Gene description for IARS2
Gene name isoleucyl-tRNA synthetase 2, mitochondrial
Gene symbol IARS2
Other names/aliases CAGSSS
ILERS
Species Homo sapiens
 Database cross references - IARS2
ExoCarta ExoCarta_55699
Vesiclepedia VP_55699
Entrez Gene 55699
HGNC 29685
MIM 612801
UniProt Q9NSE4  
 IARS2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
T lymphocytes 34108659    
 Gene ontology annotations for IARS2
Molecular Function
    tRNA binding GO:0000049 IEA
    aminoacyl-tRNA editing activity GO:0002161 IEA
    isoleucine-tRNA ligase activity GO:0004822 IBA
    isoleucine-tRNA ligase activity GO:0004822 TAS
    ATP binding GO:0005524 IEA
Biological Process
    tRNA aminoacylation for protein translation GO:0006418 TAS
    isoleucyl-tRNA aminoacylation GO:0006428 IBA
    mitochondrial translation GO:0032543 IBA
    aminoacyl-tRNA metabolism involved in translational fidelity GO:0106074 IEA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrial matrix GO:0005759 TAS
 Experiment description of studies that identified IARS2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
15
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
16
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
17
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
18
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
20
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
21
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
22
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
25
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
27
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
28
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
 Protein-protein interactions for IARS2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Co-fractionation Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 SLIRP 81892
Proximity Label-MS Homo sapiens
4 UQCRFS1 7386
Affinity Capture-MS Homo sapiens
5 DCTN2 10540
Proximity Label-MS Homo sapiens
6 Cul1 26965
Affinity Capture-MS Mus musculus
7 SPRTN  
Affinity Capture-MS Homo sapiens
8 FANCA  
Affinity Capture-MS Homo sapiens
9 CLIP1 6249
Proximity Label-MS Homo sapiens
10 STAT2 6773
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 ACSM5 54988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 GJA1 2697
Proximity Label-MS Homo sapiens
13 Iqgap3 404710
Affinity Capture-MS Mus musculus
14 YWHAE 7531
Co-fractionation Homo sapiens
15 Nek2  
Affinity Capture-MS Mus musculus
16 Papss1  
Affinity Capture-MS Mus musculus
17 TRUB2  
Proximity Label-MS Homo sapiens
18 UBA1 7317
Co-fractionation Homo sapiens
19 MTG2  
Proximity Label-MS Homo sapiens
20 NGRN  
Proximity Label-MS Homo sapiens
21 CHCHD1  
Proximity Label-MS Homo sapiens
22 HSD17B11 51170
Proximity Label-MS Homo sapiens
23 HSP90AB1 3326
Affinity Capture-MS Homo sapiens
24 NDUFS7 374291
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 RPUSD4 84881
Proximity Label-MS Homo sapiens
26 DNAJC15  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
27 MTRF1  
Proximity Label-MS Homo sapiens
28 GFM2 84340
Proximity Label-MS Homo sapiens
29 CCDC90B  
Proximity Label-MS Homo sapiens
30 ILF2 3608
Co-fractionation Homo sapiens
31 LRPPRC 10128
Proximity Label-MS Homo sapiens
32 WDR62  
Affinity Capture-MS Homo sapiens
33 PARK2  
Affinity Capture-MS Homo sapiens
34 HEATR3 55027
Affinity Capture-MS Homo sapiens
35 MCU 90550
Proximity Label-MS Homo sapiens
36 CRYZ 1429
Proximity Label-MS Homo sapiens
37 TFAM 7019
Proximity Label-MS Homo sapiens
38 DNAJC19 131118
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
39 PMPCA 23203
Proximity Label-MS Homo sapiens
40 C8orf82  
Proximity Label-MS Homo sapiens
41 ACTR3 10096
Proximity Label-MS Homo sapiens
42 CDH2 1000
Co-fractionation Homo sapiens
43 PAFAH2 5051
Co-fractionation Homo sapiens
44 HINT2 84681
Proximity Label-MS Homo sapiens
45 CALD1 800
Affinity Capture-MS Homo sapiens
46 PFDN4 5203
Co-fractionation Homo sapiens
47 YWHAG 7532
Co-fractionation Homo sapiens
48 NARS 4677
Co-fractionation Homo sapiens
49 RER1 11079
Affinity Capture-MS Homo sapiens
50 GAK 2580
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 MRPS26 64949
Proximity Label-MS Homo sapiens
52 SURF1  
Proximity Label-MS Homo sapiens
53 EXD2  
Proximity Label-MS Homo sapiens
54 CHORDC1 26973
Affinity Capture-MS Homo sapiens
55 ASPM 259266
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 YWHAH 7533
Co-fractionation Homo sapiens
57 ESPL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 NAA38  
Affinity Capture-MS Homo sapiens
59 RNF126  
Affinity Capture-MS Homo sapiens
60 PMPCB 9512
Proximity Label-MS Homo sapiens
61 TACO1  
Proximity Label-MS Homo sapiens
62 FASTKD2  
Proximity Label-MS Homo sapiens
63 VWA8 23078
Proximity Label-MS Homo sapiens
64 PFDN5 5204
Affinity Capture-MS Homo sapiens
65 RAD23A 5886
Co-fractionation Homo sapiens
66 KIF14 9928
Affinity Capture-MS Homo sapiens
67 MTO1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 MDH2 4191
Proximity Label-MS Homo sapiens
69 CHMP4C 92421
Affinity Capture-MS Homo sapiens
70 GFAP 2670
Proximity Label-MS Homo sapiens
71 C6orf203  
Proximity Label-MS Homo sapiens
72 COX15 1355
Proximity Label-MS Homo sapiens
73 FASTKD5  
Proximity Label-MS Homo sapiens
74 PRPH 5630
Proximity Label-MS Homo sapiens
75 PEX3 8504
Proximity Label-MS Homo sapiens
76 ARRDC3 57561
Affinity Capture-MS Homo sapiens
77 QPRT 23475
Co-fractionation Homo sapiens
78 MALSU1  
Affinity Capture-MS Homo sapiens
79 CUL3 8452
Affinity Capture-MS Homo sapiens
80 KLC4 89953
Affinity Capture-MS Homo sapiens
81 PPT2  
Co-fractionation Homo sapiens
82 DDX11 1663
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 TUFM 7284
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
84 TMEM25  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 DIABLO 56616
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
86 HSCB 150274
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
87 RPA2 6118
Proximity Label-MS Homo sapiens
88 MYH9 4627
Affinity Capture-MS Homo sapiens
89 NTRK1 4914
Affinity Capture-MS Homo sapiens
90 CCDC109B 55013
Proximity Label-MS Homo sapiens
91 TBRG4 9238
Proximity Label-MS Homo sapiens
92 FH 2271
Co-fractionation Homo sapiens
93 DHX40  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 MTFMT  
Proximity Label-MS Homo sapiens
95 MRPL11 65003
Proximity Label-MS Homo sapiens
96 MTRF1L  
Proximity Label-MS Homo sapiens
97 DNAJC30  
Proximity Label-MS Homo sapiens
98 TMEM70  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 ALDH2 217
Co-fractionation Homo sapiens
100 DRG2 1819
Affinity Capture-MS Homo sapiens
101 FOXS1  
Affinity Capture-MS Homo sapiens
102 COX4I1 1327
Proximity Label-MS Homo sapiens
103 DNAJC7 7266
Proximity Label-MS Homo sapiens
104 IFT122 55764
Affinity Capture-MS Homo sapiens
105 OTC 5009
Proximity Label-MS Homo sapiens
106 HYAL4  
Co-fractionation Homo sapiens
107 MRPL21 219927
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 MRRF  
Proximity Label-MS Homo sapiens
109 AIFM1 9131
Proximity Label-MS Homo sapiens
110 MRPS12  
Proximity Label-MS Homo sapiens
111 MTIF2 4528
Proximity Label-MS Homo sapiens
112 UGGT1 56886
Affinity Capture-MS Homo sapiens
113 PANX1 24145
Proximity Label-MS Homo sapiens
114 SMG8 55181
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
115 CLPP 8192
Proximity Label-MS Homo sapiens
116 SIRT7  
Affinity Capture-MS Homo sapiens
117 MTIF3  
Proximity Label-MS Homo sapiens
118 HSFX1  
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
119 ATG12  
Proximity Label-MS Homo sapiens
120 ASS1 445
Affinity Capture-MS Homo sapiens
121 RMND1 55005
Proximity Label-MS Homo sapiens
122 HOOK3 84376
Proximity Label-MS Homo sapiens
123 ARF6 382
Proximity Label-MS Homo sapiens
124 DHX30 22907
Proximity Label-MS Homo sapiens
125 CELF5  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
126 VBP1 7411
Co-fractionation Homo sapiens
127 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
128 EPHA7 2045
Affinity Capture-MS Homo sapiens
129 C12orf65  
Proximity Label-MS Homo sapiens
130 MAPRE3  
Proximity Label-MS Homo sapiens
131 FKBP5 2289
Affinity Capture-MS Homo sapiens
132 EPHA2 1969
Proximity Label-MS Homo sapiens
133 MTG1  
Proximity Label-MS Homo sapiens
134 RPA3 6119
Proximity Label-MS Homo sapiens
135 YWHAQ 10971
Reconstituted Complex Homo sapiens
136 HNRNPH2 3188
Co-fractionation Homo sapiens
137 ACAD9 28976
Proximity Label-MS Homo sapiens
138 MATR3 9782
Co-fractionation Homo sapiens
139 Bub1b 12236
Affinity Capture-MS Mus musculus
140 CLPB 81570
Proximity Label-MS Homo sapiens
141 HSPA9 3313
Proximity Label-MS Homo sapiens
142 METTL15  
Proximity Label-MS Homo sapiens
143 AKAP1 8165
Proximity Label-MS Homo sapiens
144 MCUR1 63933
Proximity Label-MS Homo sapiens
145 FLOT1 10211
Proximity Label-MS Homo sapiens
146 APP 351
Reconstituted Complex Homo sapiens
147 DNAJC4  
Proximity Label-MS Homo sapiens
148 MTERF3  
Proximity Label-MS Homo sapiens
149 DNAJC28  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
150 CS 1431
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
151 C17orf80 55028
Proximity Label-MS Homo sapiens
152 PHB2 11331
Proximity Label-MS Homo sapiens
153 TSFM 10102
Proximity Label-MS Homo sapiens
154 AUH 549
Proximity Label-MS Homo sapiens
155 LAMTOR1 55004
Proximity Label-MS Homo sapiens
156 ILF3 3609
Co-fractionation Homo sapiens
157 JUP 3728
Co-fractionation Homo sapiens
158 TP53 7157
Affinity Capture-MS Homo sapiens
159 ICT1 3396
Proximity Label-MS Homo sapiens
160 DNAJA2 10294
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
161 CALU 813
Co-fractionation Homo sapiens
162 EPHA4 2043
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
163 DNA2  
Affinity Capture-MS Homo sapiens
164 ALDH1B1 219
Co-fractionation Homo sapiens
165 PDHA1 5160
Proximity Label-MS Homo sapiens
166 DNAJA3 9093
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
167 SQSTM1 8878
Proximity Label-MS Homo sapiens
168 HSPB1 3315
Two-hybrid Homo sapiens
169 GRPEL1 80273
Co-fractionation Homo sapiens
170 COX8A  
Proximity Label-MS Homo sapiens
171 CAPNS1 826
Co-fractionation Homo sapiens
172 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
173 MTHFD1 4522
Co-fractionation Homo sapiens
174 C21orf33  
Proximity Label-MS Homo sapiens
175 SSBP1 6742
Proximity Label-MS Homo sapiens
176 YARS2  
Affinity Capture-MS Homo sapiens
177 SFXN1 94081
Proximity Label-MS Homo sapiens
178 UFL1 23376
Affinity Capture-MS Homo sapiens
179 ASB8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
180 FASTKD3  
Proximity Label-MS Homo sapiens
181 RNASEH2A 10535
Affinity Capture-MS Homo sapiens
182 MBNL1 4154
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
183 LONP1 9361
Proximity Label-MS Homo sapiens
184 GFM1 85476
Proximity Label-MS Homo sapiens
185 AARS2  
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
186 PARD6B 84612
Affinity Capture-MS Homo sapiens
187 HNRNPH3 3189
Co-fractionation Homo sapiens
188 TEFM  
Proximity Label-MS Homo sapiens
189 PINK1  
Affinity Capture-MS Homo sapiens
190 RPUSD3  
Proximity Label-MS Homo sapiens
191 GRSF1 2926
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
192 C1QBP 708
Proximity Label-MS Homo sapiens
193 SERBP1 26135
Affinity Capture-MS Homo sapiens
194 KRAS 3845
Negative Genetic Homo sapiens
195 C9orf72  
Affinity Capture-MS Homo sapiens
196 METTL17  
Proximity Label-MS Homo sapiens
197 YBEY  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
198 BBS1 582
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
199 ECSIT 51295
Affinity Capture-MS Homo sapiens
200 DERL1 79139
Proximity Label-MS Homo sapiens
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