Gene description for DEFA1
Gene name defensin, alpha 1
Gene symbol DEFA1
Other names/aliases DEF1
DEFA2
HNP-1
HP-1
HP1
MRS
Species Homo sapiens
 Database cross references - DEFA1
ExoCarta ExoCarta_1667
Entrez Gene 1667
HGNC 2761
MIM 125220
UniProt P59665  
 DEFA1 identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells Unpublished / Not applicable
Saliva 19199708    
Urine 15326289    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for DEFA1
Biological Process
    killing of cells of other organism GO:0031640 IEA
    defense response to virus GO:0051607 IEA
    defense response to fungus GO:0050832 IEA
    defense response to Gram-positive bacterium GO:0050830 IDA
    immune response GO:0006955 TAS
    innate immune response GO:0045087 TAS
    intracellular estrogen receptor signaling pathway GO:0030520 IDA
    chemotaxis GO:0006935 TAS
    antibacterial humoral response GO:0019731 IDA
    innate immune response in mucosa GO:0002227 IDA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular matrix GO:0031012 IDA
    azurophil granule lumen GO:0035578 TAS
    Golgi lumen GO:0005796 TAS
    extracellular exosome GO:0070062 IDA
    extracellular space GO:0005615 IDA
 Experiment description of studies that identified DEFA1 in exosomes
1
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
2
Experiment ID 66
ISEV standards
✔
IEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
3
Experiment ID 13
ISEV standards
✔
IEM
EV Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
✔
CD9|AQP2|AQP1
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
4
Experiment ID 196
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 197
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DEFA1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SERPINF2 5345
Invitro Homo sapiens
2 C1QB 713
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which DEFA1 is involved
PathwayEvidenceSource
Alpha-defensins TAS Reactome
Defensins TAS Reactome





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