Gene description for TMSB4X
Gene name thymosin beta 4, X-linked
Gene symbol TMSB4X
Other names/aliases FX
PTMB4
TB4X
TMSB4
Species Homo sapiens
 Database cross references - TMSB4X
ExoCarta ExoCarta_7114
Entrez Gene 7114
HGNC 11881
MIM 300159
UniProt P62328  
 TMSB4X identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for TMSB4X
Molecular Function
    actin monomer binding GO:0003785 IEA
    protein binding GO:0005515 IPI
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    platelet activation GO:0030168 TAS
    platelet degranulation GO:0002576 TAS
    regulation of cell migration GO:0030334 IEA
    actin filament organization GO:0007015 IEA
    blood coagulation GO:0007596 TAS
    sequestering of actin monomers GO:0042989 IEA
Subcellular Localization
    cytosol GO:0005829 IEA
    extracellular region GO:0005576 TAS
    cytoskeleton GO:0005856 IEA
    nucleus GO:0005634 IEA
    platelet alpha granule lumen GO:0031093 TAS
 Experiment description of studies that identified TMSB4X in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for TMSB4X
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACTA1 58
Reconstituted Complex Homo sapiens
2 PPP4C 5531
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 ACTG1 71
Invivo Homo sapiens
Invitro Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which TMSB4X is involved
PathwayEvidenceSource
Platelet degranulation TAS Reactome





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