Gene description for MAP2K4
Gene name mitogen-activated protein kinase kinase 4
Gene symbol MAP2K4
Other names/aliases JNKK
JNKK1
MAPKK4
MEK4
MKK4
PRKMK4
SAPKK-1
SAPKK1
SEK1
SERK1
SKK1
Species Homo sapiens
 Database cross references - MAP2K4
ExoCarta ExoCarta_6416
Vesiclepedia VP_6416
Entrez Gene 6416
HGNC 6844
MIM 601335
UniProt P45985  
 MAP2K4 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for MAP2K4
Molecular Function
    protein kinase activity GO:0004672 TAS
    protein serine/threonine kinase activity GO:0004674 IEA
    MAP kinase kinase activity GO:0004708 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    JUN kinase kinase activity GO:0008545 TAS
    histone H3Y41 kinase activity GO:0035401 IEA
    molecular adaptor activity GO:0060090 EXP
    protein serine kinase activity GO:0106310 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    MAPK cascade GO:0000165 IBA
    chromatin remodeling GO:0006338 IEA
    signal transduction GO:0007165 TAS
    JNK cascade GO:0007254 TAS
    response to wounding GO:0009611 IEA
    smooth muscle cell apoptotic process GO:0034390 IEA
    intrinsic apoptotic signaling pathway in response to hydrogen peroxide GO:0036481 IEA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    positive regulation of neuron apoptotic process GO:0043525 IEA
    positive regulation of DNA replication GO:0045740 IEA
    positive regulation of JNK cascade GO:0046330 IEA
    cell growth involved in cardiac muscle cell development GO:0061049 IEA
    cellular response to mechanical stimulus GO:0071260 IEP
    cellular response to sorbitol GO:0072709 IEA
    cellular senescence GO:0090398 TAS
    negative regulation of motor neuron apoptotic process GO:2000672 IEA
Subcellular Localization
    nucleus GO:0005634 IEA
    cytosol GO:0005829 TAS
    axon GO:0030424 IEA
    dendrite cytoplasm GO:0032839 IEA
    perikaryon GO:0043204 IEA
 Experiment description of studies that identified MAP2K4 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAP2K4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAP2K7 5609
Phenotypic Enhancement Homo sapiens
Affinity Capture-Western Homo sapiens
2 KTI12 112970
Co-fractionation Homo sapiens
3 SH3RF3  
Affinity Capture-Western Homo sapiens
4 ARRB2 409
Affinity Capture-Western Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 MAP2K6 5608
Biochemical Activity Homo sapiens
7 MAP3K8  
Biochemical Activity Homo sapiens
8 TNFAIP8L1  
Affinity Capture-Western Homo sapiens
9 NBR1 4077
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 MAP2K4 6416
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
11 MAP3K12  
Biochemical Activity Homo sapiens
12 MAP4K2  
Reconstituted Complex Homo sapiens
13 ZDHHC17 23390
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
14 MAPK8IP3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
15 MAP3K2 10746
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
16 UBC 7316
Reconstituted Complex Homo sapiens
17 EGFR 1956
Affinity Capture-MS Homo sapiens
18 MAP3K1 4214
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
19 NPHS1 4868
Phenotypic Suppression Homo sapiens
20 MAP3K7  
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
21 VASP 7408
Co-fractionation Homo sapiens
22 APP 351
Reconstituted Complex Homo sapiens
23 MAPK10 5602
Biochemical Activity Homo sapiens
24 APEX1 328
Affinity Capture-RNA Homo sapiens
25 JUN 3725
Biochemical Activity Homo sapiens
26 MAP3K5 4217
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
27 RPA3 6119
Proximity Label-MS Homo sapiens
28 LRRK2 120892
Biochemical Activity Homo sapiens
29 MAPK9 5601
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
30 MAP3K11 4296
Biochemical Activity Homo sapiens
31 MAP3K4 4216
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
32 SPAG9 9043
Affinity Capture-Western Homo sapiens
33 FLNC 2318
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
34 FLNB 2317
Affinity Capture-Western Homo sapiens
35 PML 5371
Co-localization Homo sapiens
36 Flna 192176
Two-hybrid Mus musculus
37 BLNK  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
38 MAPK14 1432
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
39 AKT1 207
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
40 MAP3K3 4215
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
41 MAPK8 5599
Phenotypic Enhancement Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
42 ITCH 83737
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
43 MAP3K10  
Biochemical Activity Homo sapiens
44 CDC5L 988
Affinity Capture-MS Homo sapiens
45 RBBP8  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MAP2K4 is involved
PathwayEvidenceSource
Bacterial Infection Pathways TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stimuli IEA Reactome
Cellular responses to stress TAS Reactome
Cellular responses to stress IEA Reactome
Cellular Senescence TAS Reactome
Cellular Senescence IEA Reactome
Cytokine Signaling in Immune system IEA Reactome
Cytokine Signaling in Immune system TAS Reactome
Disease TAS Reactome
Fc epsilon receptor (FCERI) signaling TAS Reactome
FCERI mediated MAPK activation TAS Reactome
Immune System IEA Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Innate Immune System IEA Reactome
Innate Immune System TAS Reactome
Interleukin-1 family signaling IEA Reactome
Interleukin-1 signaling IEA Reactome
Interleukin-17 signaling IEA Reactome
Interleukin-17 signaling TAS Reactome
JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 TAS Reactome
MAP kinase activation IEA Reactome
MAP kinase activation TAS Reactome
MAP3K8 (TPL2)-dependent MAPK1/3 activation IEA Reactome
MyD88 cascade initiated on plasma membrane IEA Reactome
MyD88 cascade initiated on plasma membrane TAS Reactome
MyD88 dependent cascade initiated on endosome IEA Reactome
MyD88 dependent cascade initiated on endosome TAS Reactome
MyD88-independent TLR4 cascade IEA Reactome
MyD88-independent TLR4 cascade TAS Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane IEA Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane TAS Reactome
Oxidative Stress Induced Senescence TAS Reactome
Oxidative Stress Induced Senescence IEA Reactome
Signaling by Interleukins IEA Reactome
Signaling by Interleukins TAS Reactome
Toll Like Receptor 10 (TLR10) Cascade IEA Reactome
Toll Like Receptor 10 (TLR10) Cascade TAS Reactome
Toll Like Receptor 2 (TLR2) Cascade IEA Reactome
Toll Like Receptor 2 (TLR2) Cascade TAS Reactome
Toll Like Receptor 3 (TLR3) Cascade IEA Reactome
Toll Like Receptor 3 (TLR3) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade IEA Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll Like Receptor 5 (TLR5) Cascade IEA Reactome
Toll Like Receptor 5 (TLR5) Cascade TAS Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade IEA Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade TAS Reactome
Toll Like Receptor 9 (TLR9) Cascade IEA Reactome
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome
Toll Like Receptor TLR1:TLR2 Cascade IEA Reactome
Toll Like Receptor TLR1:TLR2 Cascade TAS Reactome
Toll Like Receptor TLR6:TLR2 Cascade IEA Reactome
Toll Like Receptor TLR6:TLR2 Cascade TAS Reactome
Toll-like Receptor Cascades IEA Reactome
Toll-like Receptor Cascades TAS Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation IEA Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation TAS Reactome
TRIF (TICAM1)-mediated TLR4 signaling IEA Reactome
TRIF (TICAM1)-mediated TLR4 signaling TAS Reactome
Uptake and actions of bacterial toxins TAS Reactome
Uptake and function of anthrax toxins TAS Reactome





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