Gene description for RABGEF1
Gene name RAB guanine nucleotide exchange factor (GEF) 1
Gene symbol RABGEF1
Other names/aliases RABEX5
RAP1
rabex-5
Species Homo sapiens
 Database cross references - RABGEF1
ExoCarta ExoCarta_27342
Vesiclepedia VP_27342
Entrez Gene 27342
HGNC 17676
MIM 609700
UniProt Q9UJ41  
 RABGEF1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for RABGEF1
Molecular Function
    DNA binding GO:0003677 IEA
    guanyl-nucleotide exchange factor activity GO:0005085 IBA
    guanyl-nucleotide exchange factor activity GO:0005085 IDA
    guanyl-nucleotide exchange factor activity GO:0005085 IMP
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    small GTPase binding GO:0031267 IBA
    small GTPase binding GO:0031267 IPI
    ubiquitin protein ligase activity GO:0061630 IEA
Biological Process
    negative regulation of leukocyte migration GO:0002686 IEA
    protein targeting to membrane GO:0006612 IMP
    receptor-mediated endocytosis GO:0006898 IEA
    Ras protein signal transduction GO:0007265 IEA
    protein transport GO:0015031 IEA
    negative regulation of interleukin-6 production GO:0032715 IEA
    negative regulation of mast cell cytokine production GO:0032764 IEA
    negative regulation of mast cell activation GO:0033004 IEA
    Kit signaling pathway GO:0038109 IEA
    mast cell degranulation GO:0043303 IEA
    negative regulation of mast cell degranulation GO:0043305 IEA
    negative regulation of Ras protein signal transduction GO:0046580 IEA
    negative regulation of receptor-mediated endocytosis GO:0048261 IEA
    negative regulation of inflammatory response GO:0050728 IEA
    regulation of Fc receptor mediated stimulatory signaling pathway GO:0060368 IEA
    mast cell migration GO:0097531 IEA
    dendritic transport GO:0098935 IEA
    negative regulation of Kit signaling pathway GO:1900235 IEA
Subcellular Localization
    nucleolus GO:0005730 IDA
    early endosome GO:0005769 IMP
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    endocytic vesicle GO:0030139 IBA
    dendrite GO:0030425 IEA
    early endosome membrane GO:0031901 TAS
    recycling endosome GO:0055037 IEA
    presynaptic endosome GO:0098830 IEA
 Experiment description of studies that identified RABGEF1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RABGEF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAB22A 57403
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 CRBN  
Affinity Capture-Western Homo sapiens
3 OTUD5 55593
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 NUF2  
Affinity Capture-MS Homo sapiens
5 BLOC1S6  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 CCDC36  
Two-hybrid Homo sapiens
7 ATG9A 79065
Proximity Label-MS Homo sapiens
8 RABEP2 79874
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 NRSN1  
Affinity Capture-MS Homo sapiens
10 KCTD10 83892
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CCDC132 55610
Affinity Capture-MS Homo sapiens
12 GIT2 9815
Affinity Capture-MS Homo sapiens
13 GAK 2580
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 GAPDHS 26330
Affinity Capture-MS Homo sapiens
15 CCDC183  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 HRAS 3265
Biochemical Activity Homo sapiens
17 DGCR6  
Two-hybrid Homo sapiens
18 GRIPAP1 56850
Affinity Capture-MS Homo sapiens
19 MON1A 84315
Reconstituted Complex Homo sapiens
20 UBC 7316
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
21 ROCK2 9475
Co-fractionation Homo sapiens
22 TUFT1  
Affinity Capture-MS Homo sapiens
23 ARNTL  
Affinity Capture-MS Homo sapiens
24 TRIM54  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
25 BTN3A1 11119
Affinity Capture-MS Homo sapiens
26 ANKRD29  
Two-hybrid Homo sapiens
27 EMILIN1 11117
Two-hybrid Homo sapiens
28 EXOC1 55763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 RASAL3 64926
Two-hybrid Homo sapiens
30 SESTD1 91404
Two-hybrid Homo sapiens
31 PSMD9 5715
Affinity Capture-MS Homo sapiens
32 OIP5  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
33 VTI1B 10490
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RAB21 23011
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 WASH1 100287171
Two-hybrid Homo sapiens
36 HAUS6  
Affinity Capture-MS Homo sapiens
37 GGA1 26088
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
38 RGS9BP  
Affinity Capture-MS Homo sapiens
39 L1CAM 3897
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
40 VPS53 55275
Affinity Capture-MS Homo sapiens
41 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 PKN3 29941
Affinity Capture-MS Homo sapiens
43 TMEM136  
Affinity Capture-MS Homo sapiens
44 THOC1 9984
Two-hybrid Homo sapiens
45 CPNE5 57699
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 TRIM15  
Two-hybrid Homo sapiens
47 RNF11 26994
Two-hybrid Homo sapiens
48 EEA1 8411
Affinity Capture-Western Homo sapiens
49 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 C15orf59  
Affinity Capture-MS Homo sapiens
51 MAPK1 5594
Affinity Capture-Western Homo sapiens
52 RABGEF1 27342
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
53 CLOCK  
Affinity Capture-MS Homo sapiens
54 CPNE2 221184
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 SNAP91 9892
Affinity Capture-MS Homo sapiens
56 RABEP1 9135
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 HSP90B1 7184
Co-fractionation Homo sapiens
58 TNKS1BP1 85456
Co-fractionation Homo sapiens
59 RAB4A 5867
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 DAZAP2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
61 FGG 2266
Affinity Capture-MS Homo sapiens
62 NTRK1 4914
Affinity Capture-MS Homo sapiens
63 TCHP  
Two-hybrid Homo sapiens
64 ARRDC3 57561
Two-hybrid Homo sapiens
65 UBE2D3 7323
Reconstituted Complex Homo sapiens
66 HGFAC 3083
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 UBC 444874
Co-crystal Structure Bos taurus
68 CIPC  
Two-hybrid Homo sapiens
69 TEAD4  
Two-hybrid Homo sapiens
70 YWHAH 7533
Affinity Capture-MS Homo sapiens
71 TXN2 25828
Two-hybrid Homo sapiens
72 YEATS4  
Affinity Capture-MS Homo sapiens
73 NCKAP5L  
Affinity Capture-MS Homo sapiens
74 BIRC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 FAM189B  
Affinity Capture-MS Homo sapiens
76 S100P 6286
Affinity Capture-MS Homo sapiens
77 CLINT1 9685
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 TAX1BP1 8887
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 RAB5A 5868
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
80 MON1B 22879
Reconstituted Complex Homo sapiens
81 SSX3  
Two-hybrid Homo sapiens
82 TNFRSF1A 7132
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 PHLDB3  
Affinity Capture-MS Homo sapiens
84 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 EGFR 1956
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
86 TFPT  
Affinity Capture-MS Homo sapiens
87 TMEM74  
Affinity Capture-MS Homo sapiens
88 GPRASP2  
Two-hybrid Homo sapiens
89 CCDC85A  
Affinity Capture-MS Homo sapiens
90 KCNE3  
Affinity Capture-MS Homo sapiens
91 UBR1 197131
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 PHF21A  
Affinity Capture-MS Homo sapiens
93 RABGAP1L 9910
Affinity Capture-MS Homo sapiens
94 PTPN23 25930
Proximity Label-MS Homo sapiens
95 ZC2HC1C  
Two-hybrid Homo sapiens
96 HMG20A  
Affinity Capture-MS Homo sapiens
97 UBE2D1 7321
Reconstituted Complex Homo sapiens
98 HERC1 8925
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 DZIP3  
Affinity Capture-MS Homo sapiens
100 RAD23A 5886
Two-hybrid Homo sapiens
101 FASN 2194
Negative Genetic Homo sapiens
102 TRAF2 7186
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 SLMAP 7871
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 MAX  
Affinity Capture-MS Homo sapiens
105 CCHCR1  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
106 RNF31 55072
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
107 CDC37 11140
Two-hybrid Homo sapiens
108 UBA52 7311
Two-hybrid Homo sapiens
109 DCTN2 10540
Two-hybrid Homo sapiens
110 THAP11 57215
Affinity Capture-MS Homo sapiens
111 SNAPIN 23557
Two-hybrid Homo sapiens
112 RBCK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 CCDC93 54520
Affinity Capture-MS Homo sapiens
114 ESR1  
Affinity Capture-Western Homo sapiens
115 ABI2 10152
Two-hybrid Homo sapiens
116 GOLGA5 9950
Affinity Capture-MS Homo sapiens
117 CYHR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 RAB11A 8766
Proximity Label-MS Homo sapiens
119 KXD1 79036
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
120 SPG20 23111
Two-hybrid Homo sapiens
121 SHARPIN  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 RAB5C 5878
Proximity Label-MS Homo sapiens
123 TMEM31  
Affinity Capture-MS Homo sapiens
124 PTRF 284119
Affinity Capture-MS Homo sapiens
125 CAV1 857
Affinity Capture-MS Homo sapiens
126 RCOR1  
Affinity Capture-MS Homo sapiens
127 RPRD1A  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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