Gene description for PFKFB3
Gene name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Gene symbol PFKFB3
Other names/aliases IPFK2
PFK2
Species Homo sapiens
 Database cross references - PFKFB3
ExoCarta ExoCarta_5209
Vesiclepedia VP_5209
Entrez Gene 5209
HGNC 8874
MIM 605319
UniProt Q16875  
 PFKFB3 identified in sEVs derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Mesenchymal stem cells Unpublished / Not applicable
 Gene ontology annotations for PFKFB3
Molecular Function
    6-phosphofructo-2-kinase activity GO:0003873 IBA
    6-phosphofructo-2-kinase activity GO:0003873 IDA
    6-phosphofructo-2-kinase activity GO:0003873 NAS
    fructose-2,6-bisphosphate 2-phosphatase activity GO:0004331 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    fructose metabolic process GO:0006000 IEA
    fructose 2,6-bisphosphate metabolic process GO:0006003 IBA
    glycolytic process GO:0006096 IEA
    apoptotic process GO:0006915 IEA
    glial cell differentiation GO:0010001 IEA
    carbohydrate phosphorylation GO:0046835 IEA
    motor behavior GO:0061744 IEA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PFKFB3 in sEVs
1
Experiment ID 236
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
Western blotting
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
 Protein-protein interactions for PFKFB3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 PFKFB1  
Two-hybrid Homo sapiens
3 PFKFB4 5210
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 PFKFB2 5208
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
6 ARRB2 409
Affinity Capture-MS Homo sapiens
7 UBE2H 7328
Affinity Capture-MS Homo sapiens
8 DCAF7 10238
Affinity Capture-MS Homo sapiens
9 PES1 23481
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 YWHAB 7529
Affinity Capture-MS Homo sapiens
11 CTNNB1 1499
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 KIAA1429 25962
Affinity Capture-MS Homo sapiens
13 YWHAZ 7534
Affinity Capture-MS Homo sapiens
14 GCK  
PCA Homo sapiens
15 EGFR 1956
Affinity Capture-MS Homo sapiens
16 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
17 TRIM11  
Affinity Capture-MS Homo sapiens
18 CLUH 23277
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 EIF4A3 9775
Affinity Capture-MS Homo sapiens
20 SNX27 81609
Affinity Capture-MS Homo sapiens
21 ESR1  
Affinity Capture-MS Homo sapiens
22 UFL1 23376
Affinity Capture-MS Homo sapiens
23 ASB12  
Affinity Capture-MS Homo sapiens
24 HIST1H3E 8353
Affinity Capture-MS Homo sapiens
25 OTUD4  
Reconstituted Complex Homo sapiens
26 ARRB1 408
Affinity Capture-MS Homo sapiens
27 YWHAQ 10971
Affinity Capture-MS Homo sapiens
28 LRRTM1 347730
Affinity Capture-MS Homo sapiens
29 TXNIP 10628
Affinity Capture-MS Homo sapiens
30 YWHAG 7532
Affinity Capture-MS Homo sapiens
31 MAGOH 4116
Affinity Capture-MS Homo sapiens
32 CUL2 8453
Affinity Capture-MS Homo sapiens
33 PRMT1 3276
Affinity Capture-MS Homo sapiens
34 CYLD  
Affinity Capture-MS Homo sapiens
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