Gene description for CYFIP2
Gene name cytoplasmic FMR1 interacting protein 2
Gene symbol CYFIP2
Other names/aliases PIR121
Species Homo sapiens
 Database cross references - CYFIP2
ExoCarta ExoCarta_26999
Vesiclepedia VP_26999
Entrez Gene 26999
HGNC 13760
MIM 606323
UniProt Q96F07  
 CYFIP2 identified in sEVs derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Embryonic kidney cells 36706192    
Embryonic kidney cells 36706192    
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Thymus 23844026    
Urine 19056867    
 Gene ontology annotations for CYFIP2
Molecular Function
    RNA 7-methylguanosine cap binding GO:0000340 IBA
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IEA
Biological Process
    cell morphogenesis GO:0000902 IBA
    apoptotic process GO:0006915 IBA
    apoptotic process GO:0006915 IDA
    axon guidance GO:0007411 IBA
    cell projection assembly GO:0030031 IBA
    regulation of actin filament polymerization GO:0030833 IEA
    positive regulation of proteolysis GO:0045862 IDA
    positive regulation of neurotrophin TRK receptor signaling pathway GO:0051388 IEA
    activation of cysteine-type endopeptidase activity GO:0097202 IDA
    dendrite extension GO:0097484 IEA
    cell-cell adhesion GO:0098609 IDA
    regulation of postsynapse assembly GO:0150052 IDA
    regulation of postsynapse assembly GO:0150052 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    SCAR complex GO:0031209 IDA
    neuron projection GO:0043005 IBA
    synapse GO:0045202 IBA
    synapse GO:0045202 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified CYFIP2 in sEVs
1
Experiment ID 79
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
MISEV standards
✘ Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors "Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
5
Experiment ID 1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36706192    
Organism Homo sapiens
Experiment description Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors "Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name Sci Adv
Publication year 2023
Sample Embryonic kidney cells
Sample name HEK293T - Fraction 5
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density 1.151 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for CYFIP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 HOMER3 9454
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 MIB1 57534
Proximity Label-MS Homo sapiens
4 PACSIN1  
Affinity Capture-MS Homo sapiens
5 FAM167A  
Affinity Capture-MS Homo sapiens
6 RIN3  
Affinity Capture-MS Homo sapiens
7 WASF2 10163
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
8 FMR1 2332
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
9 MCM3 4172
Co-fractionation Homo sapiens
10 ACTC1 70
Proximity Label-MS Homo sapiens
11 PACSIN2 11252
Affinity Capture-MS Homo sapiens
12 TYROBP  
Affinity Capture-MS Homo sapiens
13 ZC3H12A  
Two-hybrid Homo sapiens
14 APBB1  
Reconstituted Complex Homo sapiens
15 PRPF40A 55660
Reconstituted Complex Homo sapiens
16 PLG 5340
Two-hybrid Homo sapiens
17 VASP 7408
Affinity Capture-MS Homo sapiens
18 CAPZB 832
Affinity Capture-MS Homo sapiens
19 NCKAP1L 3071
Co-fractionation Homo sapiens
20 HLA-B 3106
Affinity Capture-MS Homo sapiens
21 ABI1 10006
Co-fractionation Homo sapiens
22 BIRC3 330
Two-hybrid Homo sapiens
23 YWHAH 7533
Affinity Capture-MS Homo sapiens
24 ATG16L1 55054
Affinity Capture-MS Homo sapiens
25 SFN 2810
Co-fractionation Homo sapiens
26 LHX4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 MARK4  
Affinity Capture-MS Homo sapiens
28 TIMM50 92609
Affinity Capture-MS Homo sapiens
29 MYCN  
Affinity Capture-MS Homo sapiens
30 BRK1 55845
Affinity Capture-MS Homo sapiens
31 NCK1 4690
Affinity Capture-MS Homo sapiens
32 WASF3 10810
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
33 MCAM 4162
Proximity Label-MS Homo sapiens
34 CGB2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 HSPA8 3312
Affinity Capture-MS Homo sapiens
36 YWHAB 7529
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
37 ARHGAP12  
Affinity Capture-MS Homo sapiens
38 HS2ST1 9653
Affinity Capture-MS Homo sapiens
39 IKBIP 121457
Affinity Capture-MS Homo sapiens
40 C19orf38  
Affinity Capture-MS Homo sapiens
41 WASF1 8936
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
42 P2RY8  
Affinity Capture-MS Homo sapiens
43 STAU1 6780
Affinity Capture-MS Homo sapiens
44 DTNBP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 OXTR 5021
Two-hybrid Homo sapiens
46 ABI2 10152
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 COMTD1 118881
Affinity Capture-MS Homo sapiens
48 PTPRE 5791
Proximity Label-MS Homo sapiens
49 HSPB9  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 VCP 7415
Co-fractionation Homo sapiens
51 NTRK1 4914
Affinity Capture-MS Homo sapiens
52 GPR182  
Affinity Capture-MS Homo sapiens
53 MKRN2 23609
Affinity Capture-RNA Homo sapiens
54 CCDC22 28952
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 COX7B 1349
Co-fractionation Homo sapiens
56 ACTG1 71
Affinity Capture-MS Homo sapiens
57 ACTN1 87
Co-fractionation Homo sapiens
58 VIPR2  
Affinity Capture-MS Homo sapiens
59 PSMA3 5684
Co-fractionation Homo sapiens
60 NCKAP1 10787
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 CD6 923
Affinity Capture-MS Homo sapiens
62 MGARP  
Affinity Capture-MS Homo sapiens
63 YWHAZ 7534
Affinity Capture-MS Homo sapiens
64 PACSIN3 29763
Affinity Capture-MS Homo sapiens
65 AQP3  
Affinity Capture-MS Homo sapiens
66 SMDT1  
Co-fractionation Homo sapiens
67 TSPAN8 7103
Affinity Capture-MS Homo sapiens
68 EPS8L1 54869
Affinity Capture-MS Homo sapiens
69 RPA3 6119
Proximity Label-MS Homo sapiens
70 AMZ1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 NYAP1  
Affinity Capture-Western Homo sapiens
72 GAS7  
Reconstituted Complex Homo sapiens
73 BAIAP2 10458
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 KIAA1522 57648
Affinity Capture-MS Homo sapiens
75 CBLC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 MYO16 23026
Affinity Capture-Western Homo sapiens
77 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
78 NYAP2  
Affinity Capture-Western Homo sapiens
79 GRB2 2885
Affinity Capture-MS Homo sapiens
80 PLEC 5339
Cross-Linking-MS (XL-MS) Homo sapiens
81 WWOX 51741
Affinity Capture-MS Homo sapiens
82 TCERG1 10915
Reconstituted Complex Homo sapiens
83 FXR1 8087
Two-hybrid Homo sapiens
84 PDHA1 5160
Co-fractionation Homo sapiens
85 ZNF511  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 SMAD4  
Two-hybrid Homo sapiens
87 UBXN11  
Affinity Capture-MS Homo sapiens
88 DNMBP 23268
Affinity Capture-MS Homo sapiens
89 MFSD4  
Affinity Capture-MS Homo sapiens
90 DDX58 23586
Affinity Capture-RNA Homo sapiens
91 NCK2 8440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 SPG11 80208
Affinity Capture-MS Homo sapiens
93 C10orf88  
Affinity Capture-MS Homo sapiens
94 NOC2L 26155
Cross-Linking-MS (XL-MS) Homo sapiens
95 TCF4  
Affinity Capture-MS Homo sapiens
96 FGFR1OP2  
Affinity Capture-MS Homo sapiens
97 EPHA2 1969
Proximity Label-MS Homo sapiens
98 VIPR1  
Affinity Capture-MS Homo sapiens
99 NHSL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 ILVBL 10994
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CYFIP2 is involved
PathwayEvidenceSource
Disease TAS Reactome
Disease IEA Reactome
Fcgamma receptor (FCGR) dependent phagocytosis TAS Reactome
Fcgamma receptor (FCGR) dependent phagocytosis IEA Reactome
FCGR3A-mediated phagocytosis TAS Reactome
FCGR3A-mediated phagocytosis IEA Reactome
Immune System TAS Reactome
Immune System IEA Reactome
Infectious disease TAS Reactome
Infectious disease IEA Reactome
Innate Immune System TAS Reactome
Innate Immune System IEA Reactome
Leishmania infection TAS Reactome
Leishmania infection IEA Reactome
Leishmania phagocytosis TAS Reactome
Leishmania phagocytosis IEA Reactome
Parasite infection TAS Reactome
Parasite infection IEA Reactome
Parasitic Infection Pathways TAS Reactome
Parasitic Infection Pathways IEA Reactome
RAC1 GTPase cycle TAS Reactome
Regulation of actin dynamics for phagocytic cup formation TAS Reactome
Regulation of actin dynamics for phagocytic cup formation IEA Reactome
RHO GTPase cycle TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPase Effectors IEA Reactome
RHO GTPases Activate WASPs and WAVEs TAS Reactome
RHO GTPases Activate WASPs and WAVEs IEA Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases IEA Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 IEA Reactome
Signaling by VEGF TAS Reactome
VEGFA-VEGFR2 Pathway TAS Reactome





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