Gene description for PPIP5K1
Gene name diphosphoinositol pentakisphosphate kinase 1
Gene symbol PPIP5K1
Other names/aliases HISPPD2A
IP6K
IPS1
VIP1
hsVIP1
Species Homo sapiens
 Database cross references - PPIP5K1
ExoCarta ExoCarta_9677
Vesiclepedia VP_9677
Entrez Gene 9677
HGNC 29023
MIM 610979
UniProt Q6PFW1  
 PPIP5K1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PPIP5K1
Molecular Function
    inositol-1,3,4,5,6-pentakisphosphate kinase activity GO:0000827 IDA
    inositol hexakisphosphate kinase activity GO:0000828 IBA
    inositol hexakisphosphate kinase activity GO:0000828 IDA
    inositol hexakisphosphate kinase activity GO:0000828 TAS
    diphosphoinositol pentakisphosphate kinase activity GO:0000829 IBA
    diphosphoinositol pentakisphosphate kinase activity GO:0000829 TAS
    inositol hexakisphosphate 5-kinase activity GO:0000832 IDA
    ATP binding GO:0005524 ISS
    5-diphosphoinositol pentakisphosphate 1-kinase activity GO:0033857 IBA
    5-diphosphoinositol pentakisphosphate 1-kinase activity GO:0033857 IDA
    inositol hexakisphosphate 1-kinase activity GO:0052723 IEA
Biological Process
    inositol metabolic process GO:0006020 IBA
    inositol metabolic process GO:0006020 IDA
    inositol phosphate biosynthetic process GO:0032958 IBA
    inositol phosphate metabolic process GO:0043647 TAS
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
 Experiment description of studies that identified PPIP5K1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PPIP5K1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 NT5C 30833
Affinity Capture-MS Homo sapiens
3 NFKBIB  
Affinity Capture-MS Homo sapiens
4 CIB2  
Affinity Capture-MS Homo sapiens
5 NSUN5P1  
Affinity Capture-MS Homo sapiens
6 ZBTB8B  
Co-fractionation Homo sapiens
7 ZHX1-C8orf76  
Affinity Capture-MS Homo sapiens
8 SWSAP1  
Affinity Capture-MS Homo sapiens
9 MIF4GD 57409
Co-fractionation Homo sapiens
10 C9orf163  
Affinity Capture-MS Homo sapiens
11 DHDH  
Affinity Capture-MS Homo sapiens
12 FAM167A  
Affinity Capture-MS Homo sapiens
13 SPHK1 8877
Affinity Capture-MS Homo sapiens
14 EIF6 3692
Proximity Label-MS Homo sapiens
15 VAC14 55697
Co-fractionation Homo sapiens
16 WBSCR22  
Co-fractionation Homo sapiens
17 MYH14 79784
Affinity Capture-MS Homo sapiens
18 FAM96A  
Affinity Capture-MS Homo sapiens
19 HDHD1  
Affinity Capture-MS Homo sapiens
20 CUL3 8452
Affinity Capture-MS Homo sapiens
21 KIF20A 10112
Affinity Capture-MS Homo sapiens
22 LSM4 25804
Co-fractionation Homo sapiens
23 MAP2K2 5605
Affinity Capture-MS Homo sapiens
24 METTL21B  
Affinity Capture-MS Homo sapiens
25 DUSP16  
Affinity Capture-MS Homo sapiens
26 TXNDC9 10190
Co-fractionation Homo sapiens
27 CRYBB3  
Affinity Capture-MS Homo sapiens
28 DHFR 1719
Affinity Capture-MS Homo sapiens
29 ANKRD13A 88455
Affinity Capture-MS Homo sapiens
30 INF2 64423
Affinity Capture-MS Homo sapiens
31 C12orf74  
Affinity Capture-MS Homo sapiens
32 HMGN1  
Co-fractionation Homo sapiens
33 RFXANK  
Affinity Capture-MS Homo sapiens
34 PPARD  
Affinity Capture-MS Homo sapiens
35 HES6  
Affinity Capture-MS Homo sapiens
36 ALDH3B1 221
Affinity Capture-MS Homo sapiens
37 NPRL2  
Affinity Capture-MS Homo sapiens
38 SF3B3 23450
Affinity Capture-MS Homo sapiens
39 YWHAG 7532
Affinity Capture-MS Homo sapiens
40 IRF3 3661
Affinity Capture-Western Homo sapiens
41 ZC3H15 55854
Co-fractionation Homo sapiens
42 CLPB 81570
Affinity Capture-MS Homo sapiens
43 HSPB8 26353
Affinity Capture-MS Homo sapiens
44 CYLD  
Affinity Capture-Western Homo sapiens
45 UBXN6 80700
Affinity Capture-MS Homo sapiens
46 UPP1 7378
Affinity Capture-MS Homo sapiens
47 ATG7 10533
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PPIP5K1 is involved
PathwayEvidenceSource
Inositol phosphate metabolism TAS Reactome
Metabolism TAS Reactome
Synthesis of pyrophosphates in the cytosol TAS Reactome





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