Gene description for FAM175B
Gene name family with sequence similarity 175, member B
Gene symbol FAM175B
Other names/aliases ABRO1
KIAA0157
Species Homo sapiens
 Database cross references - FAM175B
ExoCarta ExoCarta_23172
Vesiclepedia VP_23172
Entrez Gene 23172
HGNC 28975
MIM 611144
UniProt Q15018  
 FAM175B identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for FAM175B
Molecular Function
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IBA
    microtubule binding GO:0008017 IDA
    polyubiquitin modification-dependent protein binding GO:0031593 IBA
    polyubiquitin modification-dependent protein binding GO:0031593 IDA
Biological Process
    mitotic cell cycle GO:0000278 IMP
    response to ischemia GO:0002931 IMP
    chromosome segregation GO:0007059 IMP
    attachment of spindle microtubules to kinetochore GO:0008608 IBA
    attachment of spindle microtubules to kinetochore GO:0008608 IMP
    cell division GO:0051301 IEA
    protein K63-linked deubiquitination GO:0070536 IMP
    mitotic spindle assembly GO:0090307 IBA
    mitotic spindle assembly GO:0090307 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    midbody GO:0030496 IDA
    spindle pole centrosome GO:0031616 IDA
    microtubule minus-end GO:0036449 IDA
    BRISC complex GO:0070552 IDA
 Experiment description of studies that identified FAM175B in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for FAM175B
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POC1B 282809
Affinity Capture-MS Homo sapiens
2 PNOC  
Affinity Capture-MS Homo sapiens
3 FAM189B  
Affinity Capture-MS Homo sapiens
4 SNX27 81609
Affinity Capture-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 DPP9 91039
Affinity Capture-MS Homo sapiens
7 USP7 7874
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
8 GRB2 2885
Affinity Capture-MS Homo sapiens
9 DEF6 50619
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 UIMC1  
Affinity Capture-MS Homo sapiens
11 TP53 7157
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
12 GTPBP1 9567
Affinity Capture-MS Homo sapiens
13 WWP1 11059
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
14 XPO1 7514
Affinity Capture-MS Homo sapiens
15 FAM168A  
Affinity Capture-MS Homo sapiens
16 TNS4 84951
Affinity Capture-MS Homo sapiens
17 Tnks  
Affinity Capture-MS Mus musculus
18 BABAM1 29086
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
19 FAM19A4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 HNRNPA2B1 3181
Co-fractionation Homo sapiens
21 TNS1 7145
Affinity Capture-MS Homo sapiens
22 BSPRY 54836
Affinity Capture-MS Homo sapiens
23 SPINK14  
Affinity Capture-MS Homo sapiens
24 NUMA1 4926
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
25 B4GALNT3  
Affinity Capture-MS Homo sapiens
26 UBC 7316
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
27 ARRDC1 92714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
29 IGHG2 3501
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 THAP11 57215
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 LAMC1 3915
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 LOXL4 84171
Affinity Capture-MS Homo sapiens
33 DNAJC25 548645
Affinity Capture-MS Homo sapiens
34 SHMT2 6472
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
35 SPAG9 9043
Co-fractionation Homo sapiens
36 DNAJB3 414061
Proximity Label-MS Homo sapiens
37 ANKRD28 23243
Proximity Label-MS Homo sapiens
38 ZNF653  
Affinity Capture-MS Homo sapiens
39 TXNDC9 10190
Co-fractionation Homo sapiens
40 AUP1 550
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 DIABLO 56616
Proximity Label-MS Homo sapiens
42 WWP2 11060
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 BIRC6 57448
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 TUBB2B 347733
Affinity Capture-MS Homo sapiens
45 C2orf73  
Affinity Capture-MS Homo sapiens
46 TSC22D4 81628
Affinity Capture-MS Homo sapiens
47 TRAF7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 RPA3 6119
Proximity Label-MS Homo sapiens
49 NLRP3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
50 IGHG1 3500
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 OGT 8473
Reconstituted Complex Homo sapiens
52 C9orf85  
Affinity Capture-MS Homo sapiens
53 THAP5  
Two-hybrid Homo sapiens
54 TNS2 23371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 STK11 6794
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 PSMC5 5705
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 BRCC3 79184
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 KLHL10  
Affinity Capture-MS Homo sapiens
59 SUV39H1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 XKRX  
Affinity Capture-MS Homo sapiens
61 C9orf72  
Affinity Capture-MS Homo sapiens
62 FERMT1 55612
Affinity Capture-MS Homo sapiens
63 SMURF2 64750
Two-hybrid Homo sapiens
64 ODF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 CYTIP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 NUP62 23636
Affinity Capture-MS Homo sapiens
67 BRE 9577
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
68 ARHGEF40 55701
Affinity Capture-MS Homo sapiens
69 DNAJC6  
Proximity Label-MS Homo sapiens
70 HUWE1 10075
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which FAM175B is involved
PathwayEvidenceSource
Deubiquitination TAS Reactome
Metabolism of proteins TAS Reactome
Metalloprotease DUBs TAS Reactome
Post-translational protein modification TAS Reactome





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