Gene description for SORBS1
Gene name
sorbin and SH3 domain containing 1
Gene symbol
SORBS1
Other names/aliases
CAP FLAF2 R85FL SH3D5 SH3P12 SORB1
Species
Homo sapiens
Database cross references - SORBS1
ExoCarta
ExoCarta_10580
Entrez Gene
10580
HGNC
14565
MIM
605264
UniProt
Q9BX66
SORBS1 identified in exosomes derived from the following tissue/cell type
Hepatocytes
26054723
Neuroblastoma cells
25944692
Gene ontology annotations for SORBS1
Experiment description of studies that identified SORBS1 in exosomes
1
Experiment ID
237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocytes
Sample name
MIHA
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RNA Sequencing
2
Experiment ID
224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
Protein-protein interactions for SORBS1
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
CBL
867
Affinity Capture-Western
Homo sapiens
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
2
INSM1
Invivo
Homo sapiens
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
3
MLLT4
4301
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
4
ABL1
25
Invivo
Homo sapiens
Invitro
Homo sapiens
Affinity Capture-Western
Homo sapiens
5
INSR
3643
Invivo
Homo sapiens
Invitro
Homo sapiens
6
FLOT1
Invitro
Homo sapiens
Affinity Capture-Western
Homo sapiens
7
VCL
7414
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
8
PTK2B
2185
Affinity Capture-Western
Homo sapiens
9
EIF6
3692
Affinity Capture-MS
Homo sapiens
10
INPPL1
3636
Invivo
Homo sapiens
Invitro
Homo sapiens
Two-hybrid
Homo sapiens
View the network
image/svg+xml
Pathways in which SORBS1 is involved