Gene description for PPEF2
Gene name protein phosphatase, EF-hand calcium binding domain 2
Gene symbol PPEF2
Other names/aliases PPP7CB
Species Homo sapiens
 Database cross references - PPEF2
ExoCarta ExoCarta_5470
Vesiclepedia VP_5470
Entrez Gene 5470
HGNC 9244
MIM 602256
UniProt O14830  
 PPEF2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for PPEF2
Molecular Function
    protein serine/threonine phosphatase activity GO:0004722 IBA
    iron ion binding GO:0005506 IEA
    calcium ion binding GO:0005509 IEA
    myosin phosphatase activity GO:0017018 IEA
    manganese ion binding GO:0030145 IEA
    protein serine/threonine kinase inhibitor activity GO:0030291 IDA
    Hsp70 protein binding GO:0030544 IDA
    mitogen-activated protein kinase kinase kinase binding GO:0031435 IPI
    calmodulin-dependent protein phosphatase activity GO:0033192 IEA
    Hsp90 protein binding GO:0051879 IBA
    Hsp90 protein binding GO:0051879 IDA
    histone H2AXS140 phosphatase activity GO:0140791 IEA
    RNA polymerase II CTD heptapeptide repeat Y1 phosphatase activity GO:0180004 IEA
    RNA polymerase II CTD heptapeptide repeat T4 phosphatase activity GO:0180005 IEA
    RNA polymerase II CTD heptapeptide repeat S2 phosphatase activity GO:0180006 IEA
    RNA polymerase II CTD heptapeptide repeat S5 phosphatase activity GO:0180007 IEA
    RNA polymerase II CTD heptapeptide repeat S7 phosphatase activity GO:0180008 IEA
    MAP kinase serine/threonine phosphatase activity GO:1990439 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    regulation of transcription by RNA polymerase II GO:0006357 IEA
    protein dephosphorylation GO:0006470 TAS
    visual perception GO:0007601 IEA
    negative regulation of MAPK cascade GO:0043409 IBA
    negative regulation of MAPK cascade GO:0043409 IDA
    detection of stimulus involved in sensory perception GO:0050906 IEA
    cellular response to hydrogen peroxide GO:0070301 IDA
    negative regulation of apoptotic signaling pathway GO:2001234 IDA
Subcellular Localization
    photoreceptor outer segment GO:0001750 IEA
    photoreceptor inner segment GO:0001917 IEA
    nucleus GO:0005634 IBA
    cytosol GO:0005829 IBA
 Experiment description of studies that identified PPEF2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PPEF2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SUGT1 10910
Proximity Label-MS Homo sapiens
2 HAX1  
Affinity Capture-MS Homo sapiens
3 CALM2 805
Affinity Capture-MS Homo sapiens
4 KRT8 3856
Affinity Capture-MS Homo sapiens
5 WDR92 116143
Proximity Label-MS Homo sapiens
6 NUDCD3 23386
Proximity Label-MS Homo sapiens
7 FKBP4 2288
Proximity Label-MS Homo sapiens
8 CALM1 801
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
9 KIAA1524 57650
Proximity Label-MS Homo sapiens
10 ATP2A2 488
Affinity Capture-MS Homo sapiens
11 RCN1 5954
Affinity Capture-MS Homo sapiens
12 AKAP8L  
Affinity Capture-MS Homo sapiens
13 TUBB6 84617
Affinity Capture-MS Homo sapiens
14 UNC119 9094
Affinity Capture-MS Homo sapiens
15 URI1  
Proximity Label-MS Homo sapiens
16 RPS7 6201
Proximity Label-MS Homo sapiens
17 SPAG9 9043
Affinity Capture-MS Homo sapiens
18 RPAP3 79657
Proximity Label-MS Homo sapiens
19 PIH1D1  
Proximity Label-MS Homo sapiens
20 RCN2 5955
Affinity Capture-MS Homo sapiens
21 YWHAG 7532
Affinity Capture-MS Homo sapiens
22 SDF4 51150
Affinity Capture-MS Homo sapiens
23 STIP1 10963
Proximity Label-MS Homo sapiens
24 CACYBP 27101
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which PPEF2 is involved
No pathways found





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