Gene description for SUMO3
Gene name small ubiquitin-like modifier 3
Gene symbol SUMO3
Other names/aliases SMT3A
SMT3H1
SUMO-3
Smt3B
Species Homo sapiens
 Database cross references - SUMO3
ExoCarta ExoCarta_6612
Vesiclepedia VP_6612
Entrez Gene 6612
HGNC 11124
MIM 602231
UniProt P55854  
 SUMO3 identified in exosomes derived from the following tissue/cell type
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
 Gene ontology annotations for SUMO3
Molecular Function
    protein binding GO:0005515 IPI
    protein tag activity GO:0031386 IBA
    ubiquitin-like protein ligase binding GO:0044389 IBA
Biological Process
    protein sumoylation GO:0016925 IBA
    protein sumoylation GO:0016925 IDA
    negative regulation of DNA binding GO:0043392 IDA
    regulation of protein localization to nucleus GO:1900180 IDA
Subcellular Localization
    kinetochore GO:0000776 TAS
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IEA
    PML body GO:0016605 IDA
 Experiment description of studies that identified SUMO3 in exosomes
1
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
2
Experiment ID 274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for SUMO3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TK1 7083
Two-hybrid Homo sapiens
2 USPL1  
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
3 RNF4 6047
Affinity Capture-MS Homo sapiens
4 TTR 7276
Two-hybrid Homo sapiens
5 Rnf4  
Co-crystal Structure Mus musculus
6 UBE3A 7337
Two-hybrid Homo sapiens
7 UBC 7316
Affinity Capture-Western Homo sapiens
8 SYDE2  
Affinity Capture-MS Homo sapiens
9 ARHGDIA 396
Co-fractionation Homo sapiens
10 ZBTB33  
Two-hybrid Homo sapiens
11 CHAF1A  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
12 ISYNA1 51477
Co-fractionation Homo sapiens
13 SENP1  
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
14 FAM221A  
Two-hybrid Homo sapiens
15 TDP2 51567
Reconstituted Complex Homo sapiens
16 OTUB2  
Co-localization Homo sapiens
17 TDG 6996
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
18 STIP1 10963
Co-fractionation Homo sapiens
19 PGD 5226
Co-fractionation Homo sapiens
20 TAGLN3 29114
Co-fractionation Homo sapiens
21 SEC23A 10484
Co-fractionation Homo sapiens
22 RAD23A 5886
Co-fractionation Homo sapiens
23 DYNLT1 6993
Co-fractionation Homo sapiens
24 PFKM 5213
Co-fractionation Homo sapiens
25 ANXA7 310
Two-hybrid Homo sapiens
26 RAD23B 5887
Co-fractionation Homo sapiens
27 SENP2 59343
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
28 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
29 CDKN1A  
Two-hybrid Homo sapiens
30 SAE1 10055
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
31 MAPRE1 22919
Co-fractionation Homo sapiens
32 IRF7  
Co-localization Homo sapiens
33 MAPKAPK3 7867
Two-hybrid Homo sapiens
34 UBE2I 7329
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
35 CARM1 10498
Affinity Capture-MS Homo sapiens
36 DRAP1 10589
Co-fractionation Homo sapiens
37 RAN 5901
Affinity Capture-MS Homo sapiens
38 RNF168  
Affinity Capture-Western Homo sapiens
39 SLX4  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
40 PIAS2  
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
41 SUMO2 6613
Affinity Capture-MS Homo sapiens
42 ZNF496  
Two-hybrid Homo sapiens
43 ENO1 2023
Affinity Capture-RNA Homo sapiens
44 TAGLN2 8407
Co-fractionation Homo sapiens
45 PML 5371
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
46 YAP1 10413
Reconstituted Complex Homo sapiens
47 DAXX  
Reconstituted Complex Homo sapiens
48 SP100 6672
Reconstituted Complex Homo sapiens
49 UFD1L 7353
Co-fractionation Homo sapiens
50 XPO1 7514
Affinity Capture-MS Homo sapiens
51 ALDOB 229
Co-fractionation Homo sapiens
52 MTAP 4507
Co-fractionation Homo sapiens
53 PIAS3  
Reconstituted Complex Homo sapiens
54 ECHS1 1892
Co-fractionation Homo sapiens
55 NXF1 10482
Affinity Capture-RNA Homo sapiens
56 UBA2 10054
Affinity Capture-MS Homo sapiens
57 PIAS1 8554
Reconstituted Complex Homo sapiens
58 ZBTB39  
Two-hybrid Homo sapiens
59 ARC  
Affinity Capture-Western Homo sapiens
60 ACTN2 88
Affinity Capture-MS Homo sapiens
61 ENO2 2026
Co-fractionation Homo sapiens
62 RANBP1 5902
Affinity Capture-MS Homo sapiens
63 SENP5  
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
64 UPF2 26019
Two-hybrid Homo sapiens
65 ZCCHC12  
Two-hybrid Homo sapiens
66 Senp2  
Reconstituted Complex Rattus norvegicus
Biochemical Activity Rattus norvegicus
67 PROSC 11212
Co-fractionation Homo sapiens
68 SLC16A1 6566
Cross-Linking-MS (XL-MS) Homo sapiens
69 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
70 SMN1 6606
Two-hybrid Homo sapiens
71 USP25  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
72 TP53 7157
Affinity Capture-MS Homo sapiens
73 HOMEZ  
Two-hybrid Homo sapiens
74 INPPL1 3636
Affinity Capture-MS Homo sapiens
75 SEC23B 10483
Co-fractionation Homo sapiens
76 PFDN1 5201
Two-hybrid Homo sapiens
77 UCHL3 7347
Co-fractionation Homo sapiens
78 Crebbp  
FRET Mus musculus
79 MOV10 4343
Affinity Capture-RNA Homo sapiens
80 Senp1  
Reconstituted Complex Mus musculus
Biochemical Activity Mus musculus
81 WWTR1 25937
Reconstituted Complex Homo sapiens
82 RNF111 54778
Reconstituted Complex Homo sapiens
83 HIPK2 28996
Reconstituted Complex Homo sapiens
84 VIM 7431
Two-hybrid Homo sapiens
85 FKBP2 2286
Co-fractionation Homo sapiens
86 Nfatc2ip 18020
Reconstituted Complex Mus musculus
View the network image/svg+xml



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