Gene description for ZFAND5
Gene name
zinc finger, AN1-type domain 5
Gene symbol
ZFAND5
Other names/aliases
ZA20D2 ZFAND5A ZNF216
Species
Homo sapiens
Database cross references - ZFAND5
ExoCarta
ExoCarta_7763
Entrez Gene
7763
HGNC
13008
MIM
604761
UniProt
O76080
ZFAND5 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells
26054723
Neuroblastoma cells
25944692
Gene ontology annotations for ZFAND5
Experiment description of studies that identified ZFAND5 in exosomes
1
Experiment ID
235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
2
Experiment ID
224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘
EV Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
Protein-protein interactions for ZFAND5
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
IKBKG
8517
Affinity Capture-MS
Homo sapiens
Affinity Capture-Western
Homo sapiens
2
TRAF6
7189
Affinity Capture-Western
Homo sapiens
View the network
image/svg+xml
Pathways in which ZFAND5 is involved
No pathways found