Gene description for HSD3B2
Gene name hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
Gene symbol HSD3B2
Other names/aliases HSD3B
HSDB
SDR11E2
Species Homo sapiens
 Database cross references - HSD3B2
ExoCarta ExoCarta_3284
Entrez Gene 3284
HGNC 5218
MIM 613890
UniProt P26439  
 HSD3B2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for HSD3B2
Molecular Function
    3-beta-hydroxy-delta5-steroid dehydrogenase activity GO:0003854 IDA
    steroid delta-isomerase activity GO:0004769 IDA
Biological Process
    glucocorticoid biosynthetic process GO:0006704 TAS
    androgen biosynthetic process GO:0006702 TAS
    small molecule metabolic process GO:0044281 TAS
    steroid metabolic process GO:0008202 TAS
    oxidation-reduction process GO:0055114 IEA
    mineralocorticoid biosynthetic process GO:0006705 TAS
    steroid biosynthetic process GO:0006694 IDA
Subcellular Localization
    endoplasmic reticulum GO:0005783 NAS
    smooth endoplasmic reticulum membrane GO:0030868 ISS
    mitochondrial intermembrane space GO:0005758 ISS
    mitochondrial inner membrane GO:0005743 ISS
    mitochondrial membrane GO:0031966 NAS
    endoplasmic reticulum membrane GO:0005789 TAS
    integral component of membrane GO:0016021 NAS
 Experiment description of studies that identified HSD3B2 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for HSD3B2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which HSD3B2 is involved
PathwayEvidenceSource
Androgen biosynthesis TAS Reactome
Glucocorticoid biosynthesis TAS Reactome
Mineralocorticoid biosynthesis TAS Reactome





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