Gene description for SSR3
Gene name signal sequence receptor, gamma (translocon-associated protein gamma)
Gene symbol SSR3
Other names/aliases TRAPG
Species Homo sapiens
 Database cross references - SSR3
ExoCarta ExoCarta_6747
Entrez Gene 6747
HGNC 11325
MIM 606213
UniProt Q9UNL2  
 SSR3 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for SSR3
Biological Process
    gene expression GO:0010467 TAS
    SRP-dependent cotranslational protein targeting to membrane GO:0006614 TAS
    translation GO:0006412 TAS
    cellular protein metabolic process GO:0044267 TAS
Subcellular Localization
    Sec61 translocon complex GO:0005784 IEA
    integral component of endoplasmic reticulum membrane GO:0030176 IEA
 Experiment description of studies that identified SSR3 in exosomes
1
Experiment ID 226
ISEV standards
EM
EV Biophysical techniques
GAPDH
EV Cytosolic markers
CD9|FLOT1
EV Membrane markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SSR3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C20orf24  
Affinity Capture-MS Homo sapiens
2 TCEAL1  
Affinity Capture-MS Homo sapiens
3 PRRC2A 7916
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which SSR3 is involved
PathwayEvidenceSource
SRP-dependent cotranslational protein targeting to membrane TAS Reactome





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