ExoCarta: Gene summary

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Gene description for OTC
Gene name	ornithine carbamoyltransferase
Gene symbol	OTC
Other names/aliases	OCTD
Species	Homo sapiens
Database cross references - OTC
ExoCarta	ExoCarta_5009
Vesiclepedia	VP_5009
Entrez Gene	5009
HGNC	8512
MIM	300461
UniProt	P00480
OTC identified in exosomes derived from the following tissue/cell type
Chondrocytes	35931686
Chondrocytes	35931686
Mesenchymal stem cells	Unpublished / Not applicable

Gene ontology annotations for OTC

Molecular Function
	ornithine carbamoyltransferase activity	GO:0004585	IBA
	ornithine carbamoyltransferase activity	GO:0004585	IDA
	phospholipid binding	GO:0005543	IEA
	amino acid binding	GO:0016597	IEA
	phosphate ion binding	GO:0042301	IEA
	identical protein binding	GO:0042802	IEA
Biological Process
	urea cycle	GO:0000050	IDA
	urea cycle	GO:0000050	IEA
	liver development	GO:0001889	IEA
	L-arginine biosynthetic process	GO:0006526	IEA
	ornithine catabolic process	GO:0006593	IDA
	midgut development	GO:0007494	IEA
	response to xenobiotic stimulus	GO:0009410	IEA
	response to zinc ion	GO:0010043	IEA
	citrulline biosynthetic process	GO:0019240	IBA
	citrulline biosynthetic process	GO:0019240	IDA
	response to insulin	GO:0032868	IEA
	arginine biosynthetic process via ornithine	GO:0042450	IBA
	monoatomic anion homeostasis	GO:0055081	IEA
	response to biotin	GO:0070781	IEA
	ammonium homeostasis	GO:0097272	IMP
Subcellular Localization
	mitochondrion	GO:0005739	HTP
	mitochondrion	GO:0005739	IBA
	mitochondrion	GO:0005739	IDA
	mitochondrial inner membrane	GO:0005743	IEA
	mitochondrial matrix	GO:0005759	NAS
	mitochondrial matrix	GO:0005759	TAS

Experiment description of studies that identified OTC in exosomes

Experiment ID

494

MISEV standards
✔ EM	Biophysical techniques
✔ CD9\|CD81\|CD151\|CD63\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|SDCBP\|TFRC\|TSG101	Enriched markers
✘	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

"Varela-EirÃn M, Carpintero-FernÃ¡ndez P, GuitiÃ¡n-CaamaÃ±o A, Varela-VÃ¡zquez A, GarcÃa-Yuste A, SÃ¡nchez-Temprano A, Bravo-LÃ³pez SB, YaÃ±ez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, MayÃ¡n MD."

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

Osteoarthritic cartilage

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

496

MISEV standards
✘	Biophysical techniques
✔ CD9\|CD151\|CD63\|CD81\|CD82\|FLOT1\|FLOT2\|GAPDH\|LAMP1\|LAMP2\|SDCBP\|TFRC\|TSG101	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

35931686

Organism

Homo sapiens

Experiment description

Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression

Authors

Journal name

Cell Death Dis

Publication year

2022

Sample

Chondrocytes

Sample name

Healthy cartilage

Isolation/purification methods

Differential centrifugation
Filtration
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectometry

Experiment ID

126

MISEV standards
✘	Biophysical techniques
✔ GAPDH	Enriched markers
✘	Negative markers
✘	Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry [LTQ-FT Ultra]

PubMed ID

Unpublished / Not applicable

Organism

Homo sapiens

Experiment description

Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?

Authors

"Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"

Journal name

Mesenchymal Stem Cell Therapy

Publication year

2011

Sample

Mesenchymal stem cells

Sample name

huES9.E1

Isolation/purification methods

HPLC

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Antobody array
Mass spectrometry

Protein-protein interactions for OTC

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

MRPS27

23107

Proximity Label-MS

Homo sapiens

AFG3L2

10939

Proximity Label-MS

Homo sapiens

NDUFS1

4719

Proximity Label-MS

Homo sapiens

MRPL37

51253

Proximity Label-MS

Homo sapiens

ETFB

2109

Proximity Label-MS

Homo sapiens

FDXR

2232

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NDUFS2

4720

Proximity Label-MS

Homo sapiens

LRPPRC

10128

Proximity Label-MS

Homo sapiens

POTEE

445582

Proximity Label-MS

Homo sapiens

NDUFS3

4722

Proximity Label-MS

Homo sapiens

OGDH

4967

Proximity Label-MS

Homo sapiens

IARS2

55699

Proximity Label-MS

Homo sapiens

NDUFA7

4701

Proximity Label-MS

Homo sapiens

HARS2

23438

Affinity Capture-MS

Homo sapiens

MRPS5

64969

Proximity Label-MS

Homo sapiens

MRPS23

51649

Proximity Label-MS

Homo sapiens

NDUFA5

4698

Proximity Label-MS

Homo sapiens

MRPL12

6182

Proximity Label-MS

Homo sapiens

ETFA

2108

Proximity Label-MS

Homo sapiens

MRPS26

64949

Proximity Label-MS

Homo sapiens

NDUFV1

4723

Proximity Label-MS

Homo sapiens

NDUFV3

4731

Proximity Label-MS

Homo sapiens

MRPS7

51081

Proximity Label-MS

Homo sapiens

TACO1

Proximity Label-MS

Homo sapiens

DLAT

1737

Proximity Label-MS

Homo sapiens

NDUFA6

Proximity Label-MS

Homo sapiens

MTIF2

4528

Proximity Label-MS

Homo sapiens

ATPAF1

Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

STOML2

30968

Proximity Label-MS

Homo sapiens

MRPS25

64432

Proximity Label-MS

Homo sapiens

NDUFS4

4724

Proximity Label-MS

Homo sapiens

MRPS34

65993

Proximity Label-MS

Homo sapiens

ACOT2

10965

Proximity Label-MS

Homo sapiens

CPS1

1373

Affinity Capture-MS

Homo sapiens

PTCD3

55037

Proximity Label-MS

Homo sapiens

MMAB

326625

Proximity Label-MS

Homo sapiens

MRPS6

Proximity Label-MS

Homo sapiens

VWA8

23078

Proximity Label-MS

Homo sapiens

POLDIP2

26073

Proximity Label-MS

Homo sapiens

PCLO

27445

Proximity Label-MS

Homo sapiens

KIAA0391

Proximity Label-MS

Homo sapiens

NARS2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FAM127A

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MCCC1

56922

Affinity Capture-MS

Homo sapiens

NDUFAF7

Affinity Capture-MS

Homo sapiens

NME4

4833

Proximity Label-MS

Homo sapiens

ATP5B

506

Proximity Label-MS

Homo sapiens

ACAT1

Proximity Label-MS

Homo sapiens

DAP3

7818

Proximity Label-MS

Homo sapiens

TRMT10C

54931

Proximity Label-MS

Homo sapiens

MRPS35

60488

Proximity Label-MS

Homo sapiens

NDUFA9

4704

Proximity Label-MS

Homo sapiens

LETM1

3954

Proximity Label-MS

Homo sapiens

MRPS14

Proximity Label-MS

Homo sapiens

NFS1

9054

Proximity Label-MS

Homo sapiens

ATP5F1

515

Proximity Label-MS

Homo sapiens

PYCR1

5831

Proximity Label-MS

Homo sapiens

ELAC2

60528

Proximity Label-MS

Homo sapiens

VARS2

Proximity Label-MS

Homo sapiens

PYCR2

29920

Proximity Label-MS

Homo sapiens

ECHS1

1892

Proximity Label-MS

Homo sapiens

MRPS28

Proximity Label-MS

Homo sapiens

DLST

1743

Proximity Label-MS

Homo sapiens

PREPL

9581

Proximity Label-MS

Homo sapiens

MRPS36

92259

Proximity Label-MS

Homo sapiens

PDPR

55066

Proximity Label-MS

Homo sapiens

MRPS17

51373

Proximity Label-MS

Homo sapiens

NDUFV2

4729

Proximity Label-MS

Homo sapiens

TOMM20

9804

Reconstituted Complex

Homo sapiens

PPIF

10105

Proximity Label-MS

Homo sapiens

NIPSNAP3A

Affinity Capture-MS

Homo sapiens

GLS

2744

Affinity Capture-MS		Homo sapiens
Proximity Label-MS		Homo sapiens

GLUD1

2746

Proximity Label-MS

Homo sapiens

CLPX

10845

Proximity Label-MS

Homo sapiens

MRPS2

51116

Proximity Label-MS

Homo sapiens

POLG

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ATP5J

522

Proximity Label-MS

Homo sapiens

NDUFAF2

Proximity Label-MS

Homo sapiens

COQ5

Proximity Label-MS

Homo sapiens

UQCC1

55245

Proximity Label-MS

Homo sapiens

LARS2

23395

Proximity Label-MS

Homo sapiens

MRPS31

Proximity Label-MS

Homo sapiens

PDHA1

5160

Proximity Label-MS

Homo sapiens

NDUFA12

55967

Proximity Label-MS

Homo sapiens

PDHB

5162

Proximity Label-MS

Homo sapiens

MRPS10

55173

Proximity Label-MS

Homo sapiens

NUDT19

390916

Proximity Label-MS

Homo sapiens

SHMT2

6472

Proximity Label-MS

Homo sapiens

TUBB1

81027

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

NDUFS8

4728

Proximity Label-MS

Homo sapiens

MRPS18B

28973

Proximity Label-MS

Homo sapiens

NDUFA2

4695

Proximity Label-MS

Homo sapiens

ACOT1

641371

Proximity Label-MS

Homo sapiens

MRPS16

Proximity Label-MS

Homo sapiens

NDUFAF4

29078

Proximity Label-MS

Homo sapiens

MRPS24

64951

Proximity Label-MS

Homo sapiens

MRPS22

56945

Proximity Label-MS

Homo sapiens

NDUFS6

Proximity Label-MS

Homo sapiens

MRPS9

64965

Proximity Label-MS

Homo sapiens

100

TIMM44

10469

Proximity Label-MS

Homo sapiens

101

NDUFS7

374291

Proximity Label-MS

Homo sapiens

View the network

Pathways in which OTC is involved

Pathway	Evidence	Source
Metabolism	TAS	Reactome
Metabolism of amino acids and derivatives	TAS	Reactome
Mitochondrial protein import	IEA	Reactome
Mitochondrial protein import	TAS	Reactome
Protein localization	IEA	Reactome
Protein localization	TAS	Reactome
Urea cycle	TAS	Reactome

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