Gene description for Rps4x
Gene name ribosomal protein S4, X-linked
Gene symbol Rps4x
Other names/aliases Rps4
Rps4-1
Species Mus musculus
 Database cross references - Rps4x
ExoCarta ExoCarta_20102
Vesiclepedia VP_20102
Entrez Gene 20102
UniProt P62702  
 Rps4x identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Fibroblasts 23260141    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rps4x
Molecular Function
    RNA binding GO:0003723 IBA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    rRNA binding GO:0019843 IEA
Biological Process
    cytoplasmic translation GO:0002181 NAS
    translation GO:0006412 IBA
    positive regulation of cell population proliferation GO:0008284 IEA
    ribosomal small subunit biogenesis GO:0042274 ISS
    positive regulation of translation GO:0045727 IEA
Subcellular Localization
    nucleolus GO:0005730 IEA
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 TAS
    ribosome GO:0005840 ISO
    ribosome GO:0005840 ISS
    small ribosomal subunit GO:0015935 ISA
    cytosolic ribosome GO:0022626 ISO
    cytosolic small ribosomal subunit GO:0022627 IBA
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytosolic small ribosomal subunit GO:0022627 ISO
    cytosolic small ribosomal subunit GO:0022627 NAS
    small-subunit processome GO:0032040 ISO
    small-subunit processome GO:0032040 ISS
    cytoplasmic ribonucleoprotein granule GO:0036464 IEA
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    ribonucleoprotein complex GO:1990904 ISO
    ribonucleoprotein complex GO:1990904 ISS
 Experiment description of studies that identified Rps4x in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 210
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|FLOT1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23260141    
Organism Mus musculus
Experiment description Exosomes Mediate Stromal Mobilization of Autocrine Wnt-PCP Signaling in Breast Cancer Cell Migration.
Authors "Luga V, Zhang L, Viloria-Petit AM, Ogunjimi AA, Inanlou MR, Chiu E, Buchanan M, Hosein AN, Basik M, Wrana JL."
Journal name Cell
Publication year 2012
Sample Fibroblasts
Sample name Normal-Fibroblasts (L cells)
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
5
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rps4x
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Ola1 67059
Co-fractionation Mus musculus
3 Herc4  
Co-fractionation Mus musculus
4 AI314180 230249
Co-fractionation Mus musculus
5 Atxn2 20239
Co-fractionation Mus musculus
6 Raver1  
Co-fractionation Mus musculus
7 Ybx1 22608
Co-fractionation Mus musculus
8 Fmr1  
Co-fractionation Mus musculus
9 Rpl6 19988
Co-fractionation Mus musculus
10 Cebpa  
Affinity Capture-MS Mus musculus
11 Rps24 20088
Co-fractionation Mus musculus
12 Ncl 17975
Co-fractionation Mus musculus
13 Actb 11461
Co-fractionation Mus musculus
14 Rpl35a 57808
Co-fractionation Mus musculus
15 Tmem173  
Proximity Label-MS Mus musculus
16 Eef1a1 13627
Co-fractionation Mus musculus
17 Rps9 76846
Co-fractionation Mus musculus
18 Dync1i1  
Co-fractionation Mus musculus
19 Ina  
Co-fractionation Mus musculus
20 Atp9a  
Co-fractionation Mus musculus
21 Rpl26 19941
Co-fractionation Mus musculus
22 Rpsa 16785
Co-fractionation Mus musculus
23 Rpl3 27367
Co-fractionation Mus musculus
24 Abce1 24015
Co-fractionation Mus musculus
25 Hnrnpa2b1 53379
Co-fractionation Mus musculus
26 Rpl11 67025
Co-fractionation Mus musculus
27 Eed  
Affinity Capture-MS Mus musculus
28 Rpl31 114641
Co-fractionation Mus musculus
29 Rps10 67097
Co-fractionation Mus musculus
30 Sarnp 66118
Co-fractionation Mus musculus
31 Eif5a 276770
Co-fractionation Mus musculus
32 Stau1  
Co-fractionation Mus musculus
33 Rplp2 67186
Co-fractionation Mus musculus
34 Wibg  
Co-fractionation Mus musculus
35 Trove2 20822
Co-fractionation Mus musculus
36 Rps23 66475
Co-fractionation Mus musculus
37 Rpl14 67115
Co-fractionation Mus musculus
38 Mapt  
Co-fractionation Mus musculus
39 Tfe3  
Affinity Capture-MS Mus musculus
40 Rpl24 68193
Co-fractionation Mus musculus
41 Rps7 20115
Co-fractionation Mus musculus
42 Pabpc1 18458
Co-fractionation Mus musculus
43 Gtf2f1  
Co-fractionation Mus musculus
44 Rps5 20103
Co-fractionation Mus musculus
45 Actn4 60595
Co-fractionation Mus musculus
46 Rps15a 267019
Co-fractionation Mus musculus
47 Rock2  
Co-fractionation Mus musculus
48 Ppwd1  
Co-fractionation Mus musculus
49 Poldip3  
Co-fractionation Mus musculus
50 Rps20 67427
Co-fractionation Mus musculus
51 Rplp0 11837
Co-fractionation Mus musculus
52 Rps6 20104
Co-fractionation Mus musculus
53 Rpl30 19946
Co-fractionation Mus musculus
54 Rpl39 67248
Co-fractionation Mus musculus
55 Rac1 19353
Co-fractionation Mus musculus
56 Rps16 20055
Co-fractionation Mus musculus
57 Zfp330  
Co-fractionation Mus musculus
58 Hba 15121
Co-fractionation Mus musculus
59 Sf3a3 75062
Co-fractionation Mus musculus
60 Dhx29  
Co-fractionation Mus musculus
61 Rps18 20084
Co-fractionation Mus musculus
62 Rpl13a 22121
Co-fractionation Mus musculus
63 Psmc2 19181
Co-fractionation Mus musculus
64 Rps2 16898
Co-fractionation Mus musculus
65 Gapdh 14433
Co-fractionation Mus musculus
66 Rps8 20116
Co-fractionation Mus musculus
67 Rpl7a 27176
Co-fractionation Mus musculus
68 Rps28 54127
Co-fractionation Mus musculus
69 Rps19 20085
Co-fractionation Mus musculus
70 Calr 12317
Co-fractionation Mus musculus
71 Pkm 18746
Co-fractionation Mus musculus
72 Tpt1 22070
Co-fractionation Mus musculus
73 Rps11 27207
Co-fractionation Mus musculus
74 Rpl18a 76808
Co-fractionation Mus musculus
75 Ago1 236511
Co-fractionation Mus musculus
76 Pcbp1 23983
Co-fractionation Mus musculus
77 Lrrc59  
Co-fractionation Mus musculus
78 Rpl22 19934
Co-fractionation Mus musculus
79 Cbx5  
Affinity Capture-MS Mus musculus
80 Rpl38  
Co-fractionation Mus musculus
81 Syncrip 56403
Co-fractionation Mus musculus
82 Rps3a1 20091
Co-fractionation Mus musculus
83 Rps21 66481
Co-fractionation Mus musculus
84 Rps14 20044
Co-fractionation Mus musculus
85 Rpl35 66489
Co-fractionation Mus musculus
86 Drg1  
Co-fractionation Mus musculus
87 Tia1  
Affinity Capture-MS Mus musculus
88 Rpl5 100503670
Co-fractionation Mus musculus
89 Fancd2  
Affinity Capture-MS Mus musculus
90 Rps12 20042
Co-fractionation Mus musculus
91 Rps27a 78294
Co-fractionation Mus musculus
92 Ubr4 69116
Co-fractionation Mus musculus
93 Ncbp1 433702
Co-fractionation Mus musculus
94 Rps25 75617
Co-fractionation Mus musculus
95 Tubb5 22154
Co-fractionation Mus musculus
96 Top1 21969
Co-fractionation Mus musculus
97 Kctd13  
Affinity Capture-MS Mus musculus
98 Rpgrip1l  
Affinity Capture-MS Mus musculus
99 Ddx6 13209
Co-fractionation Mus musculus
100 Prpf3 70767
Co-fractionation Mus musculus
101 Fzr1  
Affinity Capture-MS Mus musculus
102 Eef2 13629
Co-fractionation Mus musculus
103 Rpl12 269261
Co-fractionation Mus musculus
104 Rpl18 19899
Co-fractionation Mus musculus
105 Dhx9 13211
Co-fractionation Mus musculus
106 Gnb2l1 14694
Co-fractionation Mus musculus
107 Rps15 20054
Co-fractionation Mus musculus
108 Plod3 26433
Co-fractionation Mus musculus
109 Rps13 68052
Co-fractionation Mus musculus
110 Rps17 20068
Co-fractionation Mus musculus
111 Rps26 27370
Co-fractionation Mus musculus
112 Rpl23a 268449
Co-fractionation Mus musculus
113 Rpl15 66480
Co-fractionation Mus musculus
114 Eef1b2 55949
Co-fractionation Mus musculus
115 Rpl13 270106
Co-fractionation Mus musculus
116 Rpl8 26961
Co-fractionation Mus musculus
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