Gene description for KRT36
Gene name keratin 36, type I
Gene symbol KRT36
Other names/aliases HA6
KRTHA6
hHa6
Species Homo sapiens
 Database cross references - KRT36
ExoCarta ExoCarta_8689
Vesiclepedia VP_8689
Entrez Gene 8689
HGNC 6454
MIM 604540
UniProt O76013  
 KRT36 identified in sEVs derived from the following tissue/cell type
Prostate cancer cells 25844599    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for KRT36
Molecular Function
    protein binding GO:0005515 IPI
    structural constituent of skin epidermis GO:0030280 IBA
    structural constituent of skin epidermis GO:0030280 NAS
Biological Process
    epithelial cell differentiation GO:0030855 IBA
    intermediate filament organization GO:0045109 IBA
    regulation of keratinocyte differentiation GO:0045616 IEA
Subcellular Localization
    cytosol GO:0005829 TAS
    keratin filament GO:0045095 IPI
    intermediate filament cytoskeleton GO:0045111 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified KRT36 in sEVs
1
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
2
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 193
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|CD9
Enriched markers
✔
PHB
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 194
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 195
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors "Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC."
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KRT36
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KRT76 51350
Two-hybrid Homo sapiens
2 KRT77 374454
Two-hybrid Homo sapiens
3 KRT85  
Two-hybrid Homo sapiens
4 KRT4 3851
Two-hybrid Homo sapiens
5 KRT73 319101
Two-hybrid Homo sapiens
6 ABI3 51225
Two-hybrid Homo sapiens
7 KRT3 3850
Two-hybrid Homo sapiens
8 EFNA4  
Affinity Capture-MS Homo sapiens
9 BCAS2 10286
Two-hybrid Homo sapiens
10 QARS 5859
Two-hybrid Homo sapiens
11 KRT1 3848
Two-hybrid Homo sapiens
12 GRB2 2885
Affinity Capture-MS Homo sapiens
13 MYOZ1  
Two-hybrid Homo sapiens
14 EXOC8 149371
Two-hybrid Homo sapiens
15 C1orf216  
Two-hybrid Homo sapiens
16 CCHCR1  
Two-hybrid Homo sapiens
17 KRT86  
Two-hybrid Homo sapiens
18 KRT6A 3853
Two-hybrid Homo sapiens
19 RIBC1  
Two-hybrid Homo sapiens
20 TSGA10IP  
Two-hybrid Homo sapiens
21 EPS15 2060
Affinity Capture-MS Homo sapiens
22 CUL3 8452
Affinity Capture-MS Homo sapiens
23 SMURF1 57154
Biochemical Activity Homo sapiens
24 TTC23  
Two-hybrid Homo sapiens
25 MAP3K1 4214
Biochemical Activity Homo sapiens
26 HCK 3055
Two-hybrid Homo sapiens
27 TTLL10 254173
Two-hybrid Homo sapiens
28 APP 351
Reconstituted Complex Homo sapiens
29 ZNF414  
Two-hybrid Homo sapiens
30 MYOZ3  
Two-hybrid Homo sapiens
31 KRT6C 286887
Two-hybrid Homo sapiens
32 USP8 9101
Affinity Capture-MS Homo sapiens
33 ABI2 10152
Two-hybrid Homo sapiens
34 OTUD4  
Affinity Capture-MS Homo sapiens
35 GEM  
Two-hybrid Homo sapiens
36 KRT80 144501
Two-hybrid Homo sapiens
37 KRT8 3856
Two-hybrid Homo sapiens
38 TWF2 11344
Affinity Capture-MS Homo sapiens
39 KRT75 9119
Two-hybrid Homo sapiens
40 DNPEP 23549
Affinity Capture-MS Homo sapiens
41 HGS 9146
Two-hybrid Homo sapiens
42 PPP1R18 170954
Two-hybrid Homo sapiens
43 PKN1 5585
Two-hybrid Homo sapiens
44 ZNF785  
Two-hybrid Homo sapiens
45 AMOT 154796
Two-hybrid Homo sapiens
46 TMSB4X 7114
Two-hybrid Homo sapiens
47 KRT2 3849
Two-hybrid Homo sapiens
48 CCBE1 147372
Two-hybrid Homo sapiens
49 KRT78 196374
Two-hybrid Homo sapiens
50 KRT79 338785
Two-hybrid Homo sapiens
51 AIMP2 7965
Two-hybrid Homo sapiens
52 KIAA1551  
Two-hybrid Homo sapiens
53 PLEKHN1  
Two-hybrid Homo sapiens
54 GPS2  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which KRT36 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





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