARS2  
Proximity Label-MS Homo sapiens
108 CIART  
Affinity Capture-MS Homo sapiens
109 GRSF1 2926
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
110 C1QBP 708
Proximity Label-MS Homo sapiens
111 SERBP1 26135
Affinity Capture-MS Homo sapiens
112 QRSL1  
Affinity Capture-MS Homo sapiens
113 METTL17  
Proximity Label-MS Homo sapiens
114 NVL  
Affinity Capture-MS Homo sapiens
115 RANGAP1 5905
Co-fractionation Homo sapiens
116 DDX28  
Proximity Label-MS Homo sapiens
117 EP300 2033
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PNPT1 is involved
PathwayEvidenceSource
Metabolism of RNA TAS Reactome
Mitochondrial RNA degradation TAS Reactome





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