Gene description for MAPK9
Gene name mitogen-activated protein kinase 9
Gene symbol MAPK9
Other names/aliases JNK-55
JNK2
JNK2A
JNK2ALPHA
JNK2B
JNK2BETA
PRKM9
SAPK
SAPK1a
p54a
p54aSAPK
Species Homo sapiens
 Database cross references - MAPK9
ExoCarta ExoCarta_5601
Vesiclepedia VP_5601
Entrez Gene 5601
HGNC 6886
MIM 602896
UniProt P45984  
 MAPK9 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for MAPK9
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IDA
    JUN kinase activity GO:0004705 IBA
    JUN kinase activity GO:0004705 IDA
    JUN kinase activity GO:0004705 TAS
    protein serine/threonine/tyrosine kinase activity GO:0004712 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein serine kinase activity GO:0106310 IEA
Biological Process
    protein phosphorylation GO:0006468 IMP
    JNK cascade GO:0007254 IBA
    JNK cascade GO:0007254 IDA
    positive regulation of gene expression GO:0010628 IMP
    positive regulation of macrophage derived foam cell differentiation GO:0010744 IMP
    positive regulation of protein ubiquitination GO:0031398 IEA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IEA
    cellular response to reactive oxygen species GO:0034614 IDA
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    regulation of circadian rhythm GO:0042752 ISS
    response to cadmium ion GO:0046686 IEA
    rhythmic process GO:0048511 IEA
    modulation of chemical synaptic transmission GO:0050804 IEA
    protein localization to tricellular tight junction GO:0061833 IEA
    positive regulation of podosome assembly GO:0071803 IEA
    cellular senescence GO:0090398 TAS
    inflammatory response to wounding GO:0090594 IDA
    apoptotic signaling pathway GO:0097190 IEA
    positive regulation of cytokine production involved in inflammatory response GO:1900017 IDA
    positive regulation of apoptotic signaling pathway GO:2001235 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    mitochondrion GO:0005739 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    nuclear speck GO:0016607 IDA
    Schaffer collateral - CA1 synapse GO:0098685 IEA
 Experiment description of studies that identified MAPK9 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAPK9
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 TUSC2  
Two-hybrid Homo sapiens
2 PPP1R14D  
Affinity Capture-MS Homo sapiens
3 STK25 10494
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
4 RIN3  
Affinity Capture-MS Homo sapiens
5 DUSP8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 WNT7A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 TEX11  
Two-hybrid Homo sapiens
8 JUND 3727
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
9 CDC25B 994
Biochemical Activity Homo sapiens
10 HOXC10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 SRSF2 6427
Affinity Capture-MS Homo sapiens
12 NCF1  
Biochemical Activity Homo sapiens
13 PRKDC 5591
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
14 ZNF414  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 MAPK10 5602
Affinity Capture-MS Homo sapiens
16 DUSP14 11072
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 WDR62  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
18 ATXN7L1 222255
Affinity Capture-MS Homo sapiens
19 SH3BP5 9467
Reconstituted Complex Homo sapiens
20 DTX3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CTBP1 1487
Two-hybrid Homo sapiens
22 CASP3 836
Biochemical Activity Homo sapiens
23 SPANXN4  
Affinity Capture-MS Homo sapiens
24 NFE2L2 4780
Affinity Capture-Western Homo sapiens
25 NFATC3 4775
Two-hybrid Homo sapiens
26 ATF2  
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
27 XPO7 23039
Two-hybrid Homo sapiens
28 PPM1A 5494
Co-fractionation Homo sapiens
29 TULP3 7289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 CDT1  
Affinity Capture-Western Homo sapiens
31 LMAN2 10960
Two-hybrid Homo sapiens
32 MAPK8IP2  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
33 MAPK8IP3  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
34 HMGCS1 3157
Co-fractionation Homo sapiens
35 CLU 1191
Two-hybrid Homo sapiens
36 SHMT1 6470
Two-hybrid Homo sapiens
37 CCDC33 80125
Two-hybrid Homo sapiens
38 FAM9C  
Affinity Capture-MS Homo sapiens
39 MAPK8IP1 9479
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
40 FAM19A3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 ACP5  
Two-hybrid Homo sapiens
42 C9orf170  
Affinity Capture-MS Homo sapiens
43 Trp53  
Biochemical Activity Mus musculus
44 PKN1 5585
Co-fractionation Homo sapiens
45 CSNK1E 1454
Affinity Capture-MS Homo sapiens
46 ZFC3H1 196441
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 RASL10B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 MPDZ  
Affinity Capture-MS Homo sapiens
49 NFATC4 4776
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
50 MAP2K7 5609
Biochemical Activity Homo sapiens
Protein-peptide Homo sapiens
51 LRRC15 131578
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 MAPK9 5601
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
53 DUSP1  
Affinity Capture-Western Homo sapiens
54 SEC13 6396
Affinity Capture-MS Homo sapiens
55 CCDC120  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 DSG4 147409
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 CTNNB1 1499
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
58 HDAC9  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
59 PRKD1 5587
Affinity Capture-Western Homo sapiens
60 EGFR 1956
Two-hybrid Homo sapiens
PCA Homo sapiens
61 MAP3K1 4214
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
62 LAMA1 284217
Affinity Capture-MS Homo sapiens
63 KIAA1377  
Two-hybrid Homo sapiens
64 FTH1 2495
Affinity Capture-MS Homo sapiens
65 DUSP16  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 SERPINB5 5268
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 CDC42 998
Co-localization Homo sapiens
68 SMCO3  
Two-hybrid Homo sapiens
69 JUN 3725
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
70 ELK1  
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
71 HIST1H1B 3009
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 L3MBTL3  
Two-hybrid Homo sapiens
73 FOS 2353
Affinity Capture-MS Homo sapiens
74 C3orf18  
Affinity Capture-MS Homo sapiens
75 RPA3 6119
Proximity Label-MS Homo sapiens
76 MAPKAPK5 8550
Co-localization Homo sapiens
77 PRAMEF9  
Affinity Capture-MS Homo sapiens
78 C1orf94  
Two-hybrid Homo sapiens
79 ZBTB25  
Two-hybrid Homo sapiens
80 RHPN1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 JUNB  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
82 MFN2 9927
Affinity Capture-Western Homo sapiens
83 Rarg  
Reconstituted Complex Mus musculus
84 TOB1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
85 AKT1 207
Biochemical Activity Homo sapiens
86 NFATC2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
87 IRF3 3661
Affinity Capture-Western Homo sapiens
88 Rps6kb1  
Reconstituted Complex Mus musculus
89 PKP3 11187
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
90 Rara  
Reconstituted Complex Mus musculus
91 C6orf222  
Affinity Capture-MS Homo sapiens
92 CXorf66  
Affinity Capture-MS Homo sapiens
93 DUSP19  
Biochemical Activity Homo sapiens
94 SH3RF3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
95 ARRB2 409
Two-hybrid Homo sapiens
96 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
97 SPTAN1 6709
Affinity Capture-MS Homo sapiens
98 TRAF2 7186
Co-localization Homo sapiens
99 MAP2K4 6416
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
100 EFHC2  
Two-hybrid Homo sapiens
101 DUSP10 11221
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
102 GRB2 2885
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
103 LNX2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
104 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
105 C5orf24 134553
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 SSU72 29101
Two-hybrid Homo sapiens
107 LRRC56  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 MACF1 23499
Two-hybrid Homo sapiens
109 MAPK8 5599
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 VSIG8  
Affinity Capture-MS Homo sapiens
111 HDAC1 3065
Affinity Capture-Western Homo sapiens
112 STK26 51765
Co-fractionation Homo sapiens
113 BCL10  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
114 C19orf25  
Affinity Capture-MS Homo sapiens
115 CEP44  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
116 FAM110D  
Affinity Capture-MS Homo sapiens
117 SF3B4 10262
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
118 ARRB1 408
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
119 CYHR1  
Two-hybrid Homo sapiens
120 DHX33  
Affinity Capture-MS Homo sapiens
121 DUSP4 1846
Co-localization Homo sapiens
122 LNX1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
123 XPNPEP1 7511
Two-hybrid Homo sapiens
124 MEOX1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MAPK9 is involved
PathwayEvidenceSource
Activation of the AP-1 family of transcription factors TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cellular Senescence TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Fc epsilon receptor (FCERI) signaling TAS Reactome
FCERI mediated MAPK activation TAS Reactome
Immune System TAS Reactome
Innate Immune System TAS Reactome
Interleukin-17 signaling TAS Reactome
JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1 TAS Reactome
MAP kinase activation TAS Reactome
MAPK targets/ Nuclear events mediated by MAP kinases TAS Reactome
MyD88 cascade initiated on plasma membrane TAS Reactome
MyD88 dependent cascade initiated on endosome TAS Reactome
MyD88-independent TLR4 cascade TAS Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane TAS Reactome
Oxidative Stress Induced Senescence TAS Reactome
Signaling by ALK fusions and activated point mutants TAS Reactome
Signaling by ALK in cancer TAS Reactome
Signaling by Interleukins TAS Reactome
Toll Like Receptor 10 (TLR10) Cascade TAS Reactome
Toll Like Receptor 2 (TLR2) Cascade TAS Reactome
Toll Like Receptor 3 (TLR3) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll Like Receptor 5 (TLR5) Cascade TAS Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade TAS Reactome
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome
Toll Like Receptor TLR1:TLR2 Cascade TAS Reactome
Toll Like Receptor TLR6:TLR2 Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation TAS Reactome
TRIF (TICAM1)-mediated TLR4 signaling TAS Reactome





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