Gene description for Rps6
Gene name ribosomal protein S6
Gene symbol Rps6
Other names/aliases S6R
Species Mus musculus
 Database cross references - Rps6
ExoCarta ExoCarta_20104
Vesiclepedia VP_20104
Entrez Gene 20104
UniProt P62754  
 Rps6 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rps6
Molecular Function
    mRNA binding GO:0003729 ISO
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    protein binding GO:0005515 IPI
    protein kinase binding GO:0019901 IEA
    protein kinase binding GO:0019901 ISO
Biological Process
    ribosomal small subunit assembly GO:0000028 ISO
    G1/S transition of mitotic cell cycle GO:0000082 IMP
    mitotic cell cycle GO:0000278 IMP
    placenta development GO:0001890 IMP
    cytoplasmic translation GO:0002181 ISO
    cytoplasmic translation GO:0002181 ISS
    cytoplasmic translation GO:0002181 NAS
    T cell proliferation involved in immune response GO:0002309 IGI
    T cell proliferation involved in immune response GO:0002309 IMP
    rRNA processing GO:0006364 IMP
    rRNA processing GO:0006364 ISO
    activation-induced cell death of T cells GO:0006924 IMP
    mitotic cell cycle checkpoint signaling GO:0007093 IMP
    gastrulation GO:0007369 IGI
    gastrulation GO:0007369 IMP
    positive regulation of cell population proliferation GO:0008284 ISO
    positive regulation of cell population proliferation GO:0008284 ISS
    mammalian oogenesis stage GO:0022605 IMP
    TOR signaling GO:0031929 IEA
    TOR signaling GO:0031929 ISO
    response to insulin GO:0032868 ISO
    T cell differentiation in thymus GO:0033077 IGI
    T cell differentiation in thymus GO:0033077 IMP
    ribosomal small subunit biogenesis GO:0042274 IMP
    ribosomal small subunit biogenesis GO:0042274 ISO
    ribosomal small subunit biogenesis GO:0042274 ISS
    glucose homeostasis GO:0042593 IDA
    positive regulation of apoptotic process GO:0043065 IEA
    positive regulation of apoptotic process GO:0043065 ISO
    negative regulation of apoptotic process GO:0043066 IMP
    erythrocyte development GO:0048821 IMP
    cellular response to ethanol GO:0071361 ISO
    negative regulation of bicellular tight junction assembly GO:1903347 ISO
Subcellular Localization
    nucleus GO:0005634 ISO
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 NAS
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 TAS
    ribosome GO:0005840 IDA
    ribosome GO:0005840 ISO
    small ribosomal subunit GO:0015935 ISO
    cytosolic ribosome GO:0022626 ISO
    cytosolic ribosome GO:0022626 ISO
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytosolic small ribosomal subunit GO:0022627 ISO
    cytosolic small ribosomal subunit GO:0022627 NAS
    dendrite GO:0030425 IDA
    dendrite GO:0030425 ISO
    small-subunit processome GO:0032040 ISO
    small-subunit processome GO:0032040 ISS
    cytoplasmic ribonucleoprotein granule GO:0036464 IEA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    cell body GO:0044297 IEA
    cell body GO:0044297 ISO
    perinuclear region of cytoplasm GO:0048471 IDA
    presynapse GO:0098793 ISO
    GABA-ergic synapse GO:0098982 ISO
    ribonucleoprotein complex GO:1990904 ISO
 Experiment description of studies that identified Rps6 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rps6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Rpl28 19943
Co-fractionation Mus musculus
3 Atxn2 20239
Co-fractionation Mus musculus
4 Rpl27 19942
Co-fractionation Mus musculus
5 Ybx1 22608
Co-fractionation Mus musculus
6 Fmr1  
Co-fractionation Mus musculus
7 Rpl6 19988
Co-fractionation Mus musculus
8 Rps24 20088
Co-fractionation Mus musculus
9 Ncl 17975
Co-fractionation Mus musculus
10 Actb 11461
Co-fractionation Mus musculus
11 Rpl35a 57808
Co-fractionation Mus musculus
12 Tmem173  
Proximity Label-MS Mus musculus
13 Rpl7 19989
Co-fractionation Mus musculus
14 Plod3 26433
Co-fractionation Mus musculus
15 P4hb 18453
Co-fractionation Mus musculus
16 Ina  
Co-fractionation Mus musculus
17 Rpl36a 19982
Co-fractionation Mus musculus
18 Rpl26 19941
Co-fractionation Mus musculus
19 Ctsh  
Co-fractionation Mus musculus
20 Rpsa 16785
Co-fractionation Mus musculus
21 Mapt  
Co-fractionation Mus musculus
22 Fxr1 14359
Co-fractionation Mus musculus
23 Rps10 67097
Co-fractionation Mus musculus
24 Sarnp 66118
Co-fractionation Mus musculus
25 Stau1  
Co-fractionation Mus musculus
26 Rplp2 67186
Co-fractionation Mus musculus
27 Rps4x 20102
Co-fractionation Mus musculus
28 Rps23 66475
Co-fractionation Mus musculus
29 Rpl14 67115
Co-fractionation Mus musculus
30 Scyl2  
Co-fractionation Mus musculus
31 Vars 22321
Co-fractionation Mus musculus
32 Tfe3  
Affinity Capture-MS Mus musculus
33 Rpl24 68193
Co-fractionation Mus musculus
34 Lsm14a  
Co-fractionation Mus musculus
35 Rps7 20115
Co-fractionation Mus musculus
36 Pabpc1 18458
Co-fractionation Mus musculus
37 Rps5 20103
Co-fractionation Mus musculus
38 Rps15a 267019
Co-fractionation Mus musculus
39 Rock2  
Co-fractionation Mus musculus
40 Snd1 56463
Co-fractionation Mus musculus
41 Poldip3  
Co-fractionation Mus musculus
42 Fancd2  
Affinity Capture-MS Mus musculus
43 Dhx9 13211
Co-fractionation Mus musculus
44 Rpl30 19946
Co-fractionation Mus musculus
45 Rps26 27370
Co-fractionation Mus musculus
46 Rac1 19353
Co-fractionation Mus musculus
47 Rps16 20055
Co-fractionation Mus musculus
48 Zfp330  
Co-fractionation Mus musculus
49 Hba 15121
Co-fractionation Mus musculus
50 Dhx29  
Co-fractionation Mus musculus
51 Rps18 20084
Co-fractionation Mus musculus
52 Rpl13a 22121
Co-fractionation Mus musculus
53 Psmc2 19181
Co-fractionation Mus musculus
54 Rps2 16898
Co-fractionation Mus musculus
55 Rps8 20116
Co-fractionation Mus musculus
56 Pde12  
Co-fractionation Mus musculus
57 Rps28 54127
Co-fractionation Mus musculus
58 Rps19 20085
Co-fractionation Mus musculus
59 Tpt1 22070
Co-fractionation Mus musculus
60 Rps11 27207
Co-fractionation Mus musculus
61 Pcbp1 23983
Co-fractionation Mus musculus
62 Lrrc59  
Co-fractionation Mus musculus
63 Rpl22 19934
Co-fractionation Mus musculus
64 Rplp0 11837
Co-fractionation Mus musculus
65 Syncrip 56403
Co-fractionation Mus musculus
66 Rps3a1 20091
Co-fractionation Mus musculus
67 Rps21 66481
Co-fractionation Mus musculus
68 Rps14 20044
Co-fractionation Mus musculus
69 Rps9 76846
Co-fractionation Mus musculus
70 Eed  
Affinity Capture-MS Mus musculus
71 Dhfr 13361
Co-fractionation Mus musculus
72 Rps20 67427
Co-fractionation Mus musculus
73 Rps12 20042
Co-fractionation Mus musculus
74 Ebna1bp2  
Co-fractionation Mus musculus
75 Rps25 75617
Co-fractionation Mus musculus
76 Mcts1  
Co-fractionation Mus musculus
77 Rps6kb1  
Biochemical Activity Mus musculus
78 Rpl10a 19896
Co-fractionation Mus musculus
79 Kctd13  
Affinity Capture-MS Mus musculus
80 Rpgrip1l  
Affinity Capture-MS Mus musculus
81 Ddx6 13209
Co-fractionation Mus musculus
82 Prpf3 70767
Co-fractionation Mus musculus
83 Mettl21e  
Affinity Capture-MS Mus musculus
84 Eef2 13629
Co-fractionation Mus musculus
85 Pa2g4 18813
Co-fractionation Mus musculus
86 Rpl18 19899
Co-fractionation Mus musculus
87 Gnb2l1 14694
Co-fractionation Mus musculus
88 Rps15 20054
Co-fractionation Mus musculus
89 Npm1 18148
Affinity Capture-MS Mus musculus
90 Rps13 68052
Co-fractionation Mus musculus
91 Rpl23 65019
Co-fractionation Mus musculus
92 Rpl15 66480
Co-fractionation Mus musculus
93 Top1 21969
Co-fractionation Mus musculus
94 Rpl4 67891
Co-fractionation Mus musculus
95 Rpl8 26961
Co-fractionation Mus musculus
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