Experiment description of studies that identified CD40 in exosomes

1 Experiment ID 47 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD81|CD86 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method FACS PubMed ID 17641064     Organism Homo sapiens Experiment description Exosomes with immune modulatory features are present in human breast milk. Authors Admyre C, Johansson SM, Qazi KR, Filen JJ, Lahesmaa R, Norman M, Neve EP, Scheynius A, Gabrielsson S Journal name JIMMU Publication year 2007 Sample B cells Sample name B cells Isolation/purification methods Differential centrifugation Filtration Sucrose density gradient Flotation density - Molecules identified in the study Protein Methods used in the study FACS

2 Experiment ID 79 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD81|MHCII EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20458337     Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting

3 Experiment ID 80 ISEV standards ✘ EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD81|MHCII EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method Mass spectrometry PubMed ID 20458337     Organism Homo sapiens Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2 Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. Journal name ICB Publication year 2010 Sample B cells Sample name RN (HLA-DR15) Isolation/purification methods Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) Flotation density - Molecules identified in the study Protein Methods used in the study Mass spectrometry [FT-ICR] Western blotting

4 Experiment ID 50 ISEV standards ✔ EM|IEM EV Biophysical techniques ✘ EV Cytosolic markers ✔ CD63|CD87 EV Membrane markers ✘ EV Negative markers ✘ EV Particle analysis Identified molecule protein Identification method FACS PubMed ID 14582906     Organism Homo sapiens Experiment description Exosomes with major histocompatibility complex class II and co-stimulatory molecules are present in human BAL fluid. Authors Admyre C, Grunewald J, Thyberg J, Gripenback S, Tornling G, Eklund A, Scheynius A, Gabrielsson S Journal name ERJ Publication year 2003 Sample Dendritic cells Sample name Monocyte-derived dendritic cells Isolation/purification methods Differential centrifugation Immunobeads (EpCAM) Flotation density - Molecules identified in the study Protein Methods used in the study FACS Immunoelectron Microscopy

Protein-protein interactions for CD40

Protein Interactor ExoCarta ID Identification method PubMed Species 1 CALR 811 Affinity Capture-MS Homo sapiens 2 NCOA6   Reconstituted Complex Homo sapiens 3 SLC39A7   Affinity Capture-MS Homo sapiens 4 TRAF3IP2   Invivo Homo sapiens Affinity Capture-MS Homo sapiens 5 TRAF6 7189 Invivo Homo sapiens Invitro Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 6 SGK1   Affinity Capture-MS Homo sapiens 7 POLR2H 5437 Affinity Capture-MS Homo sapiens 8 UGGT1 56886 Affinity Capture-MS Homo sapiens 9 USP15 9958 Affinity Capture-MS Homo sapiens 10 DDX3X 1654 Affinity Capture-MS Homo sapiens 11 MS4A1 931 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 12 TRAF2   Co-crystal Structure Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 13 RFC5 5985 Affinity Capture-MS Homo sapiens 14 TRAF3 7187 Invivo Homo sapiens Invivo Homo sapiens Invivo Homo sapiens Invitro Homo sapiens Invitro Homo sapiens Invitro Homo sapiens Reconstituted Complex Homo sapiens 15 ERP44 23071 Affinity Capture-MS Homo sapiens 16 TXN 7295 Affinity Capture-MS Homo sapiens 17 RIPK2   Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens 18 TRAF5   Invitro Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 19 SLC30A7 148867 Affinity Capture-MS Homo sapiens 20 CD40LG 959 Invivo Homo sapiens Invivo Homo sapiens Invitro Homo sapiens Invitro Homo sapiens 21 XRCC6 2547 Invitro Homo sapiens Affinity Capture-MS Homo sapiens 22 TDP2 51567 Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-Western Homo sapiens 23 FBL 2091 Affinity Capture-MS Homo sapiens 24 C13orf7   Affinity Capture-MS Homo sapiens 25 XRCC5 7520 Invitro Homo sapiens 26 SUMO1 7341 Two-hybrid Homo sapiens 27 TRAF1 7185 Invivo Homo sapiens Invitro Homo sapiens View the network

Pathways in which CD40 is involved

No pathways found