Experiment description of studies that identified EPN3 in exosomes

1 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

2 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 63 MISEV standards ✘ Biophysical techniques ✔ AQP2 Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 19056867     Organism Homo sapiens Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes. Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA" Journal name JASN Publication year 2009 Sample Urine Sample name Urine - Normal Isolation/purification methods Differential centrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [LTQ] Western blotting

Protein-protein interactions for EPN3

Protein Interactor ExoCarta ID Identification method PubMed Species 1 SHKBP1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 2 RALBP1 10928 Affinity Capture-Western Homo sapiens 3 CCDC130   Affinity Capture-MS Homo sapiens 4 MIB1 57534 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 5 EHD1 10938 Affinity Capture-Western Homo sapiens 6 RNF11 26994 Two-hybrid Homo sapiens 7 KCTD10 83892 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 TNKS 8658 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 9 RBM47 54502 Affinity Capture-MS Homo sapiens 10 TNFAIP1 7126 Affinity Capture-MS Homo sapiens 11 TPD52L1 7164 Affinity Capture-MS Homo sapiens 12 CMPK1 51727 Co-fractionation Homo sapiens 13 HSPA1L 3305 Affinity Capture-MS Homo sapiens 14 EPS15 2060 Affinity Capture-Western Homo sapiens 15 CASP2   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 16 SMURF1 57154 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 17 INSIG2   Affinity Capture-MS Homo sapiens 18 TNKS2 80351 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 19 MID1 4281 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 20 EPN2 22905 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 FEM1B 10116 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 22 EHD2 30846 Affinity Capture-Western Homo sapiens 23 ZC3H10   Affinity Capture-MS Homo sapiens 24 LAPTM5 7805 Two-hybrid Homo sapiens 25 C10orf88   Affinity Capture-MS Homo sapiens 26 BTBD1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 UBR1 197131 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 28 CDH1 999 Proximity Label-MS Homo sapiens 29 UBB 7314 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 30 RTCB 51493 Affinity Capture-MS Homo sapiens 31 CYHR1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 32 EPN1 29924 Co-localization Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 DTX2 113878 Proximity Label-MS Homo sapiens 34 TRIM32 22954 Affinity Capture-MS Homo sapiens 35 ARIH1 25820 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 36 VPS39 23339 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 37 BTBD9   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 38 KCTD3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 39 MID2 11043 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 40 PHGDH 26227 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 41 CDC27 996 Affinity Capture-MS Homo sapiens View the network

Pathways in which EPN3 is involved

No pathways found