Gene description for Prpf8
Gene name pre-mRNA processing factor 8
Gene symbol Prpf8
Other names/aliases AU019467
D11Bwg0410e
DBF3/PRP8
Prp8
Sfprp8l
Species Mus musculus
 Database cross references - Prpf8
ExoCarta ExoCarta_192159
Vesiclepedia VP_192159
Entrez Gene 192159
UniProt Q99PV0  
 Prpf8 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Neural stem cells 25242146    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Prpf8
Molecular Function
    metallopeptidase activity GO:0008237 IEA
    U6 snRNA binding GO:0017070 IBA
    U6 snRNA binding GO:0017070 IEA
    U1 snRNA binding GO:0030619 IBA
    U2 snRNA binding GO:0030620 IBA
    U5 snRNA binding GO:0030623 IBA
    U5 snRNA binding GO:0030623 IEA
    K63-linked polyubiquitin modification-dependent protein binding GO:0070530 ISO
    K63-linked polyubiquitin modification-dependent protein binding GO:0070530 ISS
    pre-mRNA intronic binding GO:0097157 IBA
Biological Process
    spliceosomal tri-snRNP complex assembly GO:0000244 IBA
    spliceosomal tri-snRNP complex assembly GO:0000244 ISO
    spliceosomal tri-snRNP complex assembly GO:0000244 ISS
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISO
    mRNA splicing, via spliceosome GO:0000398 ISS
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to lipopolysaccharide GO:0071222 ISO
    cellular response to tumor necrosis factor GO:0071356 IEA
    cellular response to tumor necrosis factor GO:0071356 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleus GO:0005634 ISS
    U5 snRNP GO:0005682 IBA
    U5 snRNP GO:0005682 ISO
    nuclear speck GO:0016607 IEA
    small nuclear ribonucleoprotein complex GO:0030532 ISO
    U4/U6 x U5 tri-snRNP complex GO:0046540 IEA
    U4/U6 x U5 tri-snRNP complex GO:0046540 ISO
    U2-type precatalytic spliceosome GO:0071005 ISO
    U2-type precatalytic spliceosome GO:0071005 ISS
    U2-type catalytic step 1 spliceosome GO:0071006 ISO
    U2-type catalytic step 1 spliceosome GO:0071006 ISS
    U2-type catalytic step 2 spliceosome GO:0071007 ISO
    U2-type catalytic step 2 spliceosome GO:0071007 ISS
    catalytic step 2 spliceosome GO:0071013 IBA
    catalytic step 2 spliceosome GO:0071013 ISO
 Experiment description of studies that identified Prpf8 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 263
MISEV standards
✘ Biophysical techniques
✔
CD63|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25242146    
Organism Mus musculus
Experiment description Extracellular Vesicles from Neural Stem Cells Transfer IFN-? via Ifngr1 to Activate Stat1 Signaling in Target Cells
Authors "Chiara Cossetti, Nunzio Iraci, Tim R. Mercer, Tommaso Leonardi, Emanuele Alpi, Denise Drago, Clara Alfaro-Cervello, Harpreet K. Saini, Matthew P. Davis, Julia Schaeffer, Beatriz Vega, Matilde Stefanini, CongJian Zhao, Werner Muller, Jose Manuel Garcia-Verdugo, Suresh Mathivanan, Angela Bachi, Anton J. Enright, John S. Mattick, Stefano Pluchino"
Journal name Molecular Cell
Publication year 2014
Sample Neural stem cells
Sample name NPCs - Th1 treated
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.20 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Prpf8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SNRPG 6637
Affinity Capture-MS Homo sapiens
2 SNRPE 6635
Affinity Capture-MS Homo sapiens
3 PPIE 10450
Affinity Capture-MS Homo sapiens
4 ZNHIT2 741
Affinity Capture-MS Homo sapiens
5 USP39 10713
Affinity Capture-MS Homo sapiens
6 C9orf78 51759
Affinity Capture-MS Homo sapiens
7 LSM4 25804
Affinity Capture-MS Homo sapiens
8 Snrpd2 107686
Co-fractionation Mus musculus
9 PRPF3  
Affinity Capture-MS Homo sapiens
10 AAR2 25980
Affinity Capture-MS Homo sapiens
11 AQR 9716
Affinity Capture-MS Homo sapiens
12 SYF2  
Affinity Capture-MS Homo sapiens
13 SNRPD2 6633
Affinity Capture-MS Homo sapiens
14 Thoc2  
Co-fractionation Mus musculus
15 Plrg1  
Co-fractionation Mus musculus
16 RUVBL1 8607
Affinity Capture-MS Homo sapiens
17 CRNKL1 51340
Affinity Capture-MS Homo sapiens
18 Srrm2  
Co-fractionation Mus musculus
19 Snw1  
Co-fractionation Mus musculus
20 DHX35  
Affinity Capture-MS Homo sapiens
21 Hnrnpk 15387
Co-fractionation Mus musculus
22 TFIP11  
Affinity Capture-MS Homo sapiens
23 Prpf4  
Co-fractionation Mus musculus
24 RSRC1  
Affinity Capture-MS Homo sapiens
25 SNRPD3 6634
Affinity Capture-MS Homo sapiens
26 PRPF6 24148
Affinity Capture-MS Homo sapiens
27 PRPF8 10594
Affinity Capture-MS Homo sapiens
28 Frg1  
Co-fractionation Mus musculus
29 Prpf19 28000
Co-fractionation Mus musculus
30 Snrnp35  
Co-fractionation Mus musculus
31 PPIL1 51645
Affinity Capture-MS Homo sapiens
32 Ddx17 67040
Co-fractionation Mus musculus
33 Sf3b3 101943
Co-fractionation Mus musculus
34 PPIH 10465
Affinity Capture-MS Homo sapiens
35 SNRNP200 23020
Affinity Capture-MS Homo sapiens
36 SNRPB 6628
Affinity Capture-MS Homo sapiens
37 SNRPD1 6632
Affinity Capture-MS Homo sapiens
38 Spen  
Co-fractionation Mus musculus
39 Tfip11  
Co-fractionation Mus musculus
40 Ppwd1  
Co-fractionation Mus musculus
41 Hnrnpm 76936
Co-fractionation Mus musculus
42 Hnrnpf 98758
Co-fractionation Mus musculus
43 Snrnp48 67797
Co-fractionation Mus musculus
44 SNRNP27  
Affinity Capture-MS Homo sapiens
45 Skiv2l2  
Co-fractionation Mus musculus
46 Eif4a3 192170
Co-fractionation Mus musculus
47 PRPF31 26121
Affinity Capture-MS Homo sapiens
48 Paxbp1  
Co-fractionation Mus musculus
49 Tcf3  
Affinity Capture-MS Mus musculus
50 CCDC12  
Affinity Capture-MS Homo sapiens
51 Ddx5 13207
Co-fractionation Mus musculus
52 LSM8 51691
Affinity Capture-MS Homo sapiens
53 Prpf4b 19134
Co-fractionation Mus musculus
54 ECD  
Affinity Capture-MS Homo sapiens
55 Sart3  
Co-fractionation Mus musculus
56 CD2BP2 10421
Affinity Capture-MS Homo sapiens
57 Alyref  
Co-fractionation Mus musculus
58 PLRG1 5356
Affinity Capture-MS Homo sapiens
59 Hnrnpu 51810
Co-fractionation Mus musculus
60 XAB2 56949
Affinity Capture-MS Homo sapiens
61 TSSC4  
Affinity Capture-MS Homo sapiens
62 NCDN 23154
Affinity Capture-MS Homo sapiens
63 SART1 9092
Affinity Capture-MS Homo sapiens
64 EFTUD2 9343
Affinity Capture-MS Homo sapiens
65 PRPF19 27339
Affinity Capture-MS Homo sapiens
66 Hnrnpa3 229279
Co-fractionation Mus musculus
67 2310036O22Rik  
Co-fractionation Mus musculus
68 Syncrip 56403
Co-fractionation Mus musculus
69 NHP2L1 4809
Affinity Capture-MS Homo sapiens
70 Ddx41  
Co-fractionation Mus musculus
71 Eed  
Affinity Capture-MS Mus musculus
72 Zfp830 66983
Co-fractionation Mus musculus
73 Prpf31  
Co-fractionation Mus musculus
74 Ppil3  
Co-fractionation Mus musculus
75 Fancd2  
Affinity Capture-MS Mus musculus
76 Sf3a3 75062
Co-fractionation Mus musculus
77 Prpf18  
Co-fractionation Mus musculus
78 PAXBP1  
Affinity Capture-MS Homo sapiens
79 DHX15 1665
Affinity Capture-MS Homo sapiens
80 EAPP  
Affinity Capture-MS Homo sapiens
81 Prpf40a  
Co-fractionation Mus musculus
82 BCAS2 10286
Affinity Capture-MS Homo sapiens
83 Cdc5l 71702
Co-fractionation Mus musculus
84 TTC27 55622
Affinity Capture-MS Homo sapiens
85 RUVBL2 10856
Affinity Capture-MS Homo sapiens
86 Rbm8a  
Co-fractionation Mus musculus
87 Atxn1  
Affinity Capture-MS Mus musculus
88 Rbm39 170791
Co-fractionation Mus musculus
89 Cwc22  
Co-fractionation Mus musculus
90 Chaf1a  
Affinity Capture-MS Mus musculus
91 DDX23 9416
Affinity Capture-MS Homo sapiens
92 CWC15  
Affinity Capture-MS Homo sapiens
93 Prpf3 70767
Co-fractionation Mus musculus
94 Mettl21e  
Affinity Capture-MS Mus musculus
95 Hnrnph1 59013
Co-fractionation Mus musculus
96 SNRNP40 9410
Affinity Capture-MS Homo sapiens
97 DHX38 9785
Affinity Capture-MS Homo sapiens
98 Gpatch1  
Co-fractionation Mus musculus
99 Dhx15 13204
Co-fractionation Mus musculus
100 PRPF4 9128
Affinity Capture-MS Homo sapiens
101 SNW1 22938
Affinity Capture-MS Homo sapiens
102 Cwc27  
Co-fractionation Mus musculus
103 Cactin  
Co-fractionation Mus musculus
104 Chka  
Co-fractionation Mus musculus
105 Hnrnpa2b1 53379
Co-fractionation Mus musculus
106 CDC5L 988
Affinity Capture-MS Homo sapiens
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