Gene description for PANK2
Gene name pantothenate kinase 2
Gene symbol PANK2
Other names/aliases C20orf48
HARP
HSS
NBIA1
PKAN
Species Homo sapiens
 Database cross references - PANK2
ExoCarta ExoCarta_80025
Vesiclepedia VP_80025
Entrez Gene 80025
HGNC 15894
MIM 606157
UniProt Q9BZ23  
 PANK2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Thymus 23844026    
 Gene ontology annotations for PANK2
Molecular Function
    pantothenate kinase activity GO:0004594 IBA
    pantothenate kinase activity GO:0004594 IMP
    pantothenate kinase activity GO:0004594 NAS
    pantothenate kinase activity GO:0004594 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
Biological Process
    angiogenesis GO:0001525 IMP
    mitochondrion organization GO:0007005 IEA
    spermatid development GO:0007286 IEA
    aerobic respiration GO:0009060 IEA
    coenzyme A biosynthetic process GO:0015937 IBA
    coenzyme A biosynthetic process GO:0015937 IEA
    coenzyme A biosynthetic process GO:0015937 NAS
    coenzyme A biosynthetic process GO:0015937 TAS
    pantothenate metabolic process GO:0015939 NAS
    phosphorylation GO:0016310 NAS
    regulation of fatty acid metabolic process GO:0019217 IMP
    regulation of mitochondrial membrane potential GO:0051881 IEA
    regulation of triglyceride metabolic process GO:0090207 IMP
    regulation of bile acid metabolic process GO:1904251 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrion GO:0005739 NAS
    mitochondrial intermembrane space GO:0005758 IDA
    mitochondrial intermembrane space GO:0005758 TAS
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified PANK2 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
3
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PANK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAB 7529
Affinity Capture-MS Homo sapiens
2 YWHAH 7533
Affinity Capture-MS Homo sapiens
3 LRFN1  
Affinity Capture-MS Homo sapiens
4 WDR6 11180
Affinity Capture-MS Homo sapiens
5 PVRL2 5819
Affinity Capture-MS Homo sapiens
6 GIGYF1  
Affinity Capture-MS Homo sapiens
7 KSR1  
Affinity Capture-MS Homo sapiens
8 DHX36 170506
Affinity Capture-MS Homo sapiens
9 HSPA8 3312
Affinity Capture-MS Homo sapiens
10 LXN 56925
Affinity Capture-MS Homo sapiens
11 YWHAE 7531
Affinity Capture-MS Homo sapiens
12 RASGRF2  
Affinity Capture-MS Homo sapiens
13 YWHAZ 7534
Affinity Capture-MS Homo sapiens
14 ZNF526  
Affinity Capture-MS Homo sapiens
15 CBY1  
Affinity Capture-MS Homo sapiens
16 KIF13B 23303
Affinity Capture-MS Homo sapiens
17 UBB 7314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 SAT2 112483
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 MKRN2 23609
Affinity Capture-RNA Homo sapiens
20 ZBTB21  
Affinity Capture-MS Homo sapiens
21 QRICH2  
Affinity Capture-MS Homo sapiens
22 CGN  
Affinity Capture-MS Homo sapiens
23 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SCD 6319
Affinity Capture-MS Homo sapiens
25 VDAC1 7416
Affinity Capture-MS Homo sapiens
26 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 KCTD3  
Affinity Capture-MS Homo sapiens
28 PANK3 79646
Affinity Capture-MS Homo sapiens
29 TACC3  
Affinity Capture-MS Homo sapiens
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