Experiment description of studies that identified Ywhab in exosomes |
1 |
Experiment ID |
73 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
18494037
|
Organism |
Mus musculus |
Experiment description |
Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes. |
Authors |
Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ |
Journal name |
ELEC
|
Publication year |
2008 |
Sample |
Embryonic fibroblasts |
Sample name |
NIH3T3 |
Isolation/purification methods |
Differential centrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LCQ DECA] Western blotting |
|
|
2 |
Experiment ID |
15 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
17486113
|
Organism |
Mus musculus Homo sapiens |
Experiment description |
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. |
Authors |
Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO |
Journal name |
NCB
|
Publication year |
2007 |
Sample |
Mast cells |
Sample name |
MC9 Bone marrow-derived mast cells HMC-1 |
Isolation/purification methods |
Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.11-1.21 g/mL
|
Molecules identified in the study |
Protein mRNA miRNA |
Methods used in the study |
Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array |
|
|
3 |
Experiment ID |
15 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD63
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
mrna
|
Identification method |
Microarray
|
PubMed ID |
17486113
|
Organism |
Mus musculus Homo sapiens |
Experiment description |
Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. |
Authors |
Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO |
Journal name |
NCB
|
Publication year |
2007 |
Sample |
Mast cells |
Sample name |
MC9 Bone marrow-derived mast cells HMC-1 |
Isolation/purification methods |
Filtration Ultracentrifugation Sucrose density gradient |
Flotation density |
1.11-1.21 g/mL
|
Molecules identified in the study |
Protein mRNA miRNA |
Methods used in the study |
Mass spectrometry [MALDI TOF] Western blotting Microarray miRCURY LNA Array |
|
|
4 |
Experiment ID |
14 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
RAB7|RAB11
|
EV Cytosolic markers |
✔
CD9|CD63|LAMP1|LAMP2
|
EV Membrane markers |
✔
DNM
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
16081791
|
Organism |
Mus musculus |
Experiment description |
Proteomic analysis of microglia-derived exosomes: metabolic role of the aminopeptidase CD13 in neuropeptide catabolism. |
Authors |
Potolicchio I, Carven GJ, Xu X, Stipp C, Riese RJ, Stern LJ, Santambrogio L |
Journal name |
JIMMU
|
Publication year |
2005 |
Sample |
Microglia |
Sample name |
N9 |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.15 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [LCQ DECA XP] Western blotting |
|
|
5 |
Experiment ID |
33 |
ISEV standards |
✔
EM|IEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
21136642
|
Organism |
Mus musculus |
Experiment description |
Oligodendrocytes secrete exosomes containing major myelin and stress-protective proteins: Trophic support for axons? |
Authors |
Kramer-Albers EM, Bretz N, Tenzer S, Winterstein C, Mobius W, Berger H, Nave KA, Schild H, Trotter J |
Journal name |
PROTEOMICS_CL
|
Publication year |
2007 |
Sample |
Oligodendrocytes |
Sample name |
Oligodendrocytes Oli-neu |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
1.10-1.14 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] Western blotting Immunoelectron Microscopy |
|
|
6 |
Experiment ID |
188 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
GAPDH|UCHL1|HSP90
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
19351151
|
Organism |
Mus musculus |
Experiment description |
Characterization of vesicles secreted from insulinoma NIT-1 cells. |
Authors |
Lee HS, Jeong J, Lee KJ. |
Journal name |
J Proteome Res
|
Publication year |
2009 |
Sample |
Pancreatic cells |
Sample name |
Pancreatic beta cell (NIT-1) |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|