Gene description for SNAP91
Gene name synaptosomal-associated protein, 91kDa
Gene symbol SNAP91
Other names/aliases AP180
CALM
Species Homo sapiens
 Database cross references - SNAP91
ExoCarta ExoCarta_9892
Vesiclepedia VP_9892
Entrez Gene 9892
HGNC 14986
MIM 607923
UniProt O60641  
 SNAP91 identified in exosomes derived from the following tissue/cell type
Chondrocytes 35931686    
Chondrocytes 35931686    
 Gene ontology annotations for SNAP91
Molecular Function
    SNARE binding GO:0000149 IBA
    protein binding GO:0005515 IPI
    1-phosphatidylinositol binding GO:0005545 IBA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IBA
    protein kinase binding GO:0019901 ISS
    clathrin heavy chain binding GO:0032050 IBA
Biological Process
    protein transport GO:0015031 IEA
    synaptic vesicle budding from presynaptic endocytic zone membrane GO:0016185 IBA
    clathrin coat assembly GO:0048268 IEA
    clathrin-dependent endocytosis GO:0072583 IBA
    regulation of clathrin-dependent endocytosis GO:2000369 IMP
Subcellular Localization
    clathrin-coated pit GO:0005905 IBA
    synaptic vesicle GO:0008021 IBA
    clathrin-coated vesicle GO:0030136 IBA
    extrinsic component of presynaptic endocytic zone membrane GO:0098894 IBA
 Experiment description of studies that identified SNAP91 in exosomes
1
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SNAP91
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CLTC 1213
Affinity Capture-Western Homo sapiens
2 CDCA8 55143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 MIB1 57534
Affinity Capture-MS Homo sapiens
4 DNAAF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 GMNN  
Affinity Capture-MS Homo sapiens
6 NTMT1 28989
Affinity Capture-MS Homo sapiens
7 CLTCL1 8218
Affinity Capture-MS Homo sapiens
8 ATG9A 79065
Proximity Label-MS Homo sapiens
9 ASNS 440
Affinity Capture-MS Homo sapiens
10 GPM6A 2823
Affinity Capture-MS Homo sapiens
11 RABGEF1 27342
Affinity Capture-MS Homo sapiens
12 TRAF3IP1 26146
Two-hybrid Homo sapiens
13 MIS18A  
Affinity Capture-MS Homo sapiens
14 BRCA1 672
Affinity Capture-MS Homo sapiens
15 BRIP1  
Affinity Capture-MS Homo sapiens
16 EPS15 2060
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
17 RB1CC1 9821
Affinity Capture-MS Homo sapiens
18 CAMSAP2  
Affinity Capture-MS Homo sapiens
19 SENP3 26168
Affinity Capture-MS Homo sapiens
20 SETDB1  
Affinity Capture-MS Homo sapiens
21 ACIN1 22985
Co-fractionation Homo sapiens
22 MLPH  
Affinity Capture-MS Homo sapiens
23 UBB 7314
Affinity Capture-MS Homo sapiens
24 ARF6 382
Affinity Capture-Western Homo sapiens
25 ANKRD52 283373
Affinity Capture-MS Homo sapiens
26 TRIM44  
Affinity Capture-MS Homo sapiens
27 VCL 7414
Affinity Capture-MS Homo sapiens
28 TTN 7273
Cross-Linking-MS (XL-MS) Homo sapiens
29 DGUOK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 NUFIP1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 AP1B1 162
Affinity Capture-Western Homo sapiens
32 PEX5 5830
Affinity Capture-MS Homo sapiens
33 PCM1 5108
Affinity Capture-MS Homo sapiens
34 HAX1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 RABEP1 9135
Affinity Capture-MS Homo sapiens
36 PICALM 8301
Affinity Capture-MS Homo sapiens
37 DCAF5  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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