Gene description for WRNIP1
Gene name Werner helicase interacting protein 1
Gene symbol WRNIP1
Other names/aliases WHIP
bA420G6.2
Species Homo sapiens
 Database cross references - WRNIP1
ExoCarta ExoCarta_56897
Vesiclepedia VP_56897
Entrez Gene 56897
HGNC 20876
MIM 608196
UniProt Q96S55  
 WRNIP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for WRNIP1
Molecular Function
    DNA binding GO:0003677 IEA
    DNA clamp loader activity GO:0003689 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    enzyme activator activity GO:0008047 IBA
    ATP hydrolysis activity GO:0016887 IMP
    single-stranded DNA helicase activity GO:0017116 IBA
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
    cohesin loader activity GO:0061775 IEA
    chromatin extrusion motor activity GO:0140584 IEA
    ATP-dependent H3-H4 histone complex chaperone activity GO:0140665 IEA
    ATP-dependent H2AZ histone chaperone activity GO:0140849 IEA
Biological Process
    DNA synthesis involved in DNA repair GO:0000731 IBA
    DNA synthesis involved in DNA repair GO:0000731 IDA
    DNA-templated DNA replication GO:0006261 IBA
    chromatin remodeling GO:0006338 IEA
    regulation of DNA-templated DNA replication initiation GO:0030174 IDA
    DNA duplex unwinding GO:0032508 IEA
    innate immune response GO:0045087 IEA
    chromatin looping GO:0140588 IEA
Subcellular Localization
    chromosome, telomeric region GO:0000781 IDA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISS
    membrane GO:0016020 HDA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified WRNIP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for WRNIP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KDF1  
Affinity Capture-MS Homo sapiens
2 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
3 DZIP3  
Affinity Capture-MS Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 OSBPL10  
Affinity Capture-MS Homo sapiens
6 FOXA3  
Affinity Capture-MS Homo sapiens
7 SIRT6  
Affinity Capture-MS Homo sapiens
8 PARP1 142
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
9 FOXP3  
Affinity Capture-MS Homo sapiens
10 USP25  
Affinity Capture-MS Homo sapiens
11 METTL14  
Affinity Capture-MS Homo sapiens
12 USP37  
Proximity Label-MS Homo sapiens
13 WWP1 11059
Affinity Capture-MS Homo sapiens
14 XPO1 7514
Affinity Capture-MS Homo sapiens
15 WRN 7486
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
16 EPAS1  
Affinity Capture-MS Homo sapiens
17 SRSF4 6429
Co-fractionation Homo sapiens
18 RNF31 55072
Affinity Capture-MS Homo sapiens
19 TOLLIP 54472
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
20 POLD1 5424
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 ACTC1 70
Proximity Label-MS Homo sapiens
22 ELP3 55140
Co-fractionation Homo sapiens
23 CUL3 8452
Affinity Capture-MS Homo sapiens
24 WRNIP1 56897
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 ABL1 25
Biochemical Activity Homo sapiens
26 UBC 7316
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
27 USP11 8237
Affinity Capture-Western Homo sapiens
28 PARK2  
Affinity Capture-MS Homo sapiens
29 TAF1  
Affinity Capture-MS Homo sapiens
30 RPS6KB2  
Affinity Capture-MS Homo sapiens
31 ELP2 55250
Co-fractionation Homo sapiens
32 ATMIN  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
33 RPA4  
Proximity Label-MS Homo sapiens
34 PCNA 5111
Reconstituted Complex Homo sapiens
35 CBX3 11335
Proximity Label-MS Homo sapiens
36 C9orf78 51759
Affinity Capture-MS Homo sapiens
37 NUP50 10762
Proximity Label-MS Homo sapiens
38 NUP107 57122
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 PIN1 5300
Reconstituted Complex Homo sapiens
40 ADIPOQ 9370
Affinity Capture-MS Homo sapiens
41 MYO6 4646
Proximity Label-MS Homo sapiens
42 RPA3 6119
Proximity Label-MS Homo sapiens
43 VCP 7415
Affinity Capture-MS Homo sapiens
44 C16orf72 29035
Affinity Capture-MS Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 PINK1  
Affinity Capture-MS Homo sapiens
47 TRIM28 10155
Co-fractionation Homo sapiens
48 RAD18  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
49 KDM4B  
Affinity Capture-MS Homo sapiens
50 METTL3  
Affinity Capture-MS Homo sapiens
51 BRD1 23774
Affinity Capture-MS Homo sapiens
52 SMAD2 4087
Affinity Capture-MS Homo sapiens
53 Uba52 22186
Co-crystal Structure Mus musculus
54 OTULIN 90268
Affinity Capture-MS Homo sapiens
55 PML 5371
Affinity Capture-MS Homo sapiens
56 USP49 25862
Affinity Capture-MS Homo sapiens
57 AR 367
Proximity Label-MS Homo sapiens
58 DDX58 23586
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
59 LMNA 4000
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
60 CEBPA  
Protein-peptide Homo sapiens
61 IRF3 3661
Affinity Capture-MS Homo sapiens
62 COIL  
Proximity Label-MS Homo sapiens
63 FGFR1OP  
Affinity Capture-Western Homo sapiens
64 HUWE1 10075
Affinity Capture-MS Homo sapiens
65 CDC5L 988
Affinity Capture-MS Homo sapiens
66 ATRX 546
Affinity Capture-MS Homo sapiens
67 SLX4  
Affinity Capture-MS Homo sapiens
68 C9orf72  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which WRNIP1 is involved
No pathways found





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