Gene description for TNFRSF10A
Gene name tumor necrosis factor receptor superfamily, member 10a
Gene symbol TNFRSF10A
Other names/aliases APO2
CD261
DR4
TRAILR-1
TRAILR1
Species Homo sapiens
 Database cross references - TNFRSF10A
ExoCarta ExoCarta_8797
Vesiclepedia VP_8797
Entrez Gene 8797
HGNC 11904
MIM 603611
UniProt O00220  
 TNFRSF10A identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 25844599    
 Gene ontology annotations for TNFRSF10A
Molecular Function
    protease binding GO:0002020 IPI
    death receptor activity GO:0005035 TAS
    protein binding GO:0005515 IPI
    signaling receptor activity GO:0038023 NAS
    identical protein binding GO:0042802 IDA
    TRAIL binding GO:0045569 NAS
Biological Process
    apoptotic process GO:0006915 NAS
    signal transduction GO:0007165 TAS
    activation of NF-kappaB-inducing kinase activity GO:0007250 NAS
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 NAS
    TRAIL-activated apoptotic signaling pathway GO:0036462 IBA
    TRAIL-activated apoptotic signaling pathway GO:0036462 IDA
    TRAIL-activated apoptotic signaling pathway GO:0036462 IMP
    positive regulation of apoptotic process GO:0043065 IBA
    cellular response to mechanical stimulus GO:0071260 IEP
    extrinsic apoptotic signaling pathway GO:0097191 IDA
Subcellular Localization
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    cell surface GO:0009986 IBA
    cell surface GO:0009986 IDA
    plasma membrane raft GO:0044853 IDA
    membrane raft GO:0045121 IDA
 Experiment description of studies that identified TNFRSF10A in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for TNFRSF10A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RNF149 284996
Affinity Capture-MS Homo sapiens
2 SLC4A7 9497
Affinity Capture-MS Homo sapiens
3 MPP1 4354
Affinity Capture-MS Homo sapiens
4 TNFAIP3 7128
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
5 PPP1R15B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 FADD 8772
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
7 LAPTM4B  
Affinity Capture-MS Homo sapiens
8 SDCBP 6386
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 ACVR2A  
Affinity Capture-MS Homo sapiens
10 TRAF2 7186
Affinity Capture-Western Homo sapiens
11 PTPRD 5789
Affinity Capture-MS Homo sapiens
12 CFLAR  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 TGFBR2 7048
Affinity Capture-MS Homo sapiens
14 CD320 51293
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 TNFSF10 8743
Affinity Capture-Western Homo sapiens
16 DAP3 7818
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
17 FGFR3 2261
Affinity Capture-MS Homo sapiens
18 RAB9A 9367
Affinity Capture-MS Homo sapiens
19 MARCH1  
Affinity Capture-Western Homo sapiens
20 TMEM214 54867
Affinity Capture-MS Homo sapiens
21 NDFIP1 80762
Affinity Capture-MS Homo sapiens
22 ATG9A 79065
Affinity Capture-MS Homo sapiens
23 ATP11A 23250
Affinity Capture-MS Homo sapiens
24 IL4R  
Affinity Capture-MS Homo sapiens
25 MARCH8  
Affinity Capture-Western Homo sapiens
26 CD70 970
Affinity Capture-MS Homo sapiens
27 GYG2  
Affinity Capture-MS Homo sapiens
28 BMPR1A 657
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 CASP8 841
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
30 S100A10 6281
Affinity Capture-MS Homo sapiens
31 CUL3 8452
Affinity Capture-Western Homo sapiens
32 TRADD 8717
Affinity Capture-Western Homo sapiens
33 SQSTM1 8878
Affinity Capture-Western Homo sapiens
34 CDC6  
Affinity Capture-MS Homo sapiens
35 UGCG 7357
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 BTN2A2  
Affinity Capture-MS Homo sapiens
37 PLEKHB2 55041
Affinity Capture-MS Homo sapiens
38 LSS 4047
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 BCL10  
Two-hybrid Homo sapiens
40 PI4K2A 55361
Affinity Capture-MS Homo sapiens
41 LRP10 26020
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 ACVR1 90
Affinity Capture-MS Homo sapiens
43 CBL 867
Affinity Capture-Western Homo sapiens
44 APEX1 328
Affinity Capture-RNA Homo sapiens
45 UBB 7314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 TNFRSF10B 8795
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
47 HLA-DQA1 3117
Affinity Capture-MS Homo sapiens
48 CLCN7 1186
Affinity Capture-MS Homo sapiens
49 BTN2A1 11120
Affinity Capture-MS Homo sapiens
50 C9orf41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 RELT  
Affinity Capture-MS Homo sapiens
52 SPRTN  
Affinity Capture-MS Homo sapiens
53 KCNT2  
Affinity Capture-MS Homo sapiens
54 ST7L  
Affinity Capture-MS Homo sapiens
55 ADCY6 112
Affinity Capture-MS Homo sapiens
56 CCDC107  
Affinity Capture-MS Homo sapiens
57 CASP10  
Affinity Capture-Western Homo sapiens
58 ALDH3B1 221
Affinity Capture-MS Homo sapiens
59 DDRGK1 65992
Affinity Capture-MS Homo sapiens
60 FGFR4 2264
Affinity Capture-MS Homo sapiens
61 GPRC5B 51704
Affinity Capture-MS Homo sapiens
62 IFNAR1  
Affinity Capture-MS Homo sapiens
63 SLC12A4 6560
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 ERMP1 79956
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
65 ABCD4 5826
Affinity Capture-MS Homo sapiens
66 PIGH  
Affinity Capture-MS Homo sapiens
67 KIAA0195 9772
Affinity Capture-MS Homo sapiens
68 STEAP3 55240
Affinity Capture-MS Homo sapiens
69 FBXO11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 C3orf52  
Affinity Capture-MS Homo sapiens
71 MFAP3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 MAP1LC3B2  
Affinity Capture-MS Homo sapiens
73 PCDHB3  
Affinity Capture-MS Homo sapiens
74 TNFSF9  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here