Gene description for UBE2G1
Gene name ubiquitin-conjugating enzyme E2G 1
Gene symbol UBE2G1
Other names/aliases E217K
UBC7
UBE2G
Species Homo sapiens
 Database cross references - UBE2G1
ExoCarta ExoCarta_7326
Vesiclepedia VP_7326
Entrez Gene 7326
HGNC 12482
MIM 601569
UniProt P62253  
 UBE2G1 identified in sEVs derived from the following tissue/cell type
Endothelial cells 26027894    
Urine 19056867    
 Gene ontology annotations for UBE2G1
Molecular Function
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    ubiquitin protein ligase binding GO:0031625 IPI
    ubiquitin conjugating enzyme activity GO:0061631 IBA
    ubiquitin conjugating enzyme activity GO:0061631 IDA
Biological Process
    protein polyubiquitination GO:0000209 IBA
    ubiquitin-dependent protein catabolic process GO:0006511 TAS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    protein K63-linked ubiquitination GO:0070534 IDA
    protein K48-linked ubiquitination GO:0070936 IDA
Subcellular Localization
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified UBE2G1 in sEVs
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for UBE2G1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRBN  
Reconstituted Complex Homo sapiens
2 APOA1BP 128240
Co-fractionation Homo sapiens
3 CYP3A4  
Affinity Capture-MS Homo sapiens
4 Tmed10 68581
Affinity Capture-MS Mus musculus
5 RNF11 26994
Affinity Capture-MS Homo sapiens
6 UNK  
Affinity Capture-RNA Homo sapiens
7 IL36G 56300
Affinity Capture-MS Homo sapiens
8 DIO2  
FRET Homo sapiens
Reconstituted Complex Homo sapiens
9 UBA1 7317
Reconstituted Complex Homo sapiens
10 UBA52 7311
Affinity Capture-MS Homo sapiens
11 UBC 7316
Reconstituted Complex Homo sapiens
12 SMURF1 57154
Biochemical Activity Homo sapiens
13 RBX1 9978
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
14 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
15 MAP3K1 4214
Biochemical Activity Homo sapiens
16 PARK2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
17 AMFR 267
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
18 APP 351
Reconstituted Complex Homo sapiens
19 USP8 9101
Co-fractionation Homo sapiens
20 RDH5 5959
Affinity Capture-MS Homo sapiens
21 WWP2 11060
Co-fractionation Homo sapiens
22 CUL9  
Reconstituted Complex Homo sapiens
23 Trim63  
Reconstituted Complex Rattus norvegicus
Two-hybrid Rattus norvegicus
PCA Rattus norvegicus
24 MARCH6  
Reconstituted Complex Homo sapiens
25 UBE2D3 7323
Phenotypic Enhancement Homo sapiens
26 RC3H1 149041
Reconstituted Complex Homo sapiens
27 UBE2G1 7326
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
28 ITCH 83737
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
29 RBCK1  
Two-hybrid Homo sapiens
30 RNF144A  
Affinity Capture-MS Homo sapiens
31 TRIM63  
Reconstituted Complex Homo sapiens
32 HUWE1 10075
Reconstituted Complex Homo sapiens
View the network image/svg+xml



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