Gene description for SAE1
Gene name SUMO1 activating enzyme subunit 1
Gene symbol SAE1
Other names/aliases AOS1
HSPC140
SUA1
UBLE1A
Species Homo sapiens
 Database cross references - SAE1
ExoCarta ExoCarta_10055
Entrez Gene 10055
HGNC 30660
MIM 613294
UniProt Q9UBE0  
 SAE1 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for SAE1
Molecular Function
    ubiquitin activating enzyme activity GO:0004839 TAS
    SUMO activating enzyme activity GO:0019948 IDA
    protein binding GO:0005515 IPI
    protein C-terminus binding GO:0008022 IDA
    ATP-dependent protein binding GO:0043008 IDA
    enzyme activator activity GO:0008047 TAS
    protein heterodimerization activity GO:0046982 TAS
Biological Process
    protein ubiquitination GO:0016567 TAS
    positive regulation of protein targeting to mitochondrion GO:1903955 IMP
    positive regulation of catalytic activity GO:0043085 TAS
    regulation of mitotic cell cycle GO:0007346 IBA
    post-translational protein modification GO:0043687 TAS
    protein sumoylation GO:0016925 TAS
    cellular protein metabolic process GO:0044267 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    intracellular membrane-bounded organelle GO:0043231 IDA
    cytosol GO:0005829 IBA
    SUMO activating enzyme complex GO:0031510 IDA
    nucleus GO:0005634 NAS
 Experiment description of studies that identified SAE1 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SAE1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KIAA1409  
Affinity Capture-MS Homo sapiens
2 SUMO3 6612
Affinity Capture-MS Homo sapiens
3 UBE2I 7329
Affinity Capture-MS Homo sapiens
4 UBA2 10054
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
5 FKBP4 2288
Affinity Capture-MS Homo sapiens
6 CYP1B1  
Affinity Capture-MS Homo sapiens
7 SUMO1 7341
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which SAE1 is involved
PathwayEvidenceSource
SUMO is conjugated to E1 (UBA2:SAE1) TAS Reactome
SUMO is transferred from E1 to E2 (UBE2I, UBC9) TAS Reactome





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