ExoCarta: Gene summary

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Gene description for METTL1
Gene name	methyltransferase like 1
Gene symbol	METTL1
Other names/aliases	C12orf1 TRM8 TRMT8 YDL201w
Species	Homo sapiens
Database cross references - METTL1
ExoCarta	ExoCarta_4234
Vesiclepedia	VP_4234
Entrez Gene	4234
HGNC	7030
MIM	604466
UniProt	Q9UBP6
METTL1 identified in sEVs derived from the following tissue/cell type
Chondrocytes	35931686
Chondrocytes	35931686

Gene ontology annotations for METTL1

Molecular Function
	tRNA binding	GO:0000049	IEA
	protein binding	GO:0005515	IPI
	tRNA (guanine(46)-N7)-methyltransferase activity	GO:0008176	IBA
	tRNA (guanine(46)-N7)-methyltransferase activity	GO:0008176	IDA
	internal mRNA (guanine-N7-)-methyltransferase activity	GO:0160090	IDA
Biological Process
	tRNA modification	GO:0006400	IDA
	tRNA methylation	GO:0030488	IBA
	cellular response to stress	GO:0033554	IDA
	RNA (guanine-N7)-methylation	GO:0036265	IBA
	RNA (guanine-N7)-methylation	GO:0036265	IDA
	tRNA stabilization	GO:0036416	IDA
	tRNA (guanine-N7)-methylation	GO:0106004	IDA
Subcellular Localization
	nucleus	GO:0005634	IDA
	nucleoplasm	GO:0005654	IDA
	nucleolus	GO:0005730	IDA
	cytosol	GO:0005829	IDA
	tRNA methyltransferase complex	GO:0043527	IBA
	tRNA (m7G46) methyltransferase complex	GO:0106143	IDA

Experiment description of studies that identified METTL1 in sEVs

Experiment ID

497

MISEV standards
✘	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

"Varela-EirÃn M, Carpintero-FernÃ¡ndez P, GuitiÃ¡n-CaamaÃ±o A, Varela-VÃ¡zquez A, GarcÃa-Yuste A, SÃ¡nchez-Temprano A, Bravo-LÃ³pez SB, YaÃ±ez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, MayÃ¡n MD."

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

498

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|RAB5A\|SDCBP\|TFRC\|TSG101\|UCHL1	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

T/C-28a2

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Protein-protein interactions for METTL1

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

TSNAX

7257

Co-fractionation

Homo sapiens

ACTR2

10097

Co-fractionation

Homo sapiens

RPSAP58

388524

Affinity Capture-MS

Homo sapiens

ZWINT

Affinity Capture-MS

Homo sapiens

METTL18

Affinity Capture-MS

Homo sapiens

UBE3A

7337

Co-fractionation

Homo sapiens

KIAA0101

Co-fractionation

Homo sapiens

NPLOC4

55666

Co-fractionation

Homo sapiens

SIRT6

Affinity Capture-MS

Homo sapiens

WDR4

10785

Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-Western		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NIF3L1

60491

Co-fractionation

Homo sapiens

TP53BP1

7158

Affinity Capture-MS

Homo sapiens

ARPC1B

10095

Co-fractionation

Homo sapiens

CTPS2

56474

Co-fractionation

Homo sapiens

ARPC2

10109

Co-fractionation

Homo sapiens

GTF2I

2969

Co-fractionation

Homo sapiens

RPA2

6118

Proximity Label-MS

Homo sapiens

DR1

Co-fractionation

Homo sapiens

IPO11

51194

Co-fractionation

Homo sapiens

PRDX1

5052

Co-fractionation

Homo sapiens

DNAAF5

54919

Co-fractionation

Homo sapiens

TPD52L2

7165

Co-fractionation		Homo sapiens
Co-fractionation		Homo sapiens

C15orf40

Co-fractionation

Homo sapiens

SSB

6741

Affinity Capture-MS

Homo sapiens

PCMT1

5110

Cross-Linking-MS (XL-MS)

Homo sapiens

PRKDC

5591

Co-fractionation

Homo sapiens

APEX1

328

Co-fractionation

Homo sapiens

TRMT61A

115708

Co-fractionation

Homo sapiens

PDLIM5

10611

Co-fractionation

Homo sapiens

KIF1BP

26128

Co-fractionation

Homo sapiens

RPA3

6119

Proximity Label-MS

Homo sapiens

NUCB1

4924

Co-fractionation

Homo sapiens

ACTR3

10096

Co-fractionation

Homo sapiens

DIAPH2

Co-fractionation

Homo sapiens

NHP2L1

4809

Co-fractionation

Homo sapiens

ARPC4

10093

Co-fractionation

Homo sapiens

TP53RK

112858

Co-fractionation

Homo sapiens

PPA2

27068

Co-fractionation

Homo sapiens

TPRKB

51002

Co-fractionation

Homo sapiens

ARPC5

10092

Co-fractionation

Homo sapiens

AKT1

207

Biochemical Activity

Homo sapiens

AARS

Co-fractionation

Homo sapiens

PRDX2

7001

Co-fractionation

Homo sapiens

TOM1L1

10040

Co-fractionation

Homo sapiens

CACYBP

27101

Co-fractionation

Homo sapiens

View the network

Pathways in which METTL1 is involved

Pathway	Evidence	Source
Metabolism of RNA	IEA	Reactome
tRNA modification in the nucleus and cytosol	IEA	Reactome
tRNA processing	IEA	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here