Gene description for UBA3
Gene name ubiquitin-like modifier activating enzyme 3
Gene symbol UBA3
Other names/aliases NAE2
UBE1C
hUBA3
Species Homo sapiens
 Database cross references - UBA3
ExoCarta ExoCarta_9039
Vesiclepedia VP_9039
Entrez Gene 9039
HGNC 12470
MIM 603172
UniProt Q8TBC4  
 UBA3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for UBA3
Molecular Function
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    NEDD8 activating enzyme activity GO:0019781 IDA
    NEDD8 activating enzyme activity GO:0019781 IDA
    NEDD8 transferase activity GO:0019788 TAS
    identical protein binding GO:0042802 IPI
    protein heterodimerization activity GO:0046982 IPI
Biological Process
    proteolysis GO:0006508 TAS
    endomitotic cell cycle GO:0007113 IEA
    protein modification process GO:0036211 TAS
    post-translational protein modification GO:0043687 TAS
    protein neddylation GO:0045116 IBA
    protein neddylation GO:0045116 IDA
    protein neddylation GO:0045116 IEA
    regulation of cell cycle GO:0051726 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified UBA3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for UBA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Co-fractionation Homo sapiens
2 UBE2F  
Biochemical Activity Homo sapiens
3 ANKRD39  
Affinity Capture-MS Homo sapiens
4 MCM3 4172
Co-fractionation Homo sapiens
5 PFN2 5217
Co-fractionation Homo sapiens
6 NSFL1C 55968
Co-fractionation Homo sapiens
7 IMPA2 3613
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 FASN 2194
Positive Genetic Homo sapiens
9 SERBP1 26135
Affinity Capture-MS Homo sapiens
10 TWF1 5756
Co-fractionation Homo sapiens
11 AHCY 191
Co-fractionation Homo sapiens
12 MAGEA3  
Affinity Capture-MS Homo sapiens
13 UFC1 51506
Co-fractionation Homo sapiens
14 UIMC1  
Affinity Capture-MS Homo sapiens
15 PRNP 5621
Affinity Capture-MS Homo sapiens
16 XPO1 7514
Co-fractionation Homo sapiens
17 RIT1 6016
Negative Genetic Homo sapiens
18 UBE2M 9040
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
19 CAMK1D 57118
Co-fractionation Homo sapiens
20 NF2 4771
Affinity Capture-MS Homo sapiens
21 NT5DC1 221294
Co-fractionation Homo sapiens
22 ACAT2 39
Co-fractionation Homo sapiens
23 CPNE2 221184
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
24 NAE1 8883
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
25 ATG7 10533
Co-fractionation Homo sapiens
26 EGFR 1956
Negative Genetic Homo sapiens
27 DCPS 28960
Co-fractionation Homo sapiens
28 TRIM55  
Two-hybrid Homo sapiens
29 PARK2  
Affinity Capture-MS Homo sapiens
30 TXNDC17 84817
Co-fractionation Homo sapiens
31 MSC  
Affinity Capture-MS Homo sapiens
32 WDR61 80349
Co-fractionation Homo sapiens
33 SUZ12  
Affinity Capture-MS Homo sapiens
34 OPTN 10133
Affinity Capture-MS Homo sapiens
35 NUDCD2 134492
Co-fractionation Homo sapiens
36 RPS6KB1 6198
Co-fractionation Homo sapiens
37 UBE2D2 7322
Reconstituted Complex Homo sapiens
38 FAM81B  
Affinity Capture-MS Homo sapiens
39 LRTM1  
Affinity Capture-MS Homo sapiens
40 KDM1A 23028
Two-hybrid Homo sapiens
41 UBA3 9039
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
42 LDLR 3949
Positive Genetic Homo sapiens
43 OGT 8473
Reconstituted Complex Homo sapiens
44 PAPSS1 9061
Co-fractionation Homo sapiens
45 SAR1B 51128
Affinity Capture-MS Homo sapiens
46 CHD3 1107
Two-hybrid Homo sapiens
47 PCYT2 5833
Co-fractionation Homo sapiens
48 NEDD8 4738
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 LIG1 3978
Co-fractionation Homo sapiens
50 DSTN 11034
Co-fractionation Homo sapiens
51 KRAS 3845
Negative Genetic Homo sapiens
52 DFFA 1676
Co-fractionation Homo sapiens
53 VCL 7414
Co-fractionation Homo sapiens
54 PRDX2 7001
Co-fractionation Homo sapiens
55 LMNA 4000
Proximity Label-MS Homo sapiens
56 RPA3 6119
Proximity Label-MS Homo sapiens
57 CAPN2 824
Co-fractionation Homo sapiens
58 DUSP23 54935
Two-hybrid Homo sapiens
59 TRIM63  
Two-hybrid Homo sapiens
60 APIP 51074
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 ZBTB44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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